Development of novel monitoring system for quorum sensing mediators in Gram-negative bacteria
Project/Area Number |
20590041
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Physical pharmacy
|
Research Institution | Nihon University |
Principal Investigator |
MIYAIRI Shinichi Nihon University, 薬学部, 教授 (50209855)
|
Co-Investigator(Kenkyū-buntansha) |
SAITO Hiroaki 日本大学, 薬学部, 助教 (30385976)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | グラム陰性菌 / 緑膿菌 / クオラムセンシング / オートインデューサー / 高速液体クロマトグラフィー / 蛍光誘導体化 / エラスターゼ活性測定法 / 蛍光標識化試薬 / エラスターゼ / ピオシアニン / マクロライド系抗生物質 / 毒性発現因子 / エラスターゼ合成基質 / ELISA |
Research Abstract |
In this project we have developed a convenient and simultaneous determination system of 3-oxo-acyl-HSLs, those are autoinducer molecules of Gram-negative bacteria, by high-performance liquid chromatography with fluorescence derivatization. Then we have clarified the relation of AI concentration, elastase activity and cell density using Pseudomonas aeruginosa, one of Gram-negative bacteria. The results are that AI concentration was well synchronized with cell growth and there were 2 hours-time lag between increasing of AI concentration and that of elastase activity.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Roles of Pseudomonas aeruginosa autoinducers and their degradation products, tetramic acids, in bacterial survival and behavior in ecological niches.2011
Author(s)
N.Hosono, S.Kimura, K.Tateda, M.Horikawa, C.Ueda, Y.Ishii, M.Ishiguro, S.Miyairi, K.Yamaguchi
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Journal Title
Microbes and Environments
NAID
Related Report
Peer Reviewed
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