The identification and characterization of proteins that are S-nitrosylated during bacterial infection and sepsis

• Project/Area Number: 20590052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: NISHIYA Tadashi Hokkaido University, 大学院・医学研究科, 講師 (80399831)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: iNOS / SPSB / ユビキチン化 / 蛋白質分解 / 敗血症 / ubiquitination / SSB / S-ニトロシル化

Research Abstract

We have identified SPSB family of proteins as an iNOS-binding protein. SPSB family of proteins function as an adaptor protein that bridges between iNOS and the Elongin B/C-Cul5-type E3 ubiquitin ligase, and induce the ubiquitin/proteasome-dependent degradation of iNOS. SPSB family of proteins play an essential role in protection against the cytotoxic effect of iNOS.

Research Products

• [Journal Article] Regulation of inducible nitric oxide synthase by the SPRY domain- and SOCS box-containing proteins (2011)
  • Author(s): Nishiya T, Matsumoto K, Maekawa S, Kajita E, Fujimuro M, Ogasawara K, Uehara T, Miwa S
  • Journal Title: J Biol Chem 286 Pages: 9009-9019
  • Peer Reviewed
• [Journal Article] Regulation of inducible nitric-oxide synthase by the SPRY domain-and SOCS box-containg Proteins (2011)
  • Author(s): Nishiya T, et al.
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Pages: 9009-9019
  • Peer Reviewed
• [Journal Article] Screening systems for the identification of S-nitrosylated proteins (2011)
  • Author(s): Uehara T, Nishiya T
  • Journal Title: Nitric Oxide Volume: (In press,In press)
  • Peer Reviewed
• [Journal Article] The ECS(SPSB) E3 ubiquitin ligase is the master regulator of the lifetuime of inducible nitric-oxide synthase
  • Author(s): Nishiya T, Matsumoto K, Maekawa S, Kajita E, Fujimuro M, Ogasawara K, Uehara T, Miwa S
  • Journal Title: Biochem Biophys Res Commun 印刷中
  • Peer Reviewed
• [Journal Article] Screening systems for the identification of S-nitrosylated proteins
  • Author(s): Uehara T, Nishiya T.
  • Journal Title: Nitric Oxide 印刷中
  • Peer Reviewed
• [Presentation] iNOS regulation by the SPRY domain- and SOCS box-containing proteins (SPSBs) in macrophages (2011)
  • Author(s): 西屋禎
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-23
• [Presentation] Elongin B/C、Cul5、Rbx2、及 びSPRY domain-containing SOCS box protein (SSB)により構成されるE3ユビキチンリガーゼ複合体(ECS-SSB)は誘導型一酸化窒素合成酵素(iNOS)の分解制御システムである (2010)
  • Author(s): 西屋禎
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Elongin B/C、Cu15、Rbx2、及びSPRY domain-containing SOCS box protein (SSB)により構成されるE3ユビキチンリガーゼ複合体(ECS-SSB)は誘導型一酸化窒素合成酵素(iNos)の分解制御システムである (2010)
  • Author(s): 西屋禎, 他
  • Organizer: 第33回日本分子生物学会-第83回日本生化学会合同年会
  • Place of Presentation: ポートピアホテル、神戸
  • Year and Date: 2010-12-09
• [Presentation] The SPRY domain-containing SOCS box protein family regulates the lifetime of iNOS by linking iNOS with Elongin BC-Cul5-Rbx2 E3 ubiquitin ligase complex (2010)
  • Author(s): 西屋禎
  • Organizer: The 14^<th> International Congress of Immunology (ICI2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Presentation] The SPRY domain-containing SOCS box protein family regulates the lifetime of iNOS by Iinking iNOS with elongin BC-Cu15-Rbx2 E3 ubiquitin ligase complex. (2010)
  • Author(s): Nishiya T, et al.
  • Organizer: The 14^<th> International Congress of Immunology(ICI2010)
  • Place of Presentation: Portopia Hotel, Kobe
  • Year and Date: 2010-08-23
• [Presentation] The lifetime of iNOS is regulated by the SPRY domain-containing SOCS box protein family linking iNOS to the Elongin BC-Cul5-Rbx2 E3 ubiquitin ligase complex (2010)
  • Author(s): 西屋禎
  • Organizer: The 6^<th> International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide (NO2010)
  • Place of Presentation: 京都
  • Year and Date: 2010-06-17
• [Presentation] The lifetime of iNOS is regulated by the SPRY domain-containing SOCS box protein family linking iNOS to the elongin BC-Cu15-Rbx2 E3 ubiquitin ligase complex. (2010)
  • Author(s): Nishiya T, et al.
  • Organizer: The 6^<th> International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide (NO2010)
  • Place of Presentation: Kyoto International Conference Center, Kyoto
  • Year and Date: 2010-06-17
• [Book] SIGNALING BY TOLL-LIKE RECEPTORS (2008)
  • Author(s): Tadashi Nishiya
  • Publisher: CRC Press Taylor & Francis group
• [Remarks] ホームページ等
  • URL: http://saibo-yakuri.med.hokudai.ac.jp/seika/seika20110103.html
• [Remarks]
  • URL: http://saibo-yakuri.med.hokudai.ac.jp/kenkyuuseika.html
• [Remarks]
  • URL: http://saibo-yakuri.med.hokudai.ac.jp/research/research_04.html