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The identification and characterization of proteins that are S-nitrosylated during bacterial infection and sepsis

Research Project

Project/Area Number 20590052
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionHokkaido University

Principal Investigator

NISHIYA Tadashi  Hokkaido University, 大学院・医学研究科, 講師 (80399831)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsiNOS / SPSB / ユビキチン化 / 蛋白質分解 / 敗血症 / ubiquitination / SSB / S-ニトロシル化
Research Abstract

We have identified SPSB family of proteins as an iNOS-binding protein. SPSB family of proteins function as an adaptor protein that bridges between iNOS and the Elongin B/C-Cul5-type E3 ubiquitin ligase, and induce the ubiquitin/proteasome-dependent degradation of iNOS. SPSB family of proteins play an essential role in protection against the cytotoxic effect of iNOS.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Regulation of inducible nitric oxide synthase by the SPRY domain- and SOCS box-containing proteins2011

    • Author(s)
      Nishiya T, Matsumoto K, Maekawa S, Kajita E, Fujimuro M, Ogasawara K, Uehara T, Miwa S
    • Journal Title

      J Biol Chem 286

      Pages: 9009-9019

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of inducible nitric-oxide synthase by the SPRY domain-and SOCS box-containg Proteins2011

    • Author(s)
      Nishiya T, et al.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286 Pages: 9009-9019

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening systems for the identification of S-nitrosylated proteins2011

    • Author(s)
      Uehara T, Nishiya T
    • Journal Title

      Nitric Oxide

      Volume: (In press,In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The ECS(SPSB) E3 ubiquitin ligase is the master regulator of the lifetuime of inducible nitric-oxide synthase

    • Author(s)
      Nishiya T, Matsumoto K, Maekawa S, Kajita E, Fujimuro M, Ogasawara K, Uehara T, Miwa S
    • Journal Title

      Biochem Biophys Res Commun 印刷中

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening systems for the identification of S-nitrosylated proteins

    • Author(s)
      Uehara T, Nishiya T.
    • Journal Title

      Nitric Oxide 印刷中

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] iNOS regulation by the SPRY domain- and SOCS box-containing proteins (SPSBs) in macrophages2011

    • Author(s)
      西屋禎
    • Organizer
      第84回日本薬理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Elongin B/C、Cul5、Rbx2、及 びSPRY domain-containing SOCS box protein (SSB)により構成されるE3ユビキチンリガーゼ複合体(ECS-SSB)は誘導型一酸化窒素合成酵素(iNOS)の分解制御システムである2010

    • Author(s)
      西屋禎
    • Organizer
      第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Elongin B/C、Cu15、Rbx2、及びSPRY domain-containing SOCS box protein (SSB)により構成されるE3ユビキチンリガーゼ複合体(ECS-SSB)は誘導型一酸化窒素合成酵素(iNos)の分解制御システムである2010

    • Author(s)
      西屋禎, 他
    • Organizer
      第33回日本分子生物学会-第83回日本生化学会合同年会
    • Place of Presentation
      ポートピアホテル、神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] The SPRY domain-containing SOCS box protein family regulates the lifetime of iNOS by linking iNOS with Elongin BC-Cul5-Rbx2 E3 ubiquitin ligase complex2010

    • Author(s)
      西屋禎
    • Organizer
      The 14^<th> International Congress of Immunology (ICI2010)
    • Place of Presentation
      神戸
    • Year and Date
      2010-08-23
    • Related Report
      2010 Final Research Report
  • [Presentation] The SPRY domain-containing SOCS box protein family regulates the lifetime of iNOS by Iinking iNOS with elongin BC-Cu15-Rbx2 E3 ubiquitin ligase complex.2010

    • Author(s)
      Nishiya T, et al.
    • Organizer
      The 14^<th> International Congress of Immunology(ICI2010)
    • Place of Presentation
      Portopia Hotel, Kobe
    • Year and Date
      2010-08-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] The lifetime of iNOS is regulated by the SPRY domain-containing SOCS box protein family linking iNOS to the Elongin BC-Cul5-Rbx2 E3 ubiquitin ligase complex2010

    • Author(s)
      西屋禎
    • Organizer
      The 6^<th> International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide (NO2010)
    • Place of Presentation
      京都
    • Year and Date
      2010-06-17
    • Related Report
      2010 Final Research Report
  • [Presentation] The lifetime of iNOS is regulated by the SPRY domain-containing SOCS box protein family linking iNOS to the elongin BC-Cu15-Rbx2 E3 ubiquitin ligase complex.2010

    • Author(s)
      Nishiya T, et al.
    • Organizer
      The 6^<th> International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide (NO2010)
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report
  • [Book] SIGNALING BY TOLL-LIKE RECEPTORS2008

    • Author(s)
      Tadashi Nishiya
    • Publisher
      CRC Press Taylor & Francis group
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://saibo-yakuri.med.hokudai.ac.jp/seika/seika20110103.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://saibo-yakuri.med.hokudai.ac.jp/kenkyuuseika.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://saibo-yakuri.med.hokudai.ac.jp/research/research_04.html

    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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