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Identification of novel Gβγ dependent RhoGEF interacting molecules

Research Project

Project/Area Number 20590055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionGifu University

Principal Investigator

UEDA Hiroshi  Gifu University, 工学部, 准教授 (50253779)

Co-Investigator(Kenkyū-buntansha) BANNO Yoshiko  岐阜大学, 医学(系)研究科(研究院), 准教授 (50116852)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsRhoGEF / 三量体G蛋白質 / Rhoファミリー低分子量G蛋白質 / アクチン細胞骨格 / 遺伝子制御 / 細胞形態変化 / GPCR
Research Abstract

Rho family GTPase-specific guanine nucleotide exchange factors of Dbl family regulate avariety of cellular events including cytoskeletal arrangement, signal transduction, and gene expression through activation of Rho family GTPases. FLJ00018/CLG/PLEKHG2 is a guanine nucleotide exchange factor (GEF) for Rho family of small GTPases and Dbl homology (DH), Pleckstrin homology (PH) domains. FLJ00018 possesses a region that binds to heterotrimeric GTP-binding protein βγ subunits. This interaction activates the GEF activity of FLJ00018, and thereby enhances serum response element (SRE) dependent gene transcription and affects cell spreading. In this research project, we have examined the functional significance of the PH domain of FLJ00018. We show here that the PH domain of FLJ00018 interact to phosphoinositide containing PtdIns(4,5)P2, PtdIns(3,4,5)P3 and phosphatidic acid. Phosopholipase C and phospholipase D regulate FLJ00018 functions through the PH domain.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] TRPM1 : A vertebrate TRP channel responsible for retinal ON bipolar function.2010

    • Author(s)
      Koike C., Numata T., Ueda H., Mori Y., Furukawa T.
    • Journal Title

      Cell Caluciuru 48

      Pages: 95-101

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade.2010

    • Author(s)
      Koike C., Obara T., Uriu Y., Numata T., Sanuki R., Miyata K., Koyasu T., Ueno S., Funabiki K., Tani A., Ueda H., Kondo M., Mori Y., Tachibana M., Furukawa T.
    • Journal Title

      Proc.Natl.Acad.Sci.U.S.A. 107(1)

      Pages: 332-337

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] TRPM1 : A vertebrate TRP channel responsible for retinal ON bipolar function.2010

    • Author(s)
      Koike C., et al.
    • Journal Title

      Cell Calcium

      Volume: 48 Pages: 95-101

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TRPMl is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade2010

    • Author(s)
      Koike C., et.al
    • Journal Title

      Proc.Natl.Acad.Sci.USA 107

      Pages: 332-337

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and AKT activation.2009

    • Author(s)
      Nemoto S., Nakamura M., Osawa Y., Kono S., Itoh Y., Okano Y., Murate T., Hara A., Ueda H., Nozawa Y., Banno Y.
    • Journal Title

      J Biol.Chem. 284

      Pages: 10422-10432

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and AKT activation.2009

    • Author(s)
      Nemoto S., et. al
    • Journal Title

      Journal of Biological Chmistry (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Gβγと相互作用するRhoGEF, GBGEF3の活性調節機構2010

    • Author(s)
      佐藤克哉, 他4名
    • Organizer
      BMB2010(第33回日本分子生物学会年会第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Gβγと相互作用するRhoGEF, GBGEF3の活性制御機構(ポスター発表)2010

    • Author(s)
      佐藤克哉、柴田裕貴、長瀬隆弘、上田浩
    • Organizer
      第33回日本分子生物学学会年会第83回日本生化学会大会合同大会BMB2010
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規GPCRシグナル依存性Rho活性化因子の細胞機能における役割(口頭発表)2010

    • Author(s)
      上田浩
    • Organizer
      先端創薬医療シンポジウム
    • Place of Presentation
      岐阜
    • Related Report
      2010 Final Research Report
  • [Presentation] Rho活性化因子FLJ00018EGF受容体transactivationを介した活性化機構2009

    • Author(s)
      佐藤克哉, 他
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Rho活性化因子FLJOOO18のEGF受容体transactivationを介した活性化機構(ポスター発表)2009

    • Author(s)
      佐藤克哉、加藤久司、高野英悟、長瀬隆弘、上田浩
    • Organizer
      第82回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] GiによるRhoファミリー低分子G蛋白質活性化因子の活性制御(ボスター発表)2009

    • Author(s)
      加藤久司、佐藤克哉、高野英悟、長瀬隆弘、上田浩
    • Organizer
      第82回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規βγ依存的RhoA活性化因子のSrcによる活性制御(ポスター発表)2009

    • Author(s)
      高野英悟、佐藤克哉、加藤久司、長瀬隆弘、上田浩
    • Organizer
      第82回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] 三量体G蛋白質βγ依存性RhoGEF, FLJ00018のPDZドメイン結合モチーフを介する活性制御の検討2008

    • Author(s)
      上田浩, その, 他
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] 三量体G蛋白質βγ依存性RhoGEF,FLJ00018のPDZドメイン結合モチーフを介する活性制御の検討(ポスター発表)2008

    • Author(s)
      上田浩、中峰康揮、夏目陽子、佐藤克哉、長瀬隆弘、小原収、吉田敏
    • Organizer
      第31回日本分子生物学学会年会第81回日本生化学会大会合同大会BMB2008
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://wwwl.gifu-u.ac.jp/~mb3_ulab/

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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