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Analysis of melanoma metastasis gene, proteolipid protein

Research Project

Project/Area Number 20590069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKeio University

Principal Investigator

SONODA Yoshiko  Keio University, 薬学部, 教授 (30050743)

Co-Investigator(Kenkyū-buntansha) KASAHARA Tadashi  慶應義塾大学, 薬学部, 教授 (60049096)
TAGO Megumi  慶應義塾大学, 薬学部, 講師 (30445192)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsメラノーマ / 転移 / 接着斑キナーゼ / プロテオリピドプロテイン / PLP2 / FAK / melanoma / B16F10 / B16BL6 / metastasis / B16BL-6
Research Abstract

We established melanoma cells overexpressing proteolipid protein 2 (PLP2). We found that PLP2 enhanced adhesion, motility, and invasion in vitro, and tumor metastasis in vivo. PLP2 siRNA treatment remarkably inhibited adhesion, motility, and invasion in vitro, and prevented tumor metastasis in vivo. Furthermore, we showed that PLP2 binds PI3K, thus activating Akt.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (21 results)

All 2010 2009 2008

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (8 results)

  • [Journal Article] The fixed structure of Licochalcone A by alpha, beta-unsaturated ketone is necessary for anti-inflammatory activity through the inhibition of NF-kappaB activation.2010

    • Author(s)
      Funakoshi-Tago M, Nakamura K, Tsuruya R, Hatanaka M, Mashino T, Sonoda Y, Kasahara T
    • Journal Title

      Int Immunopharmacol. Vol.10

      Pages: 562-71

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteolipid protein 2 is associated with melanoma metastasis.2010

    • Author(s)
      Sonoda Y, Warita M, Suzuki T, Ozawa H, Fukuda Y, Funakoshi-Tago M, Kasahara T
    • Journal Title

      Oncol Rep. Vol.23

      Pages: 371-6

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Licochalcones suppress degranulation by decreasing the intracellular Ca2+ llevel and tyrosine phosphorylation of ERK in RBL-2H3 cells2010

    • Author(s)
      Tanifuji S, Aizu-Yokota E, Funakoshi-Tago M, Sonoda Y, Kasahara T
    • Journal Title

      Int.Immunopharmacol

      Volume: 10 Pages: 769-776

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The fixed structure of licochalcone A by alpha, beta-unsaturated ketone is necessary for anti-inflammatory activity through the inhibition of NF-kappaB activation2010

    • Author(s)
      Funakoshi-Tago M, Nakamura K, Tsuruya R, Hatanaka M, Mashino T, Sonoda Y, Kasahara T
    • Journal Title

      Int Immunopharmacol

      Volume: 10 Pages: 562-571

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteolipid protein 2 is associated with melanoma metastasis2010

    • Author(s)
      Sonoda Y, Warita M.Suzuki T, Ozawa H, Fukuda Y, Funakoshi-Tago M, Kasahara T
    • Journal Title

      Oncol.rep. 23

      Pages: 371-376

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Licochalcone A potently inhibits tumor necrosis factor alpha-induced nuclear factor-kappaB activation through the direct inhibition of IkappaB kinase complex activation.2009

    • Author(s)
      3 Funakoshi-Tago M, Tanabe S, Tago K, Itoh H, Mashino T, Sonoda Y, Kasahara T
    • Journal Title

      Mol Pharmacol. Vol.76

      Pages: 745-53

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] STAT5 activation is critical for the transformation mediated by myeloproliferative disorder-associated JAK2 V617F mutant2009

    • Author(s)
      Funakoshi-tago M, Tago K, Abe M, Sonoda Y, Kasahara T
    • Journal Title

      J.Biol.Chem. 285

      Pages: 5296-5307

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Licochalcone A potently inhibits tumor necrosis factor alpha-induced nuclear kappaB activation through the direct inhibition of IkappaB kinase complex activation2009

    • Author(s)
      Funakoshi-tago M, Tanabe S, Tago K, Itoh H, Mashino T, Sonoda Y, Kasahara T
    • Journal Title

      Mol.Pharmacol. 76

      Pages: 745-753

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glycyrrhiza inflata-derived chalcones, Licochalcone A, Licochalcone B and Licochalcone D, inhibit phosphorylation ofNF-kappaBp65 in IPS signaling pathway.2009

    • Author(s)
      Furusawa J, Funakoshi-TaM, Mashino T, Tago K, Inoue H, Sonoda Y, Kasahara T.
    • Journal Title

      Int Immunopharmacol. 9

      Pages: 499-507

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutation of Y925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis.2008

    • Author(s)
      Kaneda T, Sonoda Y, Ando K, Suzuki T, Sasaki Y, Oshio T, Tago M, Kasahara T
    • Journal Title

      Cancer Lett. Vol.270

      Pages: 354-61

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A synthetic glycosphingolipid-induced antiproliferative effect in melanoma cells is associated with suppression of FAK, Akt, and Erk activation.2008

    • Author(s)
      Sonoda Y, Hada N, Kaneda T, Suzuki T, Ohshio T, Takeda T, Kasahara T.
    • Journal Title

      Biol Pharm Bull. Vol.31

      Pages: 1279-83

    • NAID

      110006684355

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A synthetic glycosphingolipid-induced antiproliferative effect in melanoma cells is associated with suppression ofFAK, Akt, and Erk activation.2008

    • Author(s)
      Sonoda Y, Hada N, Kaneda T, Suzuki T, Ohshio T, Takeda T, Kasahara T.
    • Journal Title

      Biol Pharm Bull. 31

      Pages: 1279-1283

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutation ofY925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis.2008

    • Author(s)
      Kaneda T, Sonoda Y, AndoK, Suzuki T, Sasaki Y, Oshio T, Tago M, Kasahara T.
    • Journal Title

      Cancer Lett. 270

      Pages: 354-361

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] FAK及びPLP2ノックダウンによるメラノーマ転移抑制について2010

    • Author(s)
      小澤広樹
    • Organizer
      第33回分子生物学会、第83回生化学会大会合同大会
    • Year and Date
      2010-12-07
    • Related Report
      2010 Final Research Report
  • [Presentation] FAK及びPLP2ノックダウンによるメラノーマ転移抑制について2010

    • Author(s)
      小澤広規、園田よし子、保科直美、田中和之、多胡めぐみ、笠原忠
    • Organizer
      第33回分子生物学会年会、第83回生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] FAK及びPLP2ノックダウンによるメラノーマ転移抑制について2010

    • Author(s)
      小澤広樹
    • Organizer
      日本薬学会第130年会
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] FAK及びPLP2ノックダウンによるメラノーマ転移抑制について2010

    • Author(s)
      小澤広規, 園田よし子, 保科直美, 田中和之, 多胡めぐみ, 笠原忠
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] PLP2のメラノーマ転移における機能解析2009

    • Author(s)
      割田雅子
    • Organizer
      日本薬学会第129年会
    • Year and Date
      2009-03-25
    • Related Report
      2010 Final Research Report
  • [Presentation] PLP2のメラノーマ転移における機能解析2009

    • Author(s)
      割田雅子, 園田よし子
    • Organizer
      日本薬学会129年会
    • Place of Presentation
      京都国際センター
    • Year and Date
      2009-03-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] 接着斑キナーゼ(FAK)のメラノーマ転移における役割解析2008

    • Author(s)
      笠原忠
    • Organizer
      第52回日本薬学会関東支部会
    • Place of Presentation
      東京理科大学
    • Year and Date
      2008-10-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 接着斑キナーゼ(FAK)のメラノーマ転移における役割解析2008

    • Author(s)
      笠原忠, 園田よし子
    • Organizer
      52回日本薬学会関東支部大会
    • Place of Presentation
      東京理科大学
    • Year and Date
      2008-10-04
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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