Research for host-recognition and responses against endotoxin modified by pathogenic bacteria.

• Project/Area Number: 20590073
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Doshisha Women's College of Liberal Arts
• Principal Investigator: KAWASAKI Kiyoshi Doshisha Women's College of Liberal Arts, 薬学部, 教授 (60270641)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エンドトキシン / Toll-like receptor / 細菌 / 外膜 / 潜伏化 / 脱アシル化 / リポ多糖 / 活性調節 / 膜透過性 / 脂質 / シグナル伝達 / 自然免疫

Research Abstract

Regulatory machineries of lipid A modification enzymes were analyzed in this study. Lipid A modification enzyme PagL is usually latent, and the property of PagL to associate with lipopolysaccharide was shown to be important for the latency. Furthermore, the biological significance of the PagL latency was examined. PagL latency was shown to be beneficial for bacteria to produce effective membrane permeation barrier.

Research Products

• [Journal Article] Latency of the lipid A deacylase PagL is involved in producing a robust permeation barrier in the outer membrane of Salmonella enterica. (2010)
  • Author(s): Kiyoshi Kawasaki, Takayuki Manabe
  • Journal Title: J.Bacteriol. vol.192 Pages: 5837-5840
  • Peer Reviewed
• [Journal Article] Mutations in the lipid A deacylase PagL which release the enzyme from its latency affect the ability of PagL to interact with lipopolysaccharide in Salmonella enterica serovar Typhimurium. (2010)
  • Author(s): Takayuki Manabe, Miyuki Kawano, Kiyoshi Kawasaki
  • Journal Title: Biochem.Biophys.Res.Commun. vol.396 Pages: 812-816
  • Peer Reviewed
• [Journal Article] Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages (2010)
  • Author(s): Miyuki Kawano, Takayuki Manabe, Kiyoshi Kawasaki
  • Journal Title: FEBS lett. vol.584 Pages: 207-212
  • Peer Reviewed
• [Journal Article] Mutations in the lipid A deacylase PagL which release the enzyme from its latency affect the ability of PagL to interact with lipopolysaccharide in Salmonella enterica serovar Typhimurium (2010)
  • Author(s): Manabe T., 他2名
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 396 Pages: 812-816
  • Peer Reviewed
• [Journal Article] Latency of the lipid A deacylase PagL is involved in producing a robust permeation barrier in the outer membrane of Salmonella enterica (2010)
  • Author(s): Kawasaki K., 他1名
  • Journal Title: J.Bacteriol. Volume: 192 Pages: 5837-5840
  • Peer Reviewed
• [Journal Article] Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages. (2010)
  • Author(s): M.Kawano, 他2名
  • Journal Title: FEBS lett. 584 Pages: 207-212
  • Peer Reviewed
• [Journal Article] Mutations in the lipid A deacylase PagL which release the enzyme from its latency affect the ability of PagL to interact with lipopolysaccharide in Salmonella enterica serovar Typhimurium (2010)
  • Author(s): Manabe T., 他2名
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 396 Pages: 812-816
  • Peer Reviewed
• [Journal Article] Latency of the lipid A deacylase PagL is involved in producing a robust permeation barrier in the outer membrane of Salmonella enterica (2010)
  • Author(s): Kawasaki K., 他1名
  • Journal Title: J. Bacteriol. Volume: 192 Pages: 5837-5840
  • Peer Reviewed
• [Journal Article] Alternative procedures for analysis of lipid A modification with phosphoethanolamine or aminoarabinose (2009)
  • Author(s): Kiyoshi Kawasaki
  • Journal Title: J.Microbiol.Methods vol.76 Pages: 313-315
  • Peer Reviewed
• [Journal Article] Alternative procedures for analysis of lipid A modification with phosphoethanolamine or aminoarabinose (2009)
  • Author(s): Kiyoshi Kawasaki
  • Journal Title: J. Microbiol. Methods 6 Pages: 313-315
  • Peer Reviewed
• [Journal Article] The preparation of a lipidic endotoxin affects its biological activities (2008)
  • Author(s): Kazuo Okemoto, Kentaro Hanada, Masahiro Nishijima, Kiyoshi Kawasaki
  • Journal Title: Biol.Pharm.Bull. vol.31 Pages: 1952-1954
  • NAID: 110006936340
  • Peer Reviewed
• [Journal Article] Extracellular loops of lipid A 3-O-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonella enterica serovar Typhimurium. (2008)
  • Author(s): T. Manabe, K. Kawasaki
  • Journal Title: J.Bacteriol. vol.190 Pages: 5597-5606
  • Peer Reviewed
• [Journal Article] Extracellular loops of lipid A 3-Ο-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonell a enterica serovar Typhimurium. (2008)
  • Author(s): T. Manabe, 他1名
  • Journal Title: J. Bacteriol. 190 Pages: 5597-5606
  • Peer Reviewed
• [Journal Article] The preparation of a lipidic endoxon in affects its biological activities (2008)
  • Author(s): Kazuo Okemoto, 他3名
  • Journal Title: Biol. Pharm. Bull. 31 Pages: 1952-1954
  • Peer Reviewed
• [Presentation] Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages (2010)
  • Author(s): Kawasaki K., 他1名
  • Organizer: Annual meetings of the Society for Leukocyte biology and The international Endotoxin and Innate Immunity Society
  • Place of Presentation: バンクーバー、カナダ
  • Year and Date: 2010-09-30
• [Presentation] サルモネラリポ多糖脱アシル化酵素PagLの活性抑制にはリポ多糖との結合が重要である (2010)
  • Author(s): 眞鍋貴行、河野美幸、川崎清史
  • Organizer: 第83回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] 二成分制御系PhoP-PhoQ活性化によるサルモネラ外膜透過性障壁の強化に外膜脱アシル化酵素の潜伏性が関与する (2010)
  • Author(s): 川崎清史、眞鍋貴行
  • Organizer: 第83回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages. (2010)
  • Author(s): K. Kawasaki, M. Kawano
  • Organizer: Annual meetings of the Society for Leukocyte biology and The international Endotoxin and Innate Immunity Society
  • Place of Presentation: バンクーバー(カナダ)
• [Presentation] サルモネラ外膜リパーゼ(PagL)の潜伏性に関わるアミノ酸残基の解析 (2010)
  • Author(s): 川崎清史、眞鍋貴行
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 群馬県渋川市
• [Presentation] Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages (2010)
  • Author(s): Kawasaki K., 他1名
  • Organizer: Annual meetings of the Society for Leukocyte biology and The international Endotoxin and Innate I mmunity Society
  • Place of Presentation: バンクーバー、カナダ
• [Presentation] LpxRによるリピドA脱アシル化はサルモネラの効率的な細胞増殖に必要である (2009)
  • Author(s): 河野美幸、眞鍋貴行、川崎清史
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] サルモネラLPS脱アシル化酵素PagLの活性調節に関与するアミノ酸残基は他の外膜タンパク質との相互作用に重要である (2009)
  • Author(s): 眞鍋貴行、河野美幸、川崎清史
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] サルモネラLPS脱アシル化酵素PagLの活性調節に関与するアミノ酸残基は他の外膜タンパク質との相互作用に重要である (2009)
  • Author(s): 眞鍋貴行, 他2名
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸
• [Presentation] LpxRによるリピドA脱アシル化はサルモネラの効率的な細胞増殖に必要である (2009)
  • Author(s): 河野美幸, 他2名
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸
• [Presentation] Extracellular loops of lipid A 3-O-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonella enterica. (2008)
  • Author(s): K. Kawasaki, T. Manabe
  • Organizer: 10th Biennial Meeting of the International Endotoxin and Innate Immunity Society
  • Place of Presentation: エジンバラ(イギリス)
• [Presentation] Exacraoellular loops of lipid A 3-O-deacylase PagL are involved in recognit ionof aminoarabinose-based membrane modifications in Salmonella enterica (2008)
  • Author(s): Kiyoshi Kawasaki, 他1名
  • Organizer: 10^<th>Biennial Meeting of the International Endotoxin and Innate Immunity Society
  • Place of Presentation: Edinburgh, UK