Analysis of responsive factors for oxidative stress mediated by O-GlcNAc modification

• Project/Area Number: 20590082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Tokyo Metropolitan Institute of Gerontology
• Principal Investigator: MIURA YURI Tokyo Metropolitan Institute of Gerontology, 東京都健康長寿医療センター研究所, 主任研究員 (00216574)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: シグナル伝達 / 酸化ストレス / アポトーシス / ストレス応答タンパク質 / 亜ヒ酸ナトリウム / O-GlcNAc / 熱ショックタンパク質 / 放射線 / ストレス / 脳・神経

Research Abstract

In order to clarify the regulation mechanisms by O-GlcNAcylation in the signaling pathways caused by reactive oxygen species, the alteration of O-GlcNAcylated proteins due to oxidative stress and the effects of O-GlcNAcylation on the induction of stress responsive proteins were examined. The results suggest that O-GlcNAcylated proteins play a critical role of oxidative stress-responses, and that the induction of stress responsive protein is possible to be regulated by O-GlcNAcylation.

Research Products

• [Journal Article] Proteomic Analysis of Plasma Proteins in Japanese Semisuper Centenarians. (2011)
  • Author(s): Miura, Y., Sato, Y., Arai, Y., Abe, Y., Takayama, M., Toda, T., Hirose, N., Endo, T.
  • Journal Title: Exp. Gerontol. 46 Pages: 81-85
• [Journal Article] Proteomic Analysis of Plasma Proteins in Japanese Semisuper Centenarians (2011)
  • Author(s): Miura, Y., et al.
  • Journal Title: Exp.Gerontol. Volume: 46 Pages: 81-85
  • Peer Reviewed
• [Journal Article] Translocation of lysosomal cathepsin D caused by oxidative stress or proteasome inhibition in primary cultured neurons and astrocytes. (2010)
  • Author(s): Miura, Y., Sakurai, Y., Hayakawa, M., Shimada, Y., Zempel, H., Sato, Y., Hisanaga, S., Endo, T.
  • Journal Title: Biol. Pharm. Bull 33 Pages: 22-28
  • NAID: 40016900208
• [Journal Article] Survival responses to oxidative stress and aging. (2010)
  • Author(s): Miura, Y., Endo, T.
  • Journal Title: Geriatr. Gerontol. Int. 10 Pages: 1-10
  • NAID: 10027272568
• [Journal Article] Survival responses to oxidative stress and aging. (2010)
  • Author(s): Miura, Y., Endo, T.
  • Journal Title: Geriatr.Gerontol.Int. Volume: 10
  • NAID: 10027272568
  • Peer Reviewed
• [Journal Article] Translocation of lysosomal cathepsin D caused by oxidative stress or proteasome inhibition in primary cultured neurons and astrocytes (2010)
  • Author(s): Miura, Y., et al.
  • Journal Title: Biol.Pharm.Bull 33 Pages: 22-28
  • NAID: 40016900208
  • Peer Reviewed
• [Journal Article] Proteomic approach for biomarker discovery in radioadaptive responses -Age-dependent variations of cell response to low-dose radiation- (2010)
  • Author(s): Miura, Y
  • Journal Title: Biological Sciences in Space 23 Pages: 16-21
  • NAID: 10025115249
  • Peer Reviewed
• [Journal Article] Proteomic approach for biomarker discovery in radioadaptive responses-Age-dependent variations of cell response to low-dose radiation- (2009)
  • Author(s): Miura, Y.
  • Journal Title: Biological Sciences in Space 23 Pages: 17-22
  • NAID: 10025115249
• [Journal Article] Proteomic approach for biomarker discovery in radioadaptive responses-Age-dependent variations of cell response to low-dose radiation- (2009)
  • Author(s): Miura, Y.
  • Journal Title: Biological Sciences in Space (印刷中)
  • NAID: 10025115249
  • Peer Reviewed
• [Presentation] Effects of O-linked N-acetyl glucosamine (O-GlcNAc) modification on oxidative stress-response (2011)
  • Author(s): Takatoshi Satoh, Yuri Miura, Yoko Sakurai, Tamao Endo, Wakako Hiraoka
  • Organizer: 第49回生物物理学会
  • Place of Presentation: 姫路
• [Presentation] Effects of O-linked N-acetylglucosamine (O-GlcNAc) modification on oxidative stress-induced apoptosis (2010)
  • Author(s): Satoh, T., Miura, Y., Sakurai, Y., Endo, T., Hiraoka, W.
  • Organizer: 第48回生物物理学会
  • Place of Presentation: 仙台
• [Presentation] 酸化ストレス応答に及ぼすO-結合型N-アセチルグルコサミン修飾の影響 (2010)
  • Author(s): 佐藤貴俊、三浦ゆり、櫻井洋子、平岡和佳子、遠藤玉夫
  • Organizer: 第63回日本酸化ストレス学会
  • Place of Presentation: 横浜
• [Presentation] 酸化ストレス応答に及ぼすひ結合型N-アセチルグルコサミン修飾の影響 (2010)
  • Author(s): 三浦ゆり, 他4名
  • Organizer: 第63回日本酸化ストレス学会
  • Place of Presentation: 横浜
• [Presentation] O-結合型N-アセチルグルコサミン修飾(O-GlcNAc化)によるアポトーシスの制御 (2009)
  • Author(s): 三浦ゆり、眞後俊幸、櫻井洋子、久永眞市、遠藤玉夫
  • Organizer: 第62回日本酸化ストレス学会
  • Place of Presentation: 福岡
• [Presentation] O-結合型N-アセチルグルコサミン修飾(O-GlcNAc化)によるDNA損傷応答シグナルの制御 (2009)
  • Author(s): 三浦ゆり、櫻井洋子、遠藤玉夫
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
• [Presentation] O-結合型解N-アセチルグルコサミン修飾(O-GlcNAc化)によるアポトーシスの制御 (2009)
  • Author(s): 三浦ゆり
  • Organizer: 第62回日本酸化ストレス学会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
• [Presentation] O-結合型解N-アセチルグルコサミン修飾(O-GlcNAc化)によるDNA損傷応答シグナルの制御 (2009)
  • Author(s): 三浦ゆり
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都国際会議場(京都)
• [Presentation] ATMタンパク質のO-GlcNAc化修飾 (2008)
  • Author(s): 三浦ゆり、櫻井洋子、遠藤玉夫
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州
• [Presentation] ATMタンパク質のO-GlcNAc化修飾 (2008)
  • Author(s): 三浦ゆり
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州国際会議場(北九州)