Mechanisms involved in the development of bronchial smooth muscle hyperresponsiveness in allergic bronchial asthma

• Project/Area Number: 20590093
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Hoshi University
• Principal Investigator: CHIBA Yoshihiko Hoshi University, 薬学部, 准教授 (00287848)
• Co-Investigator(Renkei-kenkyūsha): SAKAI Hiroyasu 星薬科大学, 薬学部, 助教 (00328923)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 気管支喘息 / 気管支平滑筋 / カルシウム感受性 / RhoA / Interleukin-13(IL-13) / Interleukin-13 / STAT6 / SOCS / RNA干渉

Research Abstract

Interleukin-13 (IL-13) is one of the central mediators for development of airway hyperresponsiveness in asthma. However, its effect on bronchial smooth muscle (BSM) is not well known. Recent studies revealed an involvement of RhoA/Rho-kinase in BSM contraction and this pathway has now been proposed as a new target for asthma therapy. The current study demonstrated that IL-13 caused both an augmented BSM contractility and an upregulation of RhoA in BSMs. Thus, IL-13 signaling is critical for development of BSM hyperresponsiveness and that agents that specifically inhibit this pathway in BSM may provide a novel strategy for treatment of asthma.

Research Products

• [Journal Article] Sphingosine-1-phosphate augments agonist-mediated contraction in bronchial smooth muscles of mice. (2011)
  • Author(s): Chiba, Y., Takeuchi, H., Sakai, H., Misawa M.
  • Journal Title: Pharmacol.Rep. 63 Pages: 544-547
• [Journal Article] Involvement of K+ channels in the augmented nasal venous responsiveness to nitric oxide in rat model of allergic rhinitis. (2011)
  • Author(s): Sakai, H., Enzaka, J., Sakai-Oshita, M., Chiba, Y., Misawa, M
  • Journal Title: Microvas.Res. 81 Pages: 129-134
• [Journal Article] Antigen exposure causes activations of signal transducer and activator of transcription 6 (STAT6) and STAT1, but not STAT3, in lungs of sensitized mice. (2011)
  • Author(s): Chiba, Y., Todoroki, M., Misawa, M.
  • Journal Title: Immunopharmacol.Immunotoxicol. 33 Pages: 43-48
• [Journal Article] Antigen exposure causes activations of signal transducer and activator of transcription 6 (STAT6) and STAT1, but not STAT3, in lungs of sensitized mice. (2011)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Immunopharmacol Immunotoxicol. Volume: 33 Pages: 43-48
  • Peer Reviewed
• [Journal Article] Effects of repeated antigen exposure on the expression of enzymes associated with sphingosine-1-phosphate turnover. (2010)
  • Author(s): Chiba, Y., Suzuki, K., Sakai, H., Misawa, M.
  • Journal Title: Open Inflamm.J. 3 Pages: 1-4
• [Journal Article] A functional interaction between CPI-17 and RACK-1 proteins in bronchial smooth muscle cells. (2010)
  • Author(s): Chiba, Y., Tanabe, M., Sakai, H., Kimura, S., Misawa, M.
  • Journal Title: Biochem.Biophys.Res.Commun. 401 Pages: 487-490
• [Journal Article] Augmented PDBu-mediated contraction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. (2010)
  • Author(s): Sakai, H., Kurihara, Y., Hashimoto, Y., Chiba, Y., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 46 Pages: 259-266
  • NAID: 10026983193
• [Journal Article] Involvement of Src family kinase activation in angiotensin II-induced hyperresponsiveness of rat bronchial smooth muscle. (2010)
  • Author(s): Sakai, H., Nishimura, A., Watanabe, Y., Nishizawa, Y., Hashimoto, Y., Chiba, Y., Misawa, M.
  • Journal Title: Peptides 31 Pages: 2216-2221
• [Journal Article] SKI-II, an inhibitor of sphingosine kinase, ameliorates antigen-induced bronchial smooth muscle hyperresponsiveness, but not airway inflammation, in mice. (2010)
  • Author(s): Chiba, Y., Takeuchi, H., Sakai, H., Misawa, M.
  • Journal Title: J.Pharmacol.Sci. 114 Pages: 304-310
  • NAID: 10029890451
• [Journal Article] Sphingosine-1-phosphate aggravates antigen-induced airway inflammation in mice. (2010)
  • Author(s): Chiba, Y., Suzuki, K., Kurihara, E., Uechi, M., Sakai, H., Misawa, M.
  • Journal Title: Open Respir.Med.J. 4 Pages: 82-85
• [Journal Article] Involvement of multiple PKC isoforms in phorbol 12,13-dibutyrate-induced contraction during high K+ depolarization in bronchial smooth muscle of mice. (2010)
  • Author(s): Sakai, H., Kurihara, Y., Hashimoto, Y., Chiba, Y., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 46 Pages: 225-233
  • NAID: 10026983116
• [Journal Article] Augmented venous responsiveness to leukotriene D4 in nasal septal mucosae of repeatedly antigen challenged rats. (2010)
  • Author(s): Sakai, H., Enzaka, J., Sakai-Oshita, M., Chiba, Y., Misawa, M.
  • Journal Title: Eur.J.Pharmacol. 644 Pages: 215-219
• [Journal Article] Phorbol 12,13-dibutyrate-mediated contraction of the stimulated bronchial smooth muscles of mice. (2010)
  • Author(s): Sakai, H., Kurihara, Y., Hashimoto, Y., Chiba, Y., Misawa, M.
  • Journal Title: Respir.Physiol.Neurobiol. 173 Pages: 120-124
• [Journal Article] Induction of RhoA gene expression by interleukin-4 in cultured human bronchial smooth muscle cells. (2010)
  • Author(s): Chiba, Y., Goto, K., Momata, M., Kobayashi, T., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 46 Pages: 217-224
  • NAID: 130000860975
• [Journal Article] Down-regulation of sphingosine-1-phosphate receptors in bronchial smooth muscle of mouse experimental asthma. (2010)
  • Author(s): Chiba, Y., Suzuki, K., Uechi, M., Kurihara, E., Goto, K., Sakai, H., Misawa, M.
  • Journal Title: Pharmacol.Res. 62 Pages: 357-363
• [Journal Article] Angiotensin II induces hyperresponsiveness of bronchial smooth muscle via an activation of p42/44 ERK in rats. (2010)
  • Author(s): Sakai, H., Nishizawa, Y., Nishimura, A., Chiba, Y., Goto, K., Hanazaki, M., Misawa, M.
  • Journal Title: Pflugers Arch.Eur.J.Physiol. 460 Pages: 645-655
• [Journal Article] Mechanism of inhibitory effect of prednisolone on RhoA upregulation in human bronchial smooth muscle cells. (2010)
  • Author(s): Goto, K., Chiba, Y., Sakai, H., Misawa, M.
  • Journal Title: Biol.Pharm.Bull. 33 Pages: 710-713
  • NAID: 130000248024
• [Journal Article] Identification and characterization of rat RhoA gene promoter. (2010)
  • Author(s): Chiba, Y., Goto, K., Matsusue, K., Kimura, S., Misawa, M.
  • Journal Title: J.Pharmacol.Sci. 112 Pages: 467-472
  • NAID: 10027909220
• [Journal Article] Upregulation of geranylgeranyltransferase I in bronchial smooth muscle of mouse experimental asthma : its inhibition by lovastatin. (2010)
  • Author(s): Chiba, Y., Sato, S., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 46 Pages: 57-64
  • NAID: 10026187207
• [Journal Article] Upregulation of interleukin-13 receptor chains in bronchial smooth muscle tissues of mouse experimental asthma. (2010)
  • Author(s): Chiba, Y., Onoda, S., Todoroki, M., Nishida, Y., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 46 Pages: 49-55
  • NAID: 10026187173
• [Journal Article] Effect of a matrix metalloproteinase-12 inhibitor, S-1, on allergic airway disease phenotypes in mice. (2010)
  • Author(s): Yu, Y., Chiba, Y., Sakai, H., Misawa, M.
  • Journal Title: Inflamm.Res. 59 Pages: 419-428
• [Journal Article] The proximal STAT6 and NF-kB sites are responsible for RhoA transcription in human bronchial smooth muscle. (2010)
  • Author(s): Goto, K., Chiba, Y., Matsusue, K., Hattori, Y., Maitani, Y., Sakai, H., Kimura, S., Misawa, M.
  • Journal Title: Pharmacol.Res. 61 Pages: 466-472
• [Journal Article] Possible involvements of NF-kB and AP-1 in the TNF-a-induced upregulation of MMP-12 in human alveolar epithelial A549 cell line. (2010)
  • Author(s): Yu, Y., Chiba, Y., Sakai, H., Misawa, M.
  • Journal Title: J.Pharmacol.Sci. 112 Pages: 83-88
• [Journal Article] Interleukin-4 upregulates RhoA protein via an activation of STAT6 in cultured human bronchial smooth muscle cells. (2010)
  • Author(s): Chiba, Y., Todoroki, M., Misawa, M.
  • Journal Title: Pharmacol.Res. 61 Pages: 188-192
• [Journal Article] Phosphorylation of signal transducer and activator of transcription 6 (STAT6) and STAT1, but not STAT3, induced by antigen inhalation in bronchial smooth muscles of sensitized mice. (2010)
  • Author(s): Chiba, Y., Todoroki, M., Misawa, M.
  • Journal Title: Biol.Pharm.Bull. 33 Pages: 146-149
  • NAID: 130000140210
• [Journal Article] Effect of a matrix metalloproteinase-12 inhibitor, S-1, on allergic airway disease phenotypes in mice. (2010)
  • Author(s): Yu Yingyan
  • Journal Title: Inflamm.Res. Volume: 59 Pages: 419-428
  • Peer Reviewed
• [Journal Article] Upregulation of interleukin-13 receptor chains in bronchial smooth muscle tissues of mouse experimental asthma. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J.Smooth Muscle Res. Volume: 46 Pages: 49-55
  • NAID: 10026187173
  • Peer Reviewed
• [Journal Article] Upregulation of geranylgeranyltransferase I in bronchial smooth muscle of mouse experimental asthma : its inhibition by lovastatin. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J.Smooth Muscle Res. Volume: 46 Pages: 57-64
  • NAID: 10026187207
  • Peer Reviewed
• [Journal Article] Identification and characterization of rat RhoA gene promoter. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J.Pharmacol.Sci. Volume: 112 Pages: 467-472
  • NAID: 10027909220
  • Peer Reviewed
• [Journal Article] Mechanism of inhibitory effect of prednisolone on RhoA upregulation in human bronchial smooth muscle cells. (2010)
  • Author(s): Kumiko Goto
  • Journal Title: Biol.Pharm.Bull. Volume: 33 Pages: 710-713
  • Peer Reviewed
• [Journal Article] Angiotensin II induces hyperresponsiveness of bronchial smooth muscle via an activation of p42/44 ERK in rats. (2010)
  • Author(s): Hiroyasu Sakai
  • Journal Title: Pflugers Arch.Eur.J.Physiol. Volume: 460 Pages: 645-655
  • Peer Reviewed
• [Journal Article] Down-regulation of sphingosine-1-phosphate receptors in bronchial smooth muscle of mouse experimental asthma. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Pharmacol.Res. Volume: 62 Pages: 357-363
  • Peer Reviewed
• [Journal Article] Induction of RhoA gene expression by interleukin-4 in cultured human bronchial smooth muscle cells. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J.Smooth Muscle Res. Volume: 46 Pages: 217-224
  • NAID: 130000860975
  • Peer Reviewed
• [Journal Article] Phorbol 12, 13-dibutyrate-mediated contraction of the stimulated bronchial smooth muscles of mice. (2010)
  • Author(s): Hiroyasu Sakai
  • Journal Title: Respir.Physiol.Neurobiol Volume: 173 Pages: 120-124
  • Peer Reviewed
• [Journal Article] Augmented venous responsiveness to leukotriene D4 in nasal septal mucosae of repeatedly antigen challenged rats. (2010)
  • Author(s): Hiroyasu Sakai
  • Journal Title: Eur.J.Pharmacol. Volume: 644 Pages: 215-219
  • Peer Reviewed
• [Journal Article] Involvement of multiple PKC isoforms in phorbol 12, 13-dibutyrate-induced contraction during high K+ depolarization in bronchial smooth muscle of mice (2010)
  • Author(s): Hiroyasu Sakai
  • Journal Title: J.Smooth Muscle Res. Volume: 46 Pages: 225-233
  • NAID: 10026983116
  • Peer Reviewed
• [Journal Article] Sphingosine-1-phosphate aggravates antigen-induced airway inflammation in mice. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Open Respir.Med.J. Volume: 4 Pages: 82-85
  • Peer Reviewed
• [Journal Article] SKI-II, an inhibitor of sphingosine kinase, ameliorates antigen-induced bronchial smooth muscle hyperresponsiveness, but not airway inflammation, in mice. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J.Pharmacol.Sci. Volume: 114 Pages: 304-310
  • NAID: 10029890451
  • Peer Reviewed
• [Journal Article] Involvement of Src family kinase activation in angiotensin II-induced hyperresponsiveness of rat bronchial smooth muscle. (2010)
  • Author(s): Hiroyasu Sakai
  • Journal Title: Peptides Volume: 31 Pages: 2216-2221
  • Peer Reviewed
• [Journal Article] Augmented PDBu-mediated contraction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. (2010)
  • Author(s): Hiroyasu Sakai
  • Journal Title: J.Smooth Muscle Res. Volume: 46 Pages: 259-266
  • NAID: 10026983193
  • Peer Reviewed
• [Journal Article] A functional interaction between CPI-17 and RACK-1 proteins in bronchial smooth muscle cells. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 401 Pages: 487-490
  • Peer Reviewed
• [Journal Article] Effects of repeated antigen exposure on the expression of enzymes associated with sphingosine-1-phosphate turnover. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Open Inflamm.J. Volume: 3 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Involvement of K+ channels in the augmented nasal venous responsiveness to nitric oxide in rat model of allergic rhinitis. (2010)
  • Author(s): Hiroyasu Sakai
  • Journal Title: Microvas.Res. Volume: 81 Pages: 129-134
  • Peer Reviewed
• [Journal Article] Phosphorylation of signal transducer and activator of transcription 6 (STAT6) and STAT1, but not STAT3, induced by antigen inhalation in bronchial smooth muscles of sensitized mice. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Biol.Pharm. Bull. 33 Pages: 146-149
  • NAID: 130000140210
  • Peer Reviewed
• [Journal Article] Interleukin-4 upregulates RhoA protein via an activation of STAT6 in cultured human bronchial smooth muscle cells. (2010)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Pharmacol.Res. 61 Pages: 188-192
  • Peer Reviewed
• [Journal Article] Possible involvements of NF-kB and AP-1 in the TNF-a-induced upregulation of MMP-12 in human alveolar epithelial A549 cell line. (2010)
  • Author(s): Yu Yingyan
  • Journal Title: J.Pharmacol.Sci. 112 Pages: 83-88
  • Peer Reviewed
• [Journal Article] The proximal STAT6 and NF-kB sites are responsible for RhoA transcription in human bronchial smooth muscle. (2010)
  • Author(s): Kumiko Goto
  • Journal Title: Pharmacol.Res. 61 Pages: 466-472
  • Peer Reviewed
• [Journal Article] Activation of signal transducer and activator of transcription 1 by interleukin-13 and -4 in cultured human bronchial smooth muscle cells. (2009)
  • Author(s): Chiba, Y., Todoroki, M., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 45 Pages: 279-288
• [Journal Article] GGTI-2133, an inhibitor of geranylgeranyltransferase, inhibits infiltration of inflammatory cells into airways in mouse experimental asthma. (2009)
  • Author(s): Chiba, Y., Sato, S., Misawa, M.
  • Journal Title: Int.J.Immunopathol.Pharmacol. 22 Pages: 929-935
• [Journal Article] Inhibition of geranylgeranyltransferase inhibits bronchial smooth muscle hyperresponsiveness in mice. (2009)
  • Author(s): Chiba, Y., Sato, S., Hanazaki, M., Sakai, H., Misawa, M.
  • Journal Title: Am.J.Physiol.Lung Cell.Mol.Physiol. 297
• [Journal Article] Downregulation of miR-133a contributes to upregulation of RhoA in bronchial smooth muscle cells. (2009)
  • Author(s): Chiba, Y., Tanabe, M., Goto, K., Sakai, H., Misawa, M.
  • Journal Title: Am.J.Respir.Crit.Care Med. 180 Pages: 713-719
• [Journal Article] Some different effect of PKC inhibitors on the acetylcholine-, and endothelin-1-induced contractions of rat bronchial smooth muscle. (2009)
  • Author(s): Sakai, H., Yamamoto, M., Chiba, Y., Misawa, M.
  • Journal Title: Eur.J.Pharmacol. 618 Pages: 58-62
• [Journal Article] TNF-a induces upregulation of RhoA via NF-kB activation in cultured human bronchial smooth muscle cells. (2009)
  • Author(s): Goto, K., Chiba, Y., Sakai, H., Misawa, M.
  • Journal Title: J.Pharmacol.Sci. 110 Pages: 437-444
• [Journal Article] Functional importance of the cytoskeletal protein in acetylcholine-induced contraction of rat bronchial smooth muscle. (2009)
  • Author(s): Sakai, H., Kozutsumi, Y., Goto, K., Chiba, Y., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 45 Pages: 97-108
  • NAID: 10025298888
• [Journal Article] Lovastatin inhibits antigen-induced airway eosinophilia without affecting the production of inflammatory mediators in mice. (2009)
  • Author(s): Chiba, Y., Sato, S., Misawa, M.
  • Journal Title: Inflamm.Res. 58 Pages: 363-369
• [Journal Article] Upregulation of ADAM8 in the airways of mice with allergic bronchial asthma. (2009)
  • Author(s): Chiba, Y., Onoda, S., Hattori, Y., Maitani, Y., Sakai, H., Misawa, M.
  • Journal Title: Lung 187 Pages: 179-185
• [Journal Article] A novel STAT6 inhibitor AS1517499 ameliorates antigen-induced bronchial hypercontractility in mice. (2009)
  • Author(s): Chiba, Y., Todoroki, M., Nishida, Y., Tanabe, M., Misawa, M.
  • Journal Title: Am.J.Respir.Cell Mol.Biol. 41 Pages: 516-524
• [Journal Article] Identification of PKC isoforms expressed in human bronchial smooth muscle cell. (2009)
  • Author(s): Sakai, H., Yamamoto, M., Kozutsumi, Y., Chiba, Y., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 45 Pages: 55-62
  • NAID: 130000860929
• [Journal Article] IL-13 induces translocation of NF-kB in cultured human bronchial smooth muscle cells. (2009)
  • Author(s): Goto, K., Chiba, Y., Misawa, M.
  • Journal Title: Cytokine. 46 Pages: 96-99
• [Journal Article] Interleukin-13 augments bronchial smooth muscle contractility via an upregulation of RhoA protein. (2009)
  • Author(s): Chiba, Y., Nakazawa, S., Todoroki, M., Shinozaki, K., Sakai, H., Misawa, M.
  • Journal Title: Am.J.Respir.Cell Mol.Biol. 40 Pages: 159-167
• [Journal Article] A novel STAT6 inhibitor AS1517499 ameliorates antigen-induced bronchial hypercontractility in mice. (2009)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Am.J.Respir. Cell Mol.Biol. 41 Pages: 516-524
  • Peer Reviewed
• [Journal Article] Upregulation of ADAM8 in the airways of mice with allergic bronchial asthma. (2009)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Lung 187 Pages: 179-185
  • Peer Reviewed
• [Journal Article] Lovastatin inhibits antigen-induced airway eosinophilia without affecting the production of inflammatory mediators in mice. (2009)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Inflam.Res. 58 Pages: 363-369
  • Peer Reviewed
• [Journal Article] Functional importance of the cytoskeletal protein in acetylcholine-induced contraction of rat bronchial smooth muscle. (2009)
  • Author(s): Hiroyasu Sakai
  • Journal Title: J.Smooth Muscle Res. 45 Pages: 97-108
  • NAID: 10025298888
  • Peer Reviewed
• [Journal Article] TNF-α induces upregulation of RhoA via NF-kB activation in cultured human bronchial smooth muscle cells. (2009)
  • Author(s): Kumiki Goto
  • Journal Title: J.Pharmacol.Sci. 110 Pages: 437-444
  • Peer Reviewed
• [Journal Article] Some different effect of PKC inhibitors on the acetylcholine, and endothelin-1-induced contractions of rat bronchial smooth muscle. (2009)
  • Author(s): Hiroyasu Sakai
  • Journal Title: Eur.J.Pharmacol. 618 Pages: 58-62
  • Peer Reviewed
• [Journal Article] Downregulation of miR-133a contributes to upregulation of RhoA in bronchial smooth muscle cells. (2009)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Am.J.Respir.Crit. Care Med. 180 Pages: 713-719
  • Peer Reviewed
• [Journal Article] Inhibition of geranylgeranyltransferase inhibits bronchial smooth muscle hyperresponsiveness in mice. (2009)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Am.J.Physiol. Lung Cell. Mol.Physiol. 297
  • Peer Reviewed
• [Journal Article] GGTI-2133, an inhibitor of geranylgeranyltransferase, inhibits infiltration of inflammatory cells into airways in mouse experimental asthma. (2009)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Int.J.Immunopathol. Pharmacol. 22 Pages: 929-935
  • Peer Reviewed
• [Journal Article] Activation of signal transducer and activator of transcription 1 by interleukin-13 and -4 in cultured human bronchial smooth muscle cells. (2009)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J.Smooth Muscle Res. 45 Pages: 279-288
  • Peer Reviewed
• [Journal Article] Interleukin-13 augments bronchial smooth muscle contractility via an upregulation of RhoA protein. (2009)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Am. J. Respir. Cell Mol. Biol. 40 Pages: 159-167
  • Peer Reviewed
• [Journal Article] IL-13induces translocation of NF-kB in cultured human bronchial smooth muscle cells. Cytokine. 46, 96-99 (2009)
  • Author(s): Kumiko Goto
  • Journal Title: Cytokine 46 Pages: 96-99
  • Peer Reviewed
• [Journal Article] Identification of PKC isofoms expressed in human bronchial smooth muscle cell. J. Smooth Muscle Res. (2009)
  • Author(s): Hiroyasu Sakai
  • Journal Title: J. Smooth Muscle Res. 45 Pages: 55-62
  • Peer Reviewed
• [Journal Article] Y-27632 augments the isoflurane-induced relaxation of bronchial smooth muscle in rats. (2008)
  • Author(s): Hanazaki, M., Chiba, Y., Yokoyama, M., Morita, K., Kohjitani, A., Sakai, H., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 44 Pages: 189-193
  • NAID: 130000860913
• [Journal Article] Inhibition of antigen-induced bronchial smooth muscle hyperresponsiveness by lovastatin in mice. (2008)
  • Author(s): Chiba, Y., Sato, S., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 44 Pages: 123-128
  • NAID: 110006967875
• [Journal Article] Glucocorticoids inhibited airway hyperresponsiveness through downregulation of CPI-17 in bronchial smooth muscle. (2008)
  • Author(s): Goto, K., Chiba, Y., Sakai, H., Misawa, M.
  • Journal Title: Eur.J.Pharmacol. 591 Pages: 231-236
• [Journal Article] Increased expression of Galphaq protein in bronchial smooth muscle of mice with allergic bronchial asthma. (2008)
  • Author(s): Chiba, Y., Shinozaki, K., Ueno, A., Sakai, H., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 44 Pages: 95-100
• [Journal Article] Rho-kinase inhibitors augment the inhibitory effect of propofol on rat bronchial smooth muscle contraction. (2008)
  • Author(s): Hanazaki, M., Yokoyama, M., Morita, K., Kohjitani, A., Sakai, H., Chiba, Y., Misawa, M.
  • Journal Title: Anesth.Analg. 106 Pages: 1765-1771
• [Journal Article] Probable involvement of epsilon-isoform of protein kinase C in rat bronchial smooth muscle contraction induced by acetylcholine. (2008)
  • Author(s): Sakai, H., Yamamoto, M., Chiba, Y., Misawa, M.
  • Journal Title: J.Smooth Muscle Res. 44 Pages: 9-16
  • NAID: 110006664779
• [Journal Article] Glucocorticoids ameliorate antigen-induced bronchial smooth muscle hyperresponsiveness by inhibiting upregulation of RhoA in rats. (2008)
  • Author(s): Chiba, Y., Goto, K., Hirahara, M., Sakai, H., Misawa, M.
  • Journal Title: J.Pharmacol.Sci. 106 Pages: 615-625
  • NAID: 10024320036
• [Journal Article] Lovastatin inhibits bronchial hyperresponsiveness by reducing RhoA signaling in rat allergic asthma. (2008)
  • Author(s): Chiba, Y., Arima, J., Sakai, H., Misawa, M.
  • Journal Title: Am.J.Physiol.Lung Cell.Mol.Physiol. 294
• [Journal Article] Lovastatin inhibits bronchial hyperresponsiveness by reducing RhoA signaling in rat allergic asthma. (2008)
  • Author(s): Yoshihiko Chiba
  • Journal Title: Am. J. Physiol. Lung Cell. Mol. Physiol. 294
  • Peer Reviewed
• [Journal Article] Glucocorticoids ameliorate antigen-induced bronchial smooth muscle hyperresponsiveness by inhibiting upregulation of RhoA in rats. (2008)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J. Pharmacol. Sci. 106 Pages: 615-625
  • NAID: 10024320036
  • Peer Reviewed
• [Journal Article] Probable involvement of epsilon-isoform of protein kinase C in rat bronchial smooth muscle contraction induced by acetylcholine. (2008)
  • Author(s): Hiroyasu Sakai
  • Journal Title: J. Smooth Muscle Res. 44 Pages: 9-16
  • NAID: 110006664779
  • Peer Reviewed
• [Journal Article] Rho-kinase inhibitors augment the inhibitory effect of propofol on rat bronchial smooth muscle contraction. (2008)
  • Author(s): Motohiko Hanazaki
  • Journal Title: Anesth. Analg. 106 Pages: 1765-1771
  • Peer Reviewed
• [Journal Article] Increased expression of Galphaq protein in bronchial smooth muscle of mice with allergic bronchial asthma. (2008)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J. Smooth Muscle Res. 44 Pages: 95-100
  • Peer Reviewed
• [Journal Article] Glucocorticoids inhibited airway hyperresponsiveness through downregulation of CPI-17 in bronchial smooth musele. (2008)
  • Author(s): Kumiko Goto
  • Journal Title: Eur. J. Pharmacol. 591 Pages: 231-236
  • Peer Reviewed
• [Journal Article] Inhibition of antigen-induced bronchial smooth muscle hyperresponsiveness by lovastatin in mice. (2008)
  • Author(s): Yoshihiko Chiba
  • Journal Title: J. Smooth Muscle Res. 44 Pages: 123-128
  • NAID: 110006967875
  • Peer Reviewed
• [Journal Article] Y-27632 augments the isoflurane-induced relaxation of bronchial smooth musele in rats. . (2008)
  • Author(s): Motohiko Hanazaki
  • Journal Title: J. Smooth Muscle Res. 44 Pages: 189-193
  • Peer Reviewed
• [Presentation] ラット気管支平滑筋のangiotensin II誘発気道過敏性におけるp42/44 ERKとSrc family kinaseの相互作用 (2011)
  • Author(s): 酒井寛泰、千葉義彦, ほか
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 横浜(震災のため誌上開催)
• [Presentation] 培養ヒト気管支平滑筋細胞におけるIL-13誘発RhoA発現増加に対するSOCS1の抑制効果 (2011)
  • Author(s): 早船真弘、千葉義彦, ほか
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜(震災のため誌上開催)
• [Presentation] 培養ヒト気管支平滑筋細胞におけるIL-13誘発RhoA発現増加に対するSOCS1の抑制効果 (2011)
  • Author(s): 早船真弘
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜(震災のため紙上開催)
• [Presentation] ラット気管支平滑筋のangiotensin II誘発気道過敏性におけるp42/44 ERKとSrc family kinaseの相互作用 (2011)
  • Author(s): 酒井寛泰
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 横浜(震災のため紙上開催)
• [Presentation] Inhibitory effect of AS1517499, a novel STAT6 inhibitor, on antigen-induced airway hyperresponsiveness in mice. (2010)
  • Author(s): 千葉義彦, et al
  • Organizer: 16th World Congress on Basic and Clinical Pharmacology (WorldPharma2010)
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2010-07-19
• [Presentation] Inhibitory effect of AS1517499, a novel STAT6 inhibitor, on antigen-induced airway hyperresponsiveness in mice (2010)
  • Author(s): Yoshihiko Chiba
  • Organizer: 16th World Congress on Basic and Clinical Pharmacology (World Pharma2010)
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2010-07-19
• [Presentation] ラット抗原誘発気道過敏性時のRhoA upregulationに対するprednisoloneによる抑制メカニズムの解明 (2010)
  • Author(s): 茂俣茉紀子、千葉義彦, ほか
  • Organizer: 第122回日本薬理学会関東部会
  • Place of Presentation: 静岡
  • Year and Date: 2010-06-05
• [Presentation] ラットにおけるRhoA遺伝子プロモーター領域の同定およびその特徴 (2010)
  • Author(s): 千葉義彦
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-30
• [Presentation] 気管支平滑筋におけるヒトRhoA遺伝子プロモーター領域の解析 (2010)
  • Author(s): 後藤久充子、千葉義彦, ほか
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-16
• [Presentation] アレルギー性気管支喘息時の気道過敏性発症におけるmiR-133aの役割 (2010)
  • Author(s): 千葉義彦
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-16
• [Presentation] 抗原誘発過敏性気管支平滑筋におけるRhoAプロモーター活性亢進のメカニズム (2010)
  • Author(s): 後藤久充子
  • Organizer: 第120回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-16
• [Presentation] 気管支平滑筋におけるヒトRhoA遣伝子プロモーター領域の解析 (2010)
  • Author(s): 後藤久充子
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-16
• [Presentation] 抗原誘発過敏性気管支平滑筋におけるRhoAプロモーター活性亢進のメカニズム (2009)
  • Author(s): 後藤久充子、千葉義彦, ほか
  • Organizer: 第120回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-11
• [Presentation] Effects of geranylgeranyltransferase inhibitor- 2133 on antigen-induced airway hyperresponsiveness and inflammation in mice (2009)
  • Author(s): 佐藤駿介、千葉義彦, ほか
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-18
• [Presentation] 培養ヒト気管支平滑筋細胞におけるIL-13誘発抑制性分子およびRhoAタンパク質発現におけるSTAT6の役割 (2009)
  • Author(s): 西田雄一、千葉義彦, ほか
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-18
• [Presentation] 培養ヒト気管支平滑筋細胞におけるIL-13誘発抑制性分子およびRhoAタンパク質発現におけるSTAT6の役割 (2009)
  • Author(s): 西田雄一
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-03-18
• [Presentation] Effects of geranylgeranyltransferase inhibitor-2133 on antigen-induced airway hyperresponsiveness and inflammation in mice. (2009)
  • Author(s): 佐藤駿介
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-03-18
• [Presentation] アレルギー性気管支喘息モデルマウスの気道過敏性および気道炎症発現に及ぼすGGTase阻害薬(GGTI-2133)の効果 (2008)
  • Author(s): 佐藤駿介、千葉義彦, ほか
  • Organizer: 第119回日本薬理学会関東部会
  • Place of Presentation: 川崎
  • Year and Date: 2008-10-04
• [Presentation] マウス抗原誘発気道過敏性時の気管支平滑筋組織における各種SOCS分子種発現の変化 (2008)
  • Author(s): 西田雄一、千葉義彦, ほか
  • Organizer: 第119回日本薬理学会関東部会
  • Place of Presentation: 川崎
  • Year and Date: 2008-10-04
• [Presentation] 培養ヒト気管支平滑筋細胞でのTNF-a/IL-13誘発RhoA発現におけるシグナル伝達の解明 (2008)
  • Author(s): 後藤久美子
  • Organizer: 第119回日本薬理学会関東部会
  • Place of Presentation: 神奈川県川崎市
  • Year and Date: 2008-10-04
• [Presentation] マウス抗原誘発気道過敏性時の気管支平滑筋組織における各種socs分子種発現の変化 (2008)
  • Author(s): 西田雄一
  • Organizer: 第119回日本薬理学会関東部会
  • Place of Presentation: 神奈川県川崎市
  • Year and Date: 2008-10-04
• [Presentation] アレルギー性気管支喘息モデルマウスの気道過敏性および気道長症発現に及ぼすGGTase阻害薬(GGTI-2133)の効果 (2008)
  • Author(s): 佐藤駿介
  • Organizer: 第119回日本薬理学会関東部会
  • Place of Presentation: 神奈川県川崎市
  • Year and Date: 2008-10-04
• [Presentation] 培養ヒト気管支平滑筋細胞におけるSOCS分子種発現に対するIL-13の効果 (2008)
  • Author(s): 西田雄一
  • Organizer: 第118回日本薬理学会関東部会
  • Place of Presentation: 東京都品川区
  • Year and Date: 2008-06-07
• [Presentation] 培養ヒト気管支平滑筋細胞でのRhoAタンパク質発現におけるTNF-a/NF-kB経路およびIL-13/STAT6経路の関与 (2008)
  • Author(s): 後藤久美子
  • Organizer: 第118回日本薬理学会関東部会
  • Place of Presentation: 東京都品川区
  • Year and Date: 2008-06-07
• [Presentation] マウス喘息時の気道過敏性・気道炎症に対するlovastatinの効果 (2008)
  • Author(s): 佐藤駿介
  • Organizer: 第118回日本薬理学会関東部会
  • Place of Presentation: 東京都品川区
  • Year and Date: 2008-06-07
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