Study on effects of Helicobacter pylori infection on expression and function of proteinase-activated receptors in gastric mucosal epithelium.

• Project/Area Number: 20590097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kinki University
• Principal Investigator: SEKIGUCHI Fumiko Kinki University, 薬学部, 准教授 (90271410)
• Co-Investigator(Kenkyū-buntansha): KAWABATA Atsufumi 近畿大学, 薬学部, 教授 (20177728)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 薬理学 / 受容体発現 / 感染症 / 細胞・組織 / 細菌 / 癌 / Proteinase-activated receptor / ヘリコバクター・ピロリ / 炎症

Research Abstract

In the cancer area of gastric mucosal epithelium from gastric cancer patients, we found a tendency that expression of proteinase-activated receptor-1 (PAR1) was positively correlated with infection levels of Helicobacter pylori (H. pylori), suggesting involvement of increased expression of PAR1 in the development of H. pylori-related gastric cancer. We also determined if H. pylori might possess PAR-activating proteinases in various cell lines. However, we did not find any proteinases that activate PAR1, PAR2 or PAR4, in the H. pylori extracts.

Research Products

• [Journal Article] Biological activity of Helicobacter pylori components in mammalian cells : is it independent of proteinase-activated receptors? (2012)
  • Author(s): SEKIGUCHI, F, MATSUMOTO, Y, MAEDA, Y, TSUBOTA-MATSUNAMI, M, NISHIKAWA, H, and KAWABATA, A.
  • Journal Title: Journal of Physiology and Pharmacology. Volume: 63 Pages: 571-576
  • Peer Reviewed
• [Presentation] Analysis of various biological responses induced by Helicobacter pylori extract : independent of proteinase-activated receptors? (2010)
  • Author(s): Sekiguchi F, et al.
  • Organizer: 16th World Congress on Basic and Clinical Pharmacology
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2010-07-20
• [Presentation] Helicobacter pylori 抽出液により誘起される種々の細胞反応: Proteinase-activated receptor関与の有無について (2009)
  • Author(s): 関口富美子,前田優磨,松本裕喜,松波真帆,西川裕之,川畑篤史
  • Organizer: 第116回 日本薬理学会近畿部会
  • Place of Presentation: 滋賀(口頭発表)
  • Year and Date: 2009-11-13
• [Presentation] Helicobacter pylori 抽出液により誘起される種々の細胞反応:Proteinase-activated receptor 関与の有無について (2009)
  • Author(s): 関口富美子, 他
  • Organizer: 第116回日本薬理学会近畿部会
  • Place of Presentation: 大津
  • Year and Date: 2009-11-13
• [Presentation] Effect of Helicobacter pylori extracts on prostaglandin E_2 production in human lung alveolar epithelial A549 cells. XXXVI (2009)
  • Author(s): Maeda Y, Sekiguchi R. Matsumoto Y. Nishikawa H. Kawabata A.
  • Organizer: International Congress of Physiological Sciences
  • Place of Presentation: Kyoto(ポスター発表)
  • Year and Date: 2009-08-01
• [Presentation] Effect of Helicobacter pylori extracts on prostaglandin E_2 production in human lung alveolar epithelial A549 cells (2009)
  • Author(s): Maeda Y, Sekiguchi F, et al.
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-27
• [Presentation] Relationship between expression of PARS and infection with H. pylori in the stomach of gastric cancer patients. (2009)
  • Author(s): FkmiKmi K., Sekiguchi, F., Takaoka, K Miyake K Yamamoto T., Tanaka S., Ohno K., Ishikura H., Naruse M., Kawabata A.
  • Organizer: Experimental Biology 2009
  • Place of Presentation: New Orleans(ポスター発表)
  • Year and Date: 2009-04-21
• [Presentation] Relationship between expression of PARs and infection with H.pylori in the stomach of gastric cancer patients (2009)
  • Author(s): Fukami A, Sekiguchi F, et al.
  • Organizer: Experimental Biology 2009
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2009-04-21
• [Presentation] 胃癌患者より摘出した胃組織のヘリコバクター・ピロリ由来ウレアーゼ蛋白量とPAR1あるいはPAR2発現量の相関性について (2009)
  • Author(s): 関口富美子,深海和樹,高岡香保理,三宅建作,山本隆嗣,田中肖吾,大野耕一,石倉宏恭,成瀬光栄,川畑篤史
  • Organizer: 第82回 日本薬理学会年会
  • Place of Presentation: 横浜(ポスター発表)
  • Year and Date: 2009-03-16
• [Presentation] 胃癌患者より摘出した胃組織のヘリコバクター・ピロリ由来ウレアーゼ蛋白量とPAR1あるいはPAR2発現量の相関性について (2009)
  • Author(s): 関口富美子
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
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