| Project/Area Number |
20590111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Osaka University of Pharmaceutical Sciences |
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Principal Investigator |
ISHIDA Toshimasa Osaka University of Pharmaceutical Sciences, 薬学部, 教授 (00111021)
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| Co-Investigator(Kenkyū-buntansha) |
TOMOO Koji 大阪薬科大学, 薬学部, 准教授 (70257898)
IN Yasuko 大阪薬科大学, 薬学部, 講師 (50257896)
MINOURA Katsuhiko 大阪薬科大学, 薬学部, 講師 (10278591)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 医薬分子設計 / タウ蛋白質 / 微小管結合ドメイン / 重合抑制 / 阻害剤 / 抗体 / 抑制機構 / 構造解析 / C-H…Π結合 / 食品 |
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| Research Abstract |
(1) The microtubule-binding domain (MBD) of tau protein is the region responsible for the PHF formation. We succeed in the preparation of an antibody Tau2r3, which selectively recognizes the MBD. The X-ray crystal structure analysis of the complex crystal of its variable region, Fab2r3, with the VQIINK fragment peptide of MBD clarified the overall complex structure. Now, we are investigating the recognition mode of MBD by the antibody at the atomic level. (2) The MBD interaction of a series of inhibitors we discovered so far were investigated by 1H-NMR and other physicochemical methods, and clarified the structural elements necessary for exhibiting the inhibitory activity, i. e., inhibitor has a planar aromatic ring including the polar atom (O or N) or polar group (NH or OH) on the ring. On the other hand, the PHF formation experiment of a series of tau mutants clarified the important of CH…π interaction between Ile308 and Tyr310 residues in the third repeat of MBD.
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