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Analysis of multidrug resistance systems in multidrug resistant bacteria and development of drugs effective on the resistant bacteria

Research Project

Project/Area Number 20590120
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionOkayama University

Principal Investigator

TSUCHIYA Tomofusa  Okayama University, 大学院・医歯薬学総合研究科, 教授 (80012673)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords多剤耐性菌 / 耐性系 / 多剤排出ポンプ / 医薬品開発 / 抗菌薬 / バイオフィルム形成阻害 / MRSA / VRE / 抗菌物質 / バイオフィルム阻害物質 / 耐性菌 / 抗MRSA作用
Research Abstract

The worst 1 of cause of human death is infectious disease by pathogenic microorganisms. In developed countries, such as our country and countries in Europe and North America, infectious diseases by multidrug-resistant bacteria are serious problems. Many drugs used at clinical sites are not effective on the multidrug resistant bacteria. Aims of this research are to analyze multidrug resistant systems in multidrug resistant bacteria and to develop drugs effective on such resistant bacteria. In fact, we analyzed multidrug resistant systems in several multidrug resistant bacteria, and found several seed compounds effective on such multidrug resistant bacteria.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (34 results)

All 2011 2010 2009 2008

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (14 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Patch Clamp Analysis of the Respiratory Chain in Bacillus subtilis2011

    • Author(s)
      Nakamura K.
    • Journal Title

      Biochim.Biophys.Acta

      Volume: 1808 Pages: 1103-1107

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design, synthesis, and biological evaluation of a novel series of quercetin diacylglucosides as potent anti-MRSA and anti-VRE agents.2010

    • Author(s)
      Hossion, A.M., N.Otsuka, R.K.Kandahary, T.Tsuchiya, W.Ogawa, A.Iwado, Y.Zamami, K.Sasaki.
    • Journal Title

      Bioorg.Med.Chem.Lett. 20

      Pages: 5349-5352

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Design, synthesis and biological evaluation of a novel series of quercetin diacylglucosides as a potent anti-MRSA and anti-VRE agents.2010

    • Author(s)
      Hossion A.M.
    • Journal Title

      Bioorg.Med.Cehm.

      Volume: 20 Pages: 5349-5352

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synergistic effect of kaempferol glycosides purified from Laurus nobilis and fluoroquinolones on methicillin-resistant Staphylococcus aureus.2009

    • Author(s)
      Liu, M.H., N.Otsuka, K.Noyori, S.Shiota, W.Ogawa, T.Kuroda, T.Hatano, T.Tsuchiya.
    • Journal Title

      Biol.Pharm.Bull. 32

      Pages: 489-492

    • NAID

      110007122695

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and characterization of EfmA, a multidrug efflux pump, from Enterococcus faecium.2009

    • Author(s)
      Nishioka, T., W.Ogawa, T.Kuroda, T.Katsu, T.Tsuchiya.
    • Journal Title

      Biol.Pharm.Bull. 32

      Pages: 483-488

    • NAID

      110007122697

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa.2009

    • Author(s)
      Mima, T., N.Kohira, Y.Li, H.Sekiya, W.Ogawa, T.Kuroda, T.Tsuchiya.
    • Journal Title

      Microbiology 155

      Pages: 3509-3517

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of natural polyphenols on methicillin-resistant Staphylococcus aureus in relation to their chemical and physical properties.2009

    • Author(s)
      Hatano, T., M.Kusuda, M.Tsugawa, S.Taniguchi, T.Tsuchiya, T.Yoshida.
    • Journal Title

      Phytochem.Res.

      Pages: 31-47

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multidrug efflux transporters in the MATEfamily2009

    • Author(s)
      Teruo Kuroda
    • Journal Title

      Biochim. Biophys. Acta 1794

      Pages: 763-768

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of Vibrio mimicus hemolysin through limited proteolysis by an endogenous metalloprotease2009

    • Author(s)
      Tamaki Mizuno
    • Journal Title

      FEBS J. 276

      Pages: 825-834

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa.2009

    • Author(s)
      Takehiko Mima
    • Journal Title

      Microbiology 155

      Pages: 3509-3517

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synergistic effect of kaempferol glvcosides purified from Laurus nobilis and fluoroquinolones on methicillin-resistant Staphylococcus aureus2009

    • Author(s)
      Mei-Hua Liu
    • Journal Title

      Biol. Pharm. Bull. 32

      Pages: 489-492

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and characterization of EfrA, a multidrug efflux pump, from Enterococcus faecium2009

    • Author(s)
      Toshihiro Nishioka
    • Journal Title

      Biol. Pharm. Bull. 32

      Pages: 483-488

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens.2008

    • Author(s)
      Minato, Y., F.Shahcheraghi, W.Ogawa, T.Kuroda, T.Tsuchiya.
    • Journal Title

      Biol.Pharm.Bull. 31

      Pages: 516-519

    • NAID

      110006623085

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of antibacterial effects of epigallocatechin gallate, using ascorbic acid.2008

    • Author(s)
      Hatano, T., M.Tsugawa, M.Kusuda, S.Taniguchi, T.Yoshida, S.Shiota, T.Tsuchiya.
    • Journal Title

      Phytochemistry 69

      Pages: 3111-3116

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-methicillin resistant Staphylococcus aureus (MRSA) compounds isolated from Laurus nobilis.2008

    • Author(s)
      Otsuka, N., M.H.Liu, S.Shiota, W.Ogawa, T.Kuroda, T.Hatano, T.Tsuchiya.
    • Journal Title

      Biol.Pharm.Bull. 31

      Pages: 1794-1797

    • NAID

      110006878971

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens2008

    • Author(s)
      Yusuke Minato
    • Journal Title

      Biol. Pharm, Bull. 31

      Pages: 516-519

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-methicillin resistant Staphylococcus aureus (MRSA)compounds isolated from Laurus nobilis2008

    • Author(s)
      Nao Otsuka
    • Journal Title

      Biol. Pharra. Bull. 31

      Pages: 1794-1797

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of antibacterial effects of epigallocatechin gallate, using ascorbic acid2008

    • Author(s)
      Tsutomu Hatano
    • Journal Title

      Phytochemistry 69

      Pages: 3111-3116

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 多剤耐性緑膿菌の分離及びその耐性系の解析2011

    • Author(s)
      保田菜美, 他
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Serattia marcescens消毒薬耐性変異株の分離と耐性系の解析2011

    • Author(s)
      芳賀仁美, 他
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Staphylococcus aureusの多剤排出ポンプ破壊株の作製と多剤肺s h通ポンプの網羅的解析2011

    • Author(s)
      森田大地, 他
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Serratia marcescens消毒薬耐性変異株の分離と耐性系の解析2011

    • Author(s)
      芳賀仁美
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡市
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 黄色ブドウ球菌の付着因子およびバイオフィルム形成に対する各種テルペノイドの影響2009

    • Author(s)
      塩田澄子, 他
    • Place of Presentation
      東京
    • Year and Date
      2009-06-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 各種抗菌物質と緑膿菌の多剤耐性化の関係2009

    • Author(s)
      藤田 智子
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 抗MRSA効果をもっ月桂耐由来kaempferol配糖体の作用点の解析2009

    • Author(s)
      大塚 菜緒
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 黄色ブドウ球菌の多剤排出ポンプNorAに対するアントラキノン系物質の阻害効果2009

    • Author(s)
      吉野 秀紀
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 緑膿菌の多剤排出ポンプ遺伝子emrEの発現制御におけるEmrTの役割2009

    • Author(s)
      小平 尚輝
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-03-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肺炎桿菌の抗菌薬耐性変異株の分離2009

    • Author(s)
      Rui-Ting Ni
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-03-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肺炎桿菌のRND型多剤排出ポンプKexDの解析2009

    • Author(s)
      小川 和加野
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-03-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 枯草菌多剤耐陛変異株における多剤排出ポンプ遺伝子yheIHの発現上昇2009

    • Author(s)
      西岡 俊博
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-03-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] MRSAの多剤高度耐性化における耐性因子の役割2008

    • Author(s)
      宅野 正浩
    • Organizer
      日本薬学会中国四国支部大会
    • Place of Presentation
      岡山市
    • Year and Date
      2008-11-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肺炎球菌のMATE型多剤排出ポンプPdrMの解析2008

    • Author(s)
      橋本 康平
    • Organizer
      日本細菌学会中国四国支部総会
    • Place of Presentation
      松山市
    • Year and Date
      2008-10-18
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] バイオフィルム形成阻害剤2010

    • Inventor(s)
      土屋友房、塩田澄子
    • Industrial Property Rights Holder
      土屋友房、塩田澄子
    • Filing Date
      2010-05-04
    • Related Report
      2010 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] バイオフィルム形成阻害剤2009

    • Inventor(s)
      土屋友房、塩田澄子
    • Industrial Property Rights Holder
      土屋友房、塩田澄子
    • Filing Date
      2009-04-13
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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