Molecular- and structural-biological studies on plant necrosis

Research Project

Project/Area Number	20590123
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Environmental pharmacy
Research Institution	Kyushu University
Principal Investigator	MORIMOTO Satoshi Kyushu University, 大学院・薬学研究院, 教授 (60191045)
Co-Investigator(Kenkyū-buntansha)	TAMADA Taro 日本原子力研究開発機構, 研究主幹 (50391248) TAURA Futoshi 九州大学, 大学院・薬学研究院, 助教 (00301341)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	薬用資源学 / ネクローシス / カンナビノイド / ミトコンドリア / モノクローナル抗体 / Cyclophilin D / VDAC / THCA
Research Abstract	Two cannabinoids were identified as plant necrosis-inducing factors from Cannabis sativa. In addition, genes encoding cyclophilin D (CYD) and voltage-dependent anion channel (VDAC), which are assumed to be involved in necrosis, were cloned and expressed in E. coli. Anti-VDAC antibodies were prepared using recombinant VDAC. Animal VDAC antibodies are known to inhibit necrosis in animal cells, whereas the above VDAC antibodies obtained in my study had such inhibitory effect for plant cells.