Programmed cell death in neuronal cells induced by a trienoic fatty acid
| Project/Area Number |
20590132
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
KONDO Kazunari National Institute of Health Sciences, 代謝生化学部, 主任研究官 (40270623)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 脂肪酸 / アポトーシス / 神経科学 / きのこ / 共役トリエン脂肪酸 / AIF / PARP / ERK/MEK / スギヒラタケ / ERK / MEK / MAPK |
|---|
| Research Abstract |
In order to clarify the mechanisms by which eleostearic acid (ESA) induces neuronal cell death, cell viability assay was performed using various inhibitors and RNA interference for apoptosis-related molecules such as MEK, PARP-1, AIE ESA induces apoptotic cell death morphologically. However, activation of caspase and PARP-1, and DNA damage were not observed. AIF migrates from mitochondria to the nucleus upon ESA stimulation. ERK is also phosphorylated by ESA. The cell death is blocked by the inhibition of MEK and antioxidant tocopherol.
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Report
(4 results)
Research Products
(24 results)
