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Studies on biosynthesis and metabolism of the glutathione conjugated bile acids

Research Project

Project/Area Number 20590164
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionKinki University

Principal Investigator

MITAMURA Kuniko  Kinki University, 薬学部, 准教授 (70242526)

Co-Investigator(Kenkyū-buntansha) IKEGAWA Shigeo  近畿大学, 薬学部, 教授 (90111301)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords胆汁酸 / グルタチオン / N-アセチルシステイン / エレクトロスプレーイオン化 / 質量分析法 / 胆汁 / プロドラッグ / 硫酸抱合 / LC-MS / 活性代謝物 / ラット / グルタチオン抱合体 / N-アセチルシスティン抱合体 / 尿 / カルボキシルエステラーゼ
Research Abstract

In order to elucidate the mechanism on the formation and metabolism of glutathione (GSH) conjugated bile acids via their reactive intermediates, we demonstrated that bile acid were tansacylated with GSH and N-acetylcysteine (NAC) by in vitro and in vivo studies. In addition, we clarified that the several kinds of glutathione conjugated bile acids were excreted into the bile of rats and patients with biliary diseases. We also showed the NAC conjugated ursodeoxycholic acid could be a prodrug for hepatobiliary fuction improvement drug.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (111 results)

All 2011 2010 2009 2008

All Journal Article (32 results) (of which Peer Reviewed: 27 results) Presentation (76 results) Book (3 results)

  • [Journal Article] Identification of bile acid S-acyl glutathione conjugates in rat bile by liquid chromatography/electro- spray ionization-linear ion trap mass spectrometry2011

    • Author(s)
      Mitamura K, Hori N, Iida T, Hofmann AF, Ikegawa S
    • Journal Title

      Steroids 76

      Pages: 68-77

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous demmaination of tetrahydrocorticosteroid glucuronide by liquid chromatography/elearospray i onization-mass spectrometry2011

    • Author(s)
      Mitamura K., et al.
    • Journal Title

      Korean J.Lab.Med.

      Volume: 30

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Identification of bile acid S-acyl glutathione conjugates in rat bile by liquid chromatography,/electispray ionization-linear ion trap mass spectrometry.2011

    • Author(s)
      Mitamura K., et al.
    • Journal Title

      Steroids

      Volume: 76 Pages: 68-77

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous determination of tetrahydrocortico-steroid glucuronide by liquid chromato-graphy/electrospray ionization-mass spectrometry2010

    • Author(s)
      三田村邦子,池川繁男
    • Journal Title

      Korean J.Lab.Med. 30(Suppl.2)

    • Related Report
      2010 Final Research Report
  • [Journal Article] Salivary cheno-deoxycholic acid and its glycine-conjugate : Their determination method using LC-MS/MS and variation of their concentrations with increased saliva flow rate2010

    • Author(s)
      Higashi T, Shibayama Y. Ichikawa T, Ito K, Toyo'oka T, Shimada K: Mitamura K, Ikegawa S, Chiba H
    • Journal Title

      Steroids 75

      Pages: 338-345

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential corticoid metabolites : chemical synthesis of 3-and 21-mono-and 3,21-disulfate conjugates of tetrahydrocorticosteroids in the 5α- and 5β series2010

    • Author(s)
      Okihara R, Mitamura K, Hasegawa M, Mori M, Muto A, Kakiyama G, Iida T, Shimada M, Mano N, Ikegawa S
    • Journal Title

      Chem.Pharm.Bull. 58

      Pages: 344-353

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential Corticoid Metabolites : Chemical Synthesis of 3-and 21-Mono- and 3,21-Disulfate Conjugates of Tetrahydroconicosteroids in the 5α- and 5β-Series.2010

    • Author(s)
      Okihara R, et al.
    • Journal Title

      Chem.Pharm.Bull.

      Volume: 58 Pages: 344-353

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salivary chenodeoxycholic acid and its glvcine-conjugate: Their determination method using LC-MS/MS and validation of their concentration with increased saliva flow rate.2010

    • Author(s)
      Higashi T., et al
    • Journal Title

      Steroids

      Volume: 75 Pages: 338-345

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential Corticoid Metabolites : Chemical Synthesis of 3- and 21-Mono- and 3,21-Disulfate Conjugates of Tetrahydrocorticosteroids in the 5α- and 5β-Series2010

    • Author(s)
      Okihara R., et al.
    • Journal Title

      Chem.Pharm.Bull. 58

      Pages: 344-353

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salivary chenodeoxycholic acid and its glycine-conjugate : Their determination method using LC-MS/MS and validation of their concentration with increased saliva flow rate2010

    • Author(s)
      Higashi T., et al.
    • Journal Title

      Steroids 75

      Pages: 338-345

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous determination of twelve tetrahydrocorticosteroid glucuronide in human urine by liquid chromatography/ electrospray ionization iinear ion trap mass spectrometry2009

    • Author(s)
      Ikegawa S, Hasegawa M, Okihara R, Shimizu C, Chiba H, Iida T, Mitamura K
    • Journal Title

      Anal.Chem. 81

      Pages: 10124-10135

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 胆汁酸のグルタチオン抱合体とLC/MS2009

    • Author(s)
      三田村邦子,堺俊博,池川繁男,若宮建昭
    • Journal Title

      JUSCO Report 51

      Pages: 7-13

    • NAID

      40016867163

    • Related Report
      2010 Final Research Report
  • [Journal Article] LC/ESI-MSによる内分泌・代謝疾患の科学診断へのアプローチ2009

    • Author(s)
      三田村邦子,長谷川真紀,沖原梨香,池川繁男
    • Journal Title

      臨床化学 38

      Pages: 291-298

    • NAID

      10025633137

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemical synthesis of N- acetylcysteine conjugates of bile acids and in vivo formation in cholestatic rats as shown by liquid chromatography/ electrospray ionization -linear ion trap mass spectrometry2009

    • Author(s)
      Mitamura K, Watanabe S, Sakai T, Okihara R, Sogabe M, Wakamiya T, Hofmann AF, Ikegawa S
    • Journal Title

      J.Chromatogr.B. 877

      Pages: 2630-2638

    • NAID

      10028022525

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemical Synthesis of bile acid acyl-adenylates and formation by rat liver microsomal fraction2009

    • Author(s)
      Ikegawa S, Ito H, Ohshima M, Maeda M, Hofmann AF, Mitamura K
    • Journal Title

      Steroids 74

      Pages: 751-757

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatog-raphy/electrospray ionization-linear ion trap mass spectrometry2009

    • Author(s)
      Mitamura K, Watanabe S, Mitsumoto Y, Sakai T, Sogabe M, Wakamiya T, Ikegawa S
    • Journal Title

      Anal.Biochem. 384

      Pages: 224-230

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hofmann AF, Iida T : A facile Synthesis of C-24 and C-25 oxysterols by in situ generated ethyl(trifluoromethyl)dioxirane2009

    • Author(s)
      Ogawa S, Kakiyama G, Muto A, Hosoda A, Mitamura K, Ikegawa S
    • Journal Title

      Steroids 74

      Pages: 81-87

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry2009

    • Author(s)
      三田村邦子 K., et al.
    • Journal Title

      Anal.Biochem. 384

      Pages: 224-230

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical synthesis of N-acetylsysteine conjugates of bile acids and in vivo formation in cholestatic rats as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry2009

    • Author(s)
      三田村邦子 K., et al.
    • Journal Title

      J.Chromatogr.B 877

      Pages: 2630-2638

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous Determination of Twelve Tetrahydrocorticosteroid Glucuronide in Human Urine by Liquid Chromatography/Electrospray Ionization Linear Ion Trap Mass Spectrometry2009

    • Author(s)
      Ikegawa S., et al.
    • Journal Title

      Anal.Chem. 81

      Pages: 10124-10135

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical Synthesis of bile acid acyl-adenylates and formation by rat liver microsomal fraction2009

    • Author(s)
      Ikegawa S., et al.
    • Journal Title

      Steroids 74

      Pages: 751-757

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LC/ESI-MSによる内分泌・代謝疾患の科学診断へのアプローチ2009

    • Author(s)
      三田村邦子, et al.
    • Journal Title

      臨床化学 38

      Pages: 291-298

    • NAID

      10025633137

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 胆汁酸のグルタチオン抱合とLC/MS2009

    • Author(s)
      三田村邦子, et al.
    • Journal Title

      Jasco Report 51

      Pages: 7-13

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      池川繁男
    • Journal Title

      薬学分析科学の最前線(じほう)

      Pages: 130-131

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry2009

    • Author(s)
      Mitamura K., et. al
    • Journal Title

      Anal. Biochem. 384

      Pages: 224-230

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production and characterization of monoclonal antibody for capture of proteins tagged with lithocholic acid2008

    • Author(s)
      Ikegawa S, Yamamoto T, Miyashita T, Okihara R, Ishiwata S, Sakai T, Chong R-H., Maeda M, Hofmann AF, Mitamura K
    • Journal Title

      Anal.Sci. 24

      Pages: 1475-1480

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunoprecipitation and MALDI-MS identification of lithocholic acid- tagged proteins in liver of bile duct-ligated rats2008

    • Author(s)
      Ikegawa S, Yamamoto T, Ito H, Ishiwata S, Sakai T, Mitamura K, Maeda M
    • Journal Title

      J.Lipid Res. 49

      Pages: 2463-2473

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] コレステロール硫酸転位酵素とコレステロール代謝2008

    • Author(s)
      布田博敏,三田村邦子,池川繁男
    • Journal Title

      臨床化学 37

      Pages: 148-160

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] コレステロール硫酸転位酵素とコレステロール代謝2008

    • Author(s)
      布田博敏, ら
    • Journal Title

      臨床化学 37

      Pages: 148-160

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunoprecipitation and MALDI-MS identification of litho-cholic acid-tagged proteins in liver of bile duct-ligated rats2008

    • Author(s)
      Ikegawa S., et. al.
    • Journal Title

      J. Lipid Res. 49

      Pages: 2463-2473

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production and characterization of monoclonal antibody for capture of proteins tagged with lithocholic acid2008

    • Author(s)
      Ikegawa S., et. al.
    • Journal Title

      Anal. Sci. 24

      Pages: 1475-1480

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A facil Synthesis of C-24 and C-25 oxysterols by in situ generated ethyl (trifluoromethyl) dioxirane2008

    • Author(s)
      Ogawa S., et al.
    • Journal Title

      Steroids 74

      Pages: 81-87

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] LC/ESI-MS/MSによるヒト胆汁中グルタチオン抱合型胆汁酸の同定2011

    • Author(s)
      三田村邦子
    • Organizer
      第21回日本臨床化学会近畿支部総会
    • Place of Presentation
      大阪
    • Year and Date
      2011-02-05
    • Related Report
      2010 Final Research Report
  • [Presentation] LCIMSによるヒト胆汁中グルタチオン抱合型胆汁酸の同定2011

    • Author(s)
      三田村邦子
    • Organizer
      第21回日本臨床化学会近畿支部総会
    • Place of Presentation
      大阪
    • Year and Date
      2011-02-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるヒト胆汁中グルタチオン抱合型胆汁酸の同定2011

    • Author(s)
      池川繁男
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] グルタチオン抱合型胆汁酸3-サルフェートの合成とラット肝サイトゾール画分における生成2011

    • Author(s)
      三田村邦子
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] グルタチオン抱合型胆汁酸のラット肝サイトゾール画分における硫酸抱合の解析2010

    • Author(s)
      池川繁男
    • Organizer
      第32回胆汁酸研究会
    • Place of Presentation
      仙台
    • Year and Date
      2010-11-09
    • Related Report
      2010 Final Research Report
  • [Presentation] グルタチオン抱合型胆汁酸のラット肝サイトゾール画分における硫酸抱合の解析2010

    • Author(s)
      池川繁男
    • Organizer
      第32回月酬酸研究会
    • Place of Presentation
      仙台
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 胆汁酸のグルタチオン抱合体と胆汁排泄2010

    • Author(s)
      池川繁男
    • Organizer
      第27回日本胆膵病態・生理研究会
    • Place of Presentation
      弘前
    • Year and Date
      2010-06-26
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Mass Spectrometric Techniques Applied to the Study of Conjugated Bile Acids, Steroids, and Oxysterols.2010

    • Author(s)
      池川繁男
    • Organizer
      Research Seminar at Virginia Commonwealth Uni- versity, Medical College of Virginia Campus
    • Place of Presentation
      Richmond, USA(招待講演)
    • Year and Date
      2010-05-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 重水素標識N-アセチルシステインによる胆汁酸代謝活性中間体のトラッピング2010

    • Author(s)
      池川繁男
    • Organizer
      第19回日本小児胆汁酸研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-02-20
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 肝・胆・消化機能改善作用を指向したプロドラッグ開発の基礎的検討:重水素標識N-アセチルシステイン抱合型ウルソデオキシコール酸の体内動態の追跡2010

    • Author(s)
      三田村邦子
    • Organizer
      第19回日本小児胆汁酸研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-02-20
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Analysis of glutathione conjugates of bile acids in rat bile by LC/ESI-MS/MS. XXI International Bile Acid Meeting.2010

    • Author(s)
      池川繁男
    • Organizer
      Bile Acid as Metabolic Integrators and Therapeutics
    • Place of Presentation
      Freiburg, Germany
    • Related Report
      2010 Final Research Report
  • [Presentation] Chemical synthesis of the (25R)- and (25S)-epimers of 3α,7α,12α-trihydroxy-5α-cholestan-27-oic acid and their glycine and taurine conjugates. XXI International Bile Acid Meeting.2010

    • Author(s)
      Iida T
    • Organizer
      Bile Acid as Metabolic Integrators and Therapeutics
    • Place of Presentation
      Freiburg, Germany
    • Related Report
      2010 Final Research Report
  • [Presentation] Simultaneous determination of tetrahydrocorticosteroid glucuronides by liquid chromatography/electrospray ioniza-tion-mass spectrometry.2010

    • Author(s)
      三田村邦子
    • Organizer
      The 12th Asian-Pacific Congress of Clinical Biochemistry(シンポジウム)
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒト血中硫酸抱合型オキシステロールの同位体希釈LC/ESI-MSによる直接一斉分析法の開発2010

    • Author(s)
      坂野理絵
    • Organizer
      第50回日本臨床化学会年次学術集会
    • Place of Presentation
      甲府
    • Related Report
      2010 Final Research Report
  • [Presentation] イオンクラスター法による胆汁酸代謝活性中間体のチオール基並びにアミノ基との反応性の解析2010

    • Author(s)
      三田村邦子
    • Organizer
      第35回日本医用マススペクトル学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] 内分泌・代謝疾患のLC/MSによるメタボロミクスに用いる多重重水素標識A/B-シス-テトラヒドロコルチコステロイド・サルフェートの合成2010

    • Author(s)
      池川繁男
    • Organizer
      第23回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      松島
    • Related Report
      2010 Final Research Report
  • [Presentation] Quantitative metabolic profiling of twelve tetrahyrocorticosteroid glucur- onides in urine by isotope dilution LC-ESI- linear ion trap MS/MS.2010

    • Author(s)
      三田村邦子
    • Organizer
      58th ASMS Conference on Mass Spectrometry
    • Place of Presentation
      Salt Lake City, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of novel metabolites of bile acids : S-acyl glutathione conjugates in rat bile by LC/ESI-linear ion trap MS/MS.2010

    • Author(s)
      Hori N
    • Organizer
      58th ASMS Conference on Mass Spectrometry
    • Place of Presentation
      Salt Lake City, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] ラット胆汁中グルタチオン抱合型胆汁酸の合成とLC/MSによる同定2010

    • Author(s)
      堀直宏
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるラット胆汁中重水素標識N-アセチルシステイン抱合型胆汁酸の同定2010

    • Author(s)
      堺俊博
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるヒト尿中テトラヒドロ-並びにヘキサヒドロコルチコステロイド・グルクロニド直接一斉分析法の開発2010

    • Author(s)
      三田村邦子
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2010 Final Research Report
  • [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型ウルソデオキシコール酸の体内動態に関する基礎的検討2010

    • Author(s)
      池川繁男
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Quantitative Metabolic Profiling of Twelve Tenahyroconicosteroid Glucuronides in Unne by Isotope Dilution LC-BST-Linear Ion Trap MS/MS.2010

    • Author(s)
      Mitamura K.
    • Organizer
      58th ASMS Conference on Mass Spectrometry
    • Place of Presentation
      ソルトレイクシティー・米国
    • Related Report
      2010 Annual Research Report
  • [Presentation] IdentiScation of Novel Metabolites of Bile Acids : S-Acyl Glutathione Conjugates in Rat Bile by LC,'EST -Linear Ton Trap MS/MS.2010

    • Author(s)
      Hori N.
    • Organizer
      58th ASMS Conference on Mass Spectrometry
    • Place of Presentation
      ソルトレイクシティー・米国
    • Related Report
      2010 Annual Research Report
  • [Presentation] 内分泌・代謝疾患のLC/MSによるメタボロミクスに用いる多重重水素標識AIB-シス-テトラヒドロコルチコステロイド・サルフェートの合成2010

    • Author(s)
      池川繁男
    • Organizer
      第23回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      松島
    • Related Report
      2010 Annual Research Report
  • [Presentation] イオンクラスター法による胆汁酸代謝活性中間体のチオール基並びにアミの基との反応性の解析2010

    • Author(s)
      三田村邦子
    • Organizer
      第35回日本医用マススベクトル学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト血中硫酸抱合型オキシステロールの同位体希釈LC/ESK-MSによる直接斉分析法の開発2010

    • Author(s)
      坂野理絵
    • Organizer
      第50回日本臨床化学会年次学術集会
    • Place of Presentation
      甲府
    • Related Report
      2010 Annual Research Report
  • [Presentation] Simultaneous Determination of Tetrahydrocorticosteroid Glucuronides by Liquid Chromatogra phy/Electrospray Ionization-Mass Spectrometry2010

    • Author(s)
      Mitamura K.
    • Organizer
      The 12th Asian-Pacific Congress of Clinical Biochcmistry
    • Place of Presentation
      ソウル・韓国
    • Related Report
      2010 Annual Research Report
  • [Presentation] Chemical synthesis of the (25R)-and (25S)-epimers of 3a,7a,12a-trihydroxy-5a-cholestan-27-oic acid and their glycinc and taurine conjugates.2010

    • Author(s)
      Iida T.
    • Organizer
      XXI International Bile Acid Meeting Bile Acid as Metabolic Integrators and Therapeutics
    • Place of Presentation
      フライブルク・ドイツ
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of glutathione conjugates of bile acids in rat bile by LC/ESI-MS/MS.2010

    • Author(s)
      Ikegawa S.
    • Organizer
      XXI International Bile Acid Meeting Bile Acid as Metabolic Integrators and Therapeutics
    • Place of Presentation
      フライブルク・ドイツ
    • Related Report
      2010 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるヒト尿中テトラヒドロ-並びにヘキサヒドロコルチコステロイド・クルクロニド直接一斉分析法の開発2010

    • Author(s)
      三田村邦子
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Related Report
      2009 Annual Research Report
  • [Presentation] 肝・胆・消化機能改善薬として期待されるN-アセチルシステイン抱合型ウルソデオキシコール酸の体内動態に関する基礎的研究2009

    • Author(s)
      池川繁男
    • Organizer
      第20回日本臨床化学会近畿支部総会
    • Place of Presentation
      大阪
    • Year and Date
      2009-12-26
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 質量分析(LC/MS)と臨床化学2009

    • Author(s)
      池川繁男
    • Organizer
      第27回日本臨床化学会関東支部例会
    • Place of Presentation
      東京(依頼講演)
    • Year and Date
      2009-12-05
    • Related Report
      2010 Final Research Report
  • [Presentation] ラット胆汁中グルタチオン抱合体の合成とこれらのLC/MSによる同定2009

    • Author(s)
      堀直宏
    • Organizer
      第31回胆汁酸研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-11-07
    • Related Report
      2010 Final Research Report
  • [Presentation] ラット胆汁中グルタチオン抱合体の合成とこれらのLC/MSによる同定2009

    • Author(s)
      堀直宏
    • Organizer
      第31回胆汁酸研究会
    • Place of Presentation
      大阪
    • Year and Date
      2009-11-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多重重水素標識テトラヒドロコルチコステロイド・サルフェートの合成とESI-MSnにおける挙動2009

    • Author(s)
      三田村邦子
    • Organizer
      第59回日本薬学会近畿支部大会
    • Place of Presentation
      大阪
    • Year and Date
      2009-10-24
    • Related Report
      2010 Final Research Report
  • [Presentation] LC/MSによる硫酸抱合型オキシステロール直接一斉分析法の開発2009

    • Author(s)
      坂野理絵
    • Organizer
      第59回日本薬学会近畿支部大会
    • Place of Presentation
      大阪
    • Year and Date
      2009-10-24
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 多重重水素標識テトラヒドロコルチコステロイド・サルフェートの合成とESI-Ms^nにおける挙動2009

    • Author(s)
      三田村邦子
    • Organizer
      第59回日本薬学会近畿支部大会
    • Place of Presentation
      大阪
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるラット並びにヒト胆汁中グルタチオン抱合型胆汁酸の解析2009

    • Author(s)
      三田村邦子
    • Organizer
      第19回西日本臨床胆汁酸研究会
    • Place of Presentation
      大阪
    • Year and Date
      2009-07-25
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] ラット胆汁中アミノ酸並びにグルタチオン抱合型胆汁酸の解析2009

    • Author(s)
      堺俊博
    • Organizer
      第18回日本小児胆汁酸研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-02-21
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] テトラヒドロコルチコステロイドのグルクロン酸抱合に関する基礎的検討2009

    • Author(s)
      三田村邦子
    • Organizer
      第19回日本臨床化学会近畿支部総会
    • Place of Presentation
      大阪
    • Year and Date
      2009-01-24
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Identification of glutathione conjugates of bile acids in rat bile by LC/ESI-MS2.2009

    • Author(s)
      池川繁男
    • Organizer
      日本薬物動態学会第24回年会
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型胆汁酸3-サルフェートのトレースアナリシス2009

    • Author(s)
      池川繁男
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] 同位体希釈LC/ESI-MS/MSによる尿中テトラヒドロコルチコステロイド・サルフェート定量法の開発2009

    • Author(s)
      三田村邦子
    • Organizer
      第49回日本臨床化学会年会
    • Place of Presentation
      長崎
    • Related Report
      2010 Final Research Report
  • [Presentation] 唾液中胆汁酸:LC-ESI-MS/MS定量法と唾液分泌量亢進に伴う濃度変動2009

    • Author(s)
      東達也
    • Organizer
      第34回日本医用マススペクトル学会年会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型胆汁酸のラット肝サイトゾール画分における硫酸抱合の解析2009

    • Author(s)
      堺俊博
    • Organizer
      第34回日本医用マススペクトル学会年会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] ラット胆汁中グルタチオン抱合型胆汁酸の同定2009

    • Author(s)
      堀直宏
    • Organizer
      第34回日本医用マススペクトル学会年会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 胆汁酸のグルタチオン抱合と胆汁排泄2009

    • Author(s)
      池川繁男
    • Organizer
      第22回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      岐阜
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] LC/MSによる臨床化学へのアプローチ2009

    • Author(s)
      三田村邦子
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都(シンポジウム)
    • Related Report
      2010 Final Research Report
  • [Presentation] LC/MSによる硫酸抱合型オキシステロールの高感度定量法の開発2009

    • Author(s)
      三田村邦子
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] A/B-trans構造を有するテトラヒドロコルチコステロイド・サルフェートの合成とESI-MSにおける挙動2009

    • Author(s)
      沖原梨香
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるラット胆汁中グルタチオン抱合型胆汁酸の解析2009

    • Author(s)
      堺俊博
    • Organizer
      フィジカル・ファーマフォーラム2009
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] 同位体希釈LC/ESI-MS/MSによる尿中テトラヒドロコルチコステロイド・サルフェート定量法の開発2009

    • Author(s)
      三田村邦子
    • Organizer
      第49回日本臨床化学会年次学術集会
    • Place of Presentation
      長崎
    • Related Report
      2009 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるN-アセチルシステイン抱合型胆汁酸3-サルフェートのトレースアナリシス2009

    • Author(s)
      池川繁男
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      札幌
    • Related Report
      2009 Annual Research Report
  • [Presentation] N-アセチルシステインとの抱合体形成による胆汁酸の新規代謝経路の解明に関する研究2009

    • Author(s)
      中井莉沙
    • Organizer
      第46回ペプチド討論会
    • Place of Presentation
      飯塚
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of Glutathione Conjugates of Bile Acids in Rat Bile by LC/ESI-MS^22009

    • Author(s)
      池川繁男
    • Organizer
      日本薬物動態学会第24回年会
    • Place of Presentation
      京都
    • Related Report
      2009 Annual Research Report
  • [Presentation] LC/ESI-MS/MSによるラット胆汁中グルタチオン抱合型胆汁酸の解析2009

    • Author(s)
      堺俊博
    • Organizer
      フィジカル・フアーマフォーラム2009
    • Place of Presentation
      大阪
    • Related Report
      2008 Annual Research Report
  • [Presentation] LC/MSによる臨床化学へのアプローチ2009

    • Author(s)
      三田村邦子
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Related Report
      2008 Annual Research Report
  • [Presentation] 胆汁酸のN-アセチルシステイン抱合に関する基礎的研究2008

    • Author(s)
      三田村邦子
    • Organizer
      第30回胆汁酸研究会
    • Place of Presentation
      広島
    • Year and Date
      2008-10-18
    • Related Report
      2010 Final Research Report
  • [Presentation] In situ発生ジオキシランを用いるコレスタン側鎖C-25位の直接水酸化とオキシステロール類の単段階合成2008

    • Author(s)
      柿山玄太
    • Organizer
      第30回胆汁酸研究会
    • Place of Presentation
      広島
    • Year and Date
      2008-10-18
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 胆汁酸のN-アセチルシスティン抱合に関する基礎的研究2008

    • Author(s)
      三田村邦子
    • Organizer
      第30回胆汁酸研究会
    • Place of Presentation
      広島
    • Year and Date
      2008-10-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] A new insight in the formation of N-acetylcysteine conjugates of bile acids.2008

    • Author(s)
      池川繁男
    • Organizer
      日本薬物動態学会第23回年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] LC/MSによる胆汁酸のグルタチオン抱合に関する研究2008

    • Author(s)
      三田村邦子
    • Organizer
      第33回日本医用マススペクトル学会年会
    • Place of Presentation
      東京(奨励賞受賞講演)
    • Related Report
      2010 Final Research Report
  • [Presentation] N-アセチルシステイン抱合型胆汁酸3-サルフェートの負イオン検出エレクトロスプレーイオン化-タンデムマススペクトロメトリーにおける挙動2008

    • Author(s)
      堺俊博
    • Organizer
      第33回日本医用マススペクトル学会年会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] 液体クロマトグラフィー/質量分析法による内分泌代謝疾患の科学的診断へのアプローチ2008

    • Author(s)
      三田村邦子
    • Organizer
      第48回日本臨床化学会年次学術集会
    • Place of Presentation
      浜松(学術賞受賞講演)
    • Related Report
      2010 Final Research Report
  • [Presentation] LC/ESI-MSnによる尿中硫酸抱合型テトラヒドロコルチコステロイドの直接一斉分析2008

    • Author(s)
      沖原梨香
    • Organizer
      第48回日本臨床化学会年次学術集会
    • Place of Presentation
      浜松
    • Related Report
      2010 Final Research Report
  • [Presentation] LC/MSによる胆汁酸の体内動態解析に基づくプロドラッグの開発2008

    • Author(s)
      三田村邦子
    • Organizer
      第21回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] Mechanistic studies on the formation of acyl glutathione conjugate of bile acid in rat liver and its biliary excretion.2008

    • Author(s)
      三田村邦子
    • Organizer
      Falk Symposium 165. XX International Bile Acid Meeting, Bile Acid Biology and Therapeutic Actions
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of lithocholic acid-tagged proteins in the rat liver by using immnoaffinity capture and MALDI-TOF.2008

    • Author(s)
      池川繁男
    • Organizer
      Falk Symposium 165. XX International Bile Acid Meeting, Bile Acid Biology and Therapeutic Actions
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of lithocholic acid-tagged proteins in the rat liver by using immnoaffinity capture and MALDI-TOF2008

    • Author(s)
      Ikegawa S.
    • Organizer
      Bile Acid Meeting, Bile Acid Biology and Therapeutic Actions
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2008 Annual Research Report
  • [Presentation] Mechanistic studies on the formation of acyl glutathione conjugate of bile acid in rat liver and its biliary excretion2008

    • Author(s)
      Mitamura K.
    • Organizer
      Bile Acid Meeting, Bile Acid Biology and Therapeutic Actions
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2008 Annual Research Report
  • [Presentation] LC/ESI-MS^nによる胆汁酸の体内動態解析に基づくプロドラッグの開発2008

    • Author(s)
      三田村邦子
    • Organizer
      第21回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2008 Annual Research Report
  • [Presentation] LC/ESI-MS^nによる尿中硫酸抱合型テトラヒドロコルチコステロイドの直接一斉分析2008

    • Author(s)
      沖原梨香
    • Organizer
      第48回日本臨床化学会年次学術集会
    • Place of Presentation
      浜松
    • Related Report
      2008 Annual Research Report
  • [Presentation] 液体クロマトグラフィー/質量分析法による内分泌代謝疾患の化学的診断へのアプローチ2008

    • Author(s)
      三田村邦子
    • Organizer
      第48回日本臨床化学会年次学術集会
    • Place of Presentation
      浜松
    • Related Report
      2008 Annual Research Report
  • [Presentation] N-アセチルシスティン抱合型胆汁酸3-サルフェートの負イオン検出エレクトロスプレーイオン化-タンデムマススペクトロメトリーにおける挙動2008

    • Author(s)
      堺俊博
    • Organizer
      第33回日本医用マススペクトル学会年会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] LC/MSによる胆汁酸のグルタチオン抱合に関する研究2008

    • Author(s)
      三田村邦子
    • Organizer
      第33回日本医用マススペクトル学会年会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Book] 薬学生のための臨床化学2010

    • Author(s)
      池川繁男,三田村邦子
    • Publisher
      南江堂
    • Related Report
      2010 Final Research Report
  • [Book] 薬学生のための臨床化学(改訂第3版)2010

    • Author(s)
      池川繁男
    • Publisher
      南江堂
    • Related Report
      2010 Annual Research Report
  • [Book] 薬学分析科学の最前線2009

    • Author(s)
      池川繁男,三田村邦子, じほう
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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