Molecular basis for the region specific morphogenesis of the rib depending on the interaction between somites and surface ectoderm.

• Project/Area Number: 20590171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Hiroshima University
• Principal Investigator: AOYAMA Hirohiko 広島大学, 大学院・医歯薬学総合研究科, 教授 (70143948)
• Co-Investigator(Renkei-kenkyūsha): MATSUI Kouji 広島大学, 大学院・医歯薬学総合研究科, 助教 (80183945) ; SAKAMOTO Nobuyuki 広島大学, 大学院・医歯薬学総合研究科, 助教 (90346487)
• Research Collaborator: YAMAGUCHI Mami 大阪大学, 大学院・理学研究科, 大学院生 ; ISABELLA Kurnia liem 広島大学, 大学院・医歯薬学総合研究科, 大学院生 ; MASUDA Tasuku 広島大学, 大学院・医歯薬学総合研究科, 大学院生 ; MAEDA Kazutaka 広島大学, 大学院・医歯薬学総合研究科, 大学院生 ; FAN Haiming 広島大学, 大学院・医歯薬学総合研究科, 大学院生
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 形態形成 / 体節 / 骨格 / 脊椎動物 / ボディプラン / 発生・分化 / 進化 / ニワトリ胚 / 組織間相互作用 / 中軸骨格 / 解剖学 / 発現制御

Research Abstract

In the course of evolution, the vertebrae bearing rib localized to form thorax in birds and mammals. The rib is constituted of three developmental compartments. The most distal compartment is the sternal rib, which was not formed when contact between the somites and the lateral plate mesoderm was interrupted in a two-day chick embryo. In this case, the abaxial somitic cells did not appeared. Labeling each somite individually, the 27th somite was shown to form almost entire abdominal muscles as well as the most caudal rib, suggesting that the lumbar somites form neither the ribs nor the body wall muscles. To understand molecular entity of the interaction between surface ectoderm and somites in the distal rib-genesis, Wnt6 and Fzd7 were cloned.

Research Products

• [Journal Article] Body wall morphogenesis : limb-genesis interferes with body wall-genesis via its in fluence on the abaxial somite derivatives (2010)
  • Author(s): Liem, I. K., Aoyama, H.
  • Journal Title: Mech Dev Volume: 126 Pages: 198-211
• [Journal Article] Elevated intraocular pressure, optic nerve atrophy, and impaired retinal development in ODAG transgenic mice. Invest. (2009)
  • Author(s): Sasaki, T., Watanabe, W., Muranishi, Y., Kanamoto, T., Aihara, M., Miyazaki, K., Tamura, H., Saeki, T., Oda, H., Souchelnytskyi, N., Souchelnytskyi, S., Aoyama, H., Honda, Z., Furukawa, T., Mishima, H. K., Kiuchi, Y., Honda, H.
  • Journal Title: Ophthalmol. Vis. Sci Volume: 50 Pages: 242-248
• [Journal Article] Body wall morphogenesis : limb-genesis interferes with body wall-genesis via its influence on the abaxial somite derivatives (2009)
  • Author(s): Liem, I.K., Aoyama, H.
  • Journal Title: Mech Dev. 126 Pages: 198-211
  • Peer Reviewed
• [Journal Article] Elevated intraocular pressure, optic nerve atrophy, and impaired retinal development in ODAG transgenic mice. (2009)
  • Author(s): Sasaki, T., Watanabe, W., Muranishi, Y., Kanamoto, T., Aihara, M., Miyazaki, K., Tamura, H., Saeki, T., Oda, H., Souchelnytskyi, N., Souchelnytskyi, S., Aoyama, H., Honda, Z., Furukawa, T., Mishima, H. K., Kiuchi, Y., Honda, H.
  • Journal Title: Invest. Ophthalmol. Vis. Sci. 50 Pages: 242-248
  • Peer Reviewed
• [Journal Article] Body wall morphogenesis : limb-genesis interferes with body wall-genesis via its influence on the abaxial somite derivatives. Body wall morphogenesis : limb-genesis interferes with body wall-genesis via its influence on the abaxial somite derivatives. (2009)
  • Author(s): Liem, l. K., Aoyama, H.
  • Journal Title: Mech. Dev. 126 Pages: 198-211
  • Peer Reviewed
• [Presentation] Interaction between the somite cells and the lateral plate mesoderm on the development of ribs : microsurgery on the cell migrating course (2011)
  • Author(s): Haiming Fan
  • Organizer: The 88^th annual meeting of the physiological society of Japan and the 116^th annual meeting of the japanese association of anatomists
  • Place of Presentation: The Journal of Physiological Sciences
• [Presentation] Homology between the thoracic vertebrae with ribs and the cervical/ lumbosacral vertebrae shown by chondrogenesis and ossification pattern (2011)
  • Author(s): Tasuku Masuda, Nobuyuki Sakamoto, Hirohiko Aoyama
  • Organizer: The 88^th annual meeting of the physiological society of Japan and the 116^th annual meeting of the japanese association of anatomists
  • Place of Presentation: The Journal of Physiological Sciences
• [Presentation] he origin and the migration route of the avian somitic abdominal wall muscles (2011)
  • Author(s): Kazutaka Maeda, Nobuyuki Sakamoto, Hirohiko Aoyama
  • Organizer: The 88^th annual meeting of the physiological society of Japan and the 116^th annual meeting of the japanese association of anatomists
  • Place of Presentation: The Journal of Physiological Sciences
• [Presentation] Interaction between the somite cells and the lateral plate mesoderm on the development of ribs : microsurgery on the cell migrating course. (2011)
  • Author(s): Haiming Fan
  • Organizer: The 88^<th> annual meeting of the physiological society of Japan and the 116^<th> annual meeting of the Japanese association of anatomists
  • Place of Presentation: The Journal of Physiological Sciences, 61, suppl.1, 2011, S137.東日本大震災による誌上開催
• [Presentation] Homology between the thoracic vertebrae with ribs and the cervical/lumbosacral vertebrae shown by chondrogenesis and ossification pattern. (2011)
  • Author(s): Tasuku Masuda, Nobuyuki Sakamoto, Hirohiko Aoyama
  • Organizer: The 88^<th> annual meeting of the physiological society of Japan and the 116^<th> annual meeting of the Japanese association of anatomists
  • Place of Presentation: The Journal of Physiological Sciences, 61, suppl.1, 2011, S138.東日本大震災による誌上開催
• [Presentation] The origin and the migration route of the avian somitic abdominal wall muscles. (2011)
  • Author(s): Kazutaka Maeda, Nobuyuki Sakamoto, Hirohiko Aoyama
  • Organizer: The 88^<th> annual meeting of the physiological society of Japan and the 116^<th> annual meeting of the Japanese association of anatomists
  • Place of Presentation: The Journal of Physiological Sciences, 61, suppl.1, 2011, S144.東日本大震災による誌上開催
• [Presentation] 鳥類腹壁筋の由来体節とその移動経路 (2010)
  • Author(s): 前田和孝, 坂本信之, 青山裕彦
  • Organizer: 日仏生物学会第173回例会
  • Place of Presentation: 京都市上京区
  • Year and Date: 2010-12-04
• [Presentation] 脊椎動物の部域特異的体壁形成における体節細胞の移動と組織間相互作用 (2010)
  • Author(s): 范海明, 青山裕彦
  • Organizer: 日本解剖学会第65回中国・四国支部学術集会
  • Year and Date: 2010-10-09
• [Presentation] 部域ごとの椎骨の発生的軟骨・骨形成と肋骨相同部位の同定 (2010)
  • Author(s): 増田佑, 坂本信之, 青山裕彦
  • Organizer: 日本解剖学会第65回中国・四国支部学術集会
  • Year and Date: 2010-10-09
• [Presentation] 腹壁筋の由来体節とその移動経路 (2010)
  • Author(s): 前田和孝, 坂本信之, 青山裕彦
  • Organizer: 日本解剖学会第65回中国・四国支部学術集会
  • Year and Date: 2010-10-09
• [Presentation] 椎骨と肋骨の部域間相同性と部域特異的形態形成 (2010)
  • Author(s): 増田佑, 坂本信之, 青山裕彦
  • Organizer: 日仏生物学会第172回例会
  • Place of Presentation: 東京都渋谷区
  • Year and Date: 2010-05-29
• [Presentation] 頚椎・腰仙椎における肋骨相同部位の同定.軟骨形成,骨化点と肋椎関節形成の解析による (2010)
  • Author(s): 増田佑, 坂本信之, 青山裕彦
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] 頚椎・腰仙椎における肋骨相同部位の同定―軟骨形成, 骨化点と肋椎関節形成の解析による (2010)
  • Author(s): 増田佑, 坂本信之, 青山裕彦
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-03-28
• [Presentation] 筋の発生由来分節と脊髄神経支配 (2009)
  • Author(s): 松井浩二, 山口真未, 青山裕彦
  • Organizer: 日仏生物学会第170回例会
  • Place of Presentation: 東京
  • Year and Date: 2009-06-13
• [Presentation] 腹壁筋の由来体節と神経支配.発生学懇話会シンポジウム「肉眼解剖学と発生生物学の対話」(オーガナイザー;横山尚彦,青山裕彦) (2009)
  • Author(s): 松井浩二, Isabella Kurnia Liem, 山口真未, 青山裕彦
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-30
• [Presentation] 実験形態学と発生生物学.シンポジウム「解剖学を築いた人々」(オーガナイザー;坂井建雄,島田和幸) (2009)
  • Author(s): 青山裕彦
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-30
• [Presentation] 実験形態学と発生生物学 (2009)
  • Author(s): 青山裕彦
  • Organizer: シンポジウム「解剖学を築いた人々」(オーガナイザー ; 坂井建雄, 島田和幸)第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-03-30
• [Presentation] 腹壁筋の由来体節と神経支配 (2009)
  • Author(s): 松井浩二, Isabella Kurnia Liem, 山口真未, 青山裕彦
  • Organizer: 発生学懇話会シンポジウム「肉眼解剖学と発生生物学の対話」(オーガナイザー ; 横山尚彦, 青山裕彦)第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-03-28
• [Presentation] Body wall morphogenesis : The new primaxial-abaxial patterning domains and its functional relevance (2008)
  • Author(s): Liem, I. K., Aoyama, H.
  • Organizer: 12^th Indonesian Association of Anatomists Congress
  • Place of Presentation: Jakarta, Indonesia
  • Year and Date: 2008-06-20
• [Presentation] Regionalization of the vertebrate body wall : A hypothesis (2008)
  • Author(s): Liem, I. K., Yamaguchi, M., Aoyama, H.
  • Organizer: 12^th Indonesian Association of Anatomists Congress
  • Place of Presentation: Jakarta, Indonesia
  • Year and Date: 2008-06-20
• [Presentation] Shoulder and pelvic girdles homology (2008)
  • Author(s): Liem, I. K., Yamaguchi, M., Aoyama, H.
  • Organizer: 12^th Indonesian Association of Anatomists Congress
  • Place of Presentation: Jakarta, Indonesia
  • Year and Date: 2008-06-20
• [Presentation] From somite into the axial skeeton. Experimental morphology in an eggshell (2008)
  • Author(s): Aoyama, H.
  • Organizer: Plenary session, 12^th Indonesian Association of Anatomists Congress
  • Place of Presentation: Jakarta, Indonesia
  • Year and Date: 2008-06-20
• [Presentation] Body wall morphogenesis : The new primaxiai-abaxial patterning domains and its functional relevance. (2008)
  • Author(s): Liem, I. K., Aoyama, H.
  • Organizer: 12^<th> Indonesian Association of Anatomists Congress
  • Place of Presentation: Jakarta, Indonesia
  • Year and Date: 2008-06-20
• [Presentation] Regionalization of the vertebrate body wall : A hypothesis. (2008)
  • Author(s): Liem, I. K., Yamaguchi, M., Aoyama, H.
  • Organizer: 12^<th> Indonesian Association of Anatomists Congress
  • Place of Presentation: Jakarta, Indonesia
  • Year and Date: 2008-06-20
• [Presentation] Shoulder and pelvic girdles homology. (2008)
  • Author(s): Liem, I. K., Yamaguchi, M., Aoyama, H.
  • Organizer: 12^<th> Indonesian Association of Anatomists Congress
  • Place of Presentation: Jakarta, Indonesia
  • Year and Date: 2008-06-20
• [Presentation] From somite into the axial skeeton-Experimental morphology in an eggshell. (2008)
  • Author(s): Aoyama, H.
  • Organizer: Plenary session, 12^<th> Indonesian Association of Anatomists Congress
  • Place of Presentation: Jakarta, Indonesia
  • Year and Date: 2008-06-20
• [Book] 臨床のための解剖学 (2008)
  • Author(s): 青山裕彦
  • Publisher: 坂井建雄監訳