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Trial to identify membranous protein(s) regulating the planar cell polarity

Research Project

Project/Area Number 20590172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionEhime University

Principal Investigator

KOBAYASHI Naoto  Ehime University, 大学院・医学系研究科, 教授 (50234836)

Co-Investigator(Kenkyū-buntansha) HAMADA Fumihiko  大分大学, 医学部, 教授 (70252707)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords発生・分化 / 平面内細胞極性 / 平面内細胞極性形成シグナル伝達 / Frizzled受容体 / Frizzledリガンド / ショウジョウバエS2細胞株 / キメラ受容体分子 / 平面内細胞極性(PCP) / PCP形成シグナル伝達 / Planar Cell Polarity(PCP) / PCP形成シグナル伝達経路 / リガンド
Research Abstract

Planar cell polarity is known to be established mainly by the signals originated from the Frizzled-1 receptor localized on the plasma membrane, although available information on the ligand molecule for Frizzled-1 is still limited. The present study has aimed to reveal the unknown ligand molecule(s) using Drosophila cell culture system coupled with chimeric molecule (Frizzled-1-Frizzled-2 chimeras) technique. We have established culture cell-based assay system of the target molecules, together with the constructs enabling to express various membranous receptor molecules reported in Drosophila. We are trying to survey receptor molecules to reveal the Frizzled-1-ligand in question.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (23 results)

All 2011 2010 2009 2008 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (4 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity.2011

    • Author(s)
      Zhang X-J, Li C, Gao H-L, Nabeka H, Shimokawa T, Wakisaka H, Matsuda S, Kobayashi N
    • Journal Title

      Cellular and Molecular Biology Letters 162

      Pages: 279-295

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity.2011

    • Author(s)
      Zhang, X-J., Li, C., Gao, H-L., Nabeka, H., Shimokawa, T., Wakisaka, H., Matsuda, S., Kobayashi, N.
    • Journal Title

      Cellular and Molecular Biology Letters

      Volume: 162 Pages: 279-295

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sunspot, a link between Wingless signaling and endoreplication in Drosophila.2010

    • Author(s)
      Taniue K, Nishida A, Hamada F , Sugie A, Oda T, Ui-Tei K, Tabata T, Akiyama T
    • Journal Title

      Development 137

      Pages: 1755-1764

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphological maturation level of the esophagus is associated with the number of circumesophageal muscle fibers during archenteron formation in the starfish Patiria (Asterina) pectinifera.2010

    • Author(s)
      Miguchi Y, Takata H, Doihara T, Miyawaki K, Shimokawa T, Hamada F, Kobayashi N, Matsuda S
    • Journal Title

      Biological Bulletin 219

      Pages: 12-16

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphological maturation level of the esophagus is associated with the number of circumesophageal muscle fibers during archenteron formation in the starfish Patiria (Asterina) pectinifera2010

    • Author(s)
      Miguchi, Y., Takata, H., Doihara, T., Miyawaki, K., Shimokawa, T., Hamada, F., Kobayashi, N., Matsuda, S.
    • Journal Title

      Biological Bulletin

      Volume: 219 Pages: 12-16

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sunspot, a link between Wingless signaling and endoreplication in Drosophila2010

    • Author(s)
      Taniue, K., Nishida, A., Hamada, F., Sugie, A., Oda, T., Ui-Tei, K., Tabata, T., Akiyama, T.
    • Journal Title

      Development

      Volume: 137 Pages: 1755-1764

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporal distribution patterns of oligosaccharides during early embryogen esis in the starfish Patiria pectinifera.2009

    • Author(s)
      Doihara T, Miguchi Y, Miyawaki K, Shimokawa T, Hamada F, Kobayashi N, Matsuda S.
    • Journal Title

      Development Genes Evolution 219

      Pages: 199-206

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporal distribution patterns of oligosaccharides during early embryogenesis in the starfish Patiria pectinifera2009

    • Author(s)
      Doihara T, Miguchi Y, Miyawaki K, Shimokawa T, Hamada F, Kobayashi N, Matsuda S
    • Journal Title

      Development Genes Evolution 219

      Pages: 199-206

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains.2008

    • Author(s)
      Tran H, Hamada F, Schwarz-Romond T, Bienz M
    • Journal Title

      Genes and Development 22

      Pages: 528-542

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increase of integrin- linked kinase activity in cultured podocytes upon stimulation with plasma from patients with recurrent FSGS.2008

    • Author(s)
      Hattori M, Akioka Y, Chikamoto H, Kobayashi N, Tsuchiya K, Shimizu M, Kagami S, Tsukaguchi H
    • Journal Title

      American Journal of Transplantation 8

      Pages: 1550-1556

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection.2008

    • Author(s)
      Chen J, Saito S, Kobayashi N, Sato K, Terashita T, Shimokawa T, Mominoki K, Miyawaki K, Sano A, Matsuda S
    • Journal Title

      Neuroscience Research 60

      Pages: 82-94

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains.2008

    • Author(s)
      Tran, H., Hamada, F., Schwarz-Romond, T., Bienz, M.
    • Journal Title

      Genes and Development 22

      Pages: 528-542

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increase of integrin-linked kinase activity in cultured podocytes uponstimulation with plasma from patients with recurrent FSGS.2008

    • Author(s)
      Hattori, M., Kobayashi. N., 他(8名中4人目)
    • Journal Title

      American Journal of Transplantation 8

      Pages: 1550-1556

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection.2008

    • Author(s)
      Chen, J., Kobayashi, N., 他(10名中3人目)
    • Journal Title

      Neuroscience Research 60

      Pages: 82-94

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] カイニン酸神経細胞障害によるプロサポシン動態2008

    • Author(s)
      武智浩子, 上松敬吾, 濱田文彦, 下川哲哉, 小林直人, 松田正司
    • Journal Title

      愛媛医学 27

      Pages: 120-126

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Wntシグナル伝達経路を制御する新規ubiquitin ligaseの機能解析2009

    • Author(s)
      濱田文彦、鍋加浩明、H.Tran, M.Bienz, 小林直人、松田正司
    • Organizer
      日本解剖学会・第65回九州支部学術集会
    • Place of Presentation
      那覇
    • Year and Date
      2009-11-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Wntシグナル伝達経路を制御する新規 ubiquitin ligase の機能解析2009

    • Author(s)
      濱田文彦, 鍋加浩明, H.Tran, M.Bienz, 小林直人, 松田正司
    • Organizer
      日本解剖学会・第65回九州支部学術集会
    • Place of Presentation
      那覇
    • Year and Date
      2009-11-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] Wntシグナル伝達経路を制御する新規ubiquitin ligaseの機能解析2009

    • Author(s)
      鍋加浩明、濱田文彦、宮脇恭史、下川哲哉、小林直人、松田正司
    • Organizer
      日本解剖学会・全国学術集会
    • Place of Presentation
      岡山
    • Year and Date
      2009-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Wntシグナル伝達経路を制御する新規ubiquitin ligaseの機能解析2008

    • Author(s)
      濱田文彦、鍋加浩明、H.Tran, M.Bienz, 小林直人、松田正司
    • Organizer
      日本解剖学会総会・全国集会
    • Place of Presentation
      大分
    • Year and Date
      2008-03-28
    • Related Report
      2010 Final Research Report
  • [Book] Dendritic Spines : Biochemistry, Modeling and Properties "The Structure of Dendritic Spines"2009

    • Author(s)
      Matsuda, Hamada, F, Ishizuka
    • Total Pages
      12
    • Publisher
      NOVA Science publisher
    • Related Report
      2009 Annual Research Report
  • [Book] 細胞・培地活用ハンドブック 「32章 昆虫細胞」2008

    • Author(s)
      濱田文彦(分担執筆), 秋山徹, 河府和義編
    • Publisher
      羊土社
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://kenqweb.office.ehime-u.ac.jp/Profiles/0008/0000359/profile.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://kenqweb.office.ehime-u.ac.jp/Profiles/0008/0000359/profile.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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