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Analysis of functional significance of lipids in receptors for vision, hearing and olfaction by way of functional analysis of fatty acid binding proteins

Research Project

Project/Area Number 20590180
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionTohoku Bunka Gakuen University

Principal Investigator

SAINO Sachiko  Tohoku Bunka Gakuen University, 医療福祉学部, 客員教授 (50312559)

Co-Investigator(Kenkyū-buntansha) 近藤 尚武  東北文化学園大学, 医療福祉学部, 教授 (20004723)
Co-Investigator(Renkei-kenkyūsha) 大和田 祐二  山口大学, 大学院・医学系研究科, 教授 (20292211)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords脂肪酸結合蛋白 / 網膜 / 内耳 / パイエル板 / 免疫組織化学 / 有毛細胞 / 支持細胞 / 聴覚検査 / 遺伝子ノックアウト / 腸パイエル板 / 樹状細胞 / マクロファージ / M-細胞 / ミクログリア / マウス / 網膜介在細胞 / 錐体型視細胞 / 分子多様性局在
Research Abstract

In the retina of normal adult mice, B-FABP was mainly localized in the cone photoreceptor cells, H-FABP in some populations of amacrine/bipolar/horizontal interneurons, and E-FABP in ganglion cells, suggesting the discrete functional involvement of FABPs and their ligands, FAs in the photoreception and photo-transmission. In addition, A-FABP-like immunoreactivity was located in resident microglia of normal retinae. In damaged retinae following photic injury, E-FABP was intensely localized in invasive macrophages, allowing discrete identification of the resident microglia and invasive macrophages by A- and E-FABP immunoreactivity, respectively. In the inner ear of normal adult mice, H (heart- type) -FABP was localized in inner and outer pillar cells and outer phalangeal cells, while B (brain-type)-FABP was localized in border cells and cells of Hensen, and fibrocytes in the spiral limbus and spiral prominence. However, no hair cells expressed any species of FABPs. Althoug the FABP species and/or their ligands, FAs, is suggested to play important roles in the regulation of the hearing function, mutant mice with deletion of the gene for either H- or B-FABP did not show any impairment of the hearing ability. In addition to the two sensory organs, E-FABP was expressed/localized in most, if not all, populations of the dendritic cells in the subepithelial domes, follicles and interfollicular regions of Peyer's patches and presumptive macrophages in their germinal centers, and all M cells in the follicle-associated epithelium of mouse intestine. The immunoreactivity in both of the cell populations makes it easy to recognize the accumulation of DCs in the subepithelial domes in close proximity to the base of M cells, which is essential for luminal antigens to be transported to Peyer's patches. E-FABP may play some important roles in the mucosal immune reaction through Peyer's patches and associated structures.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (13 results)

All 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 5 results) Remarks (1 results)

  • [Journal Article]2011

    • Author(s)
      Ogawa E, 他11名, Kondo H
    • Journal Title

      J Invest Dermatol 131(3)

      Pages: 604-612

    • Related Report
      2010 Final Research Report
  • [Journal Article]2010

    • Author(s)
      Saino-Saito S, Suzuki R, Tokuda N, Abe H, Kondo H, Owada Y
    • Journal Title

      Ann Anat 192(4)

      Pages: 210-214

    • Related Report
      2010 Final Research Report
  • [Journal Article]2010

    • Author(s)
      Tokuda N, 他7名, Kondo H
    • Journal Title

      Histochem Cell Biol 134(5)

      Pages: 445-452

    • Related Report
      2010 Final Research Report
  • [Journal Article] Localization of fatty acid binding protein(FABPs) in the cochlea of mice2010

    • Author(s)
      Saino-Saito S, Suzuki R, Tokuda N, Abe H, Kondo H, Owada Y
    • Journal Title

      Annals Anatomy

      Volume: 192(4) Pages: 210-214

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of FABP7 in fibroblastic reticular cells of mouse lymph nodes2010

    • Author(s)
      Tokuda N, Kondo H, Owada Y, 他6名
    • Journal Title

      Histochem Cell Biol

      Volume: 134(5) Pages: 445-452

    • Related Report
      2010 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      Saino-Saito S, Nourani RM, Iwasa H, Kondo H, Owada Y
    • Journal Title

      Cell Tiss Res 338(2)

      Pages: 191-201

    • Related Report
      2010 Final Research Report
  • [Journal Article]2009

    • Author(s)
      Suzuki R, Nourani MR, Saino-Saito S, Kondo H, Owada Y
    • Journal Title

      Histochem Cell Biol 132(6)

      Pages: 577-584

    • Related Report
      2010 Final Research Report
  • [Journal Article]2009

    • Author(s)
      Motohashi K, 他4名, Kondo H
    • Journal Title

      Yakugaku Zasshi 129(2)

      Pages: 191-195

    • Related Report
      2010 Final Research Report
  • [Journal Article] Discrete localization of fatty acid binding proteins in various cell populations of mouse retina2009

    • Author(s)
      齋野幸子、近藤尚武, 他2名
    • Journal Title

      Cell Tissue Research 338

      Pages: 191-201

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization of fatty acid binding protein of epidermal type common to dendritic cells and presumptive macrophages in Peyer's patches and epithelial M cells of mouse intestine2009

    • Author(s)
      齋野幸子、近藤尚武, 他7名
    • Journal Title

      Histochem Cell Biol 132

      Pages: 577-584

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization of EFA6A, a guanine nucleotide exchange factor for ARF6, in spermatogenic cells of testes of adult mouse2009

    • Author(s)
      齋野幸子、近藤尚武, 他4名
    • Journal Title

      J Molecular Histology 40

      Pages: 77-80

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization of EFA6A, a guanine nucleotide exchange factor for ARF6, in spermatogenic cells of testes of adult mice.2009

    • Author(s)
      Suzuki R, Kondo H, 他4名.
    • Journal Title

      Journal of Molecular Histology (英国) 40

      Pages: 77-80

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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