DNA fragmentation of mouse small intestinal epithelial cells by iIEL and DNA repair in the epithelial cells
| Project/Area Number |
20590181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tohoku University |
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Principal Investigator |
OGATA Masaki Tohoku University, 大学院・医学系研究科, 助教 (50311907)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Tsunetoshi 東北大学, 大学院・医学系研究科, 教授 (90004746)
MATSUTANI Takaji 東北大学, 大学院・医学系研究科, 助教 (70372290)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | DNA断片化 / DNA修復 / 小腸 / iIEL / 上皮細胞 / 免疫組織化学 / 電子顕微鏡 / 抗CD3抗体 / in vivo |
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| Research Abstract |
In the in vivo mouse experiment system in which mice were intraperitoneally given anti-CD3 antibody(Ab), DNA fragmentation(DNA-Frag) was induced in intestinal epithelial cells (IEC), and we immunohistochemically observed the subsequent accumulation and recruitment of DNA-repair related proteins in the DNA-fragmented IEC, and further confirmed that once-fragmented-DNA is repaired rapidly within 60 min. DNA-Frag per se does not necessarily lead to cell death. IECs survived after DNA-Frag and repaired DNA. DNA-Frag by itself was found to be not an essential indication of cell death.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] The interaction of monocytes with rheumatoid synovial cells is a key step in LIGHT-mediated inflammatory bone destruction.2009
Author(s)
Ishida S, Yamane S, Nakano S, Yanagimoto T, Hanamoto Y, Maeda-Tanimura M, Toyosaki-Maeda T, Ishizaki J, Matsuo Y, Fukui N, Itoh T, Ochi T, Suzuki R.
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Journal Title
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[Journal Article] T cell receptor repertoire in BALB/c mice varies according to tissue type, sex, age, and hydrocortisone treatment.2009
Author(s)
Kitaura K, Kanayama K, Fujii Y, Shiobara N, Tanaka K, Kurane I, Suzuki S, Itoh T, Suzuki R.
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Journal Title
Experimental Animals. 58
Pages: 159-168
NAID
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[Journal Article] Absence of Ku70 gene obliterates X-ray-induced lacZ mutagenesis of small deletions in mouse tissues.2008
Author(s)
Uehara Y, Ikehata H, Komura J, Ito A, Ogata M, Itoh T, Hirayama R, Furusawa Y, Ando K, Paunesku T, Woloschak GE, Komatsu K, Matsuura S, Ikura T, Kamiya K, Ono T.
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Journal Title
Radiation Research. 170
Pages: 216-223
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Peer Reviewed
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[Journal Article] Proinflammatory role of amphiregulin, an epidermal growth factor family member whose expression is augmented in rheumatoid arthritis patients.2008
Author(s)
Yamane S, Ishida S, Hanamoto Y, Kumagai K, Masuda R, Tanaka K, Shiobara N, Yamane N, Mori T, Juji T, Fukui N, Itoh T, Ochi T, Suzuki R.
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Journal Title
Journal of Inflammation (Lond). 5
Pages: 5-5
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[Journal Article] Discovery of novel non-peptide thrombopoietin mimetic compounds that induce megakaryocytopoiesis.2008
Author(s)
Yamane N, Takahashi K, Tanaka Y, Kato K, Takayama M, Ohyabu N, Shiota T, Takenaka H, Yoshida Y, Hara S, Murashi T, Nakamura E, Nishitani Y, Ishizaki J, Yamane S, Nagata K, Koizumi K, Yutsudo T, Suzuki R, Itoh T, Takemoto H.
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Journal Title
Bioscience Reports. 28
Pages: 275-285
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[Journal Article] Inhibition of Src homology 2 domain-containing protein tyrosine phosphatase substrate-1 reduces the severity of collagen-induced arthritis.2008
Author(s)
Tanaka K, Horikawa T, Suzuki S, Kitaura K, Watanabe J, Gotoh A, Shiobara N, Itoh T, Yamane S, Suzuki R, Fukui N, Ochi T.
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Journal Title
The Journal of Rheumatology. 35
Pages: 2316-2324
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