| Project/Area Number |
20590181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tohoku University |
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Principal Investigator |
OGATA Masaki Tohoku University, 大学院・医学系研究科, 助教 (50311907)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Tsunetoshi 東北大学, 大学院・医学系研究科, 教授 (90004746)
MATSUTANI Takaji 東北大学, 大学院・医学系研究科, 助教 (70372290)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | DNA断片化 / DNA修復 / 小腸 / iIEL / 上皮細胞 / 免疫組織化学 / 電子顕微鏡 / 抗CD3抗体 / in vivo |
|---|
| Research Abstract |
In the in vivo mouse experiment system in which mice were intraperitoneally given anti-CD3 antibody(Ab), DNA fragmentation(DNA-Frag) was induced in intestinal epithelial cells (IEC), and we immunohistochemically observed the subsequent accumulation and recruitment of DNA-repair related proteins in the DNA-fragmented IEC, and further confirmed that once-fragmented-DNA is repaired rapidly within 60 min. DNA-Frag per se does not necessarily lead to cell death. IECs survived after DNA-Frag and repaired DNA. DNA-Frag by itself was found to be not an essential indication of cell death.
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