Analysis of collagen fibril formation and degeneration by atomic force microscopy in liquid condition
| Project/Area Number |
20590186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Niigata University |
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Principal Investigator |
HOSHI Osamu 新潟大学, 医歯学系, 准教授 (10303124)
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| Co-Investigator(Kenkyū-buntansha) |
USHIKI Tatsuo 新潟大学, 医歯学系, 教授 (40184999)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | コラーゲン線維 / 原子間力顕微鏡 |
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| Research Abstract |
In the present study, we investigated that the mechanism of formation and degeneration of collagen fibrils by atomic force microscopy(AFM) in liquid condition. The results of the study are as follows.
1) We succeeded in the observation of segment-long-spacing(SLS) crystallites, which is the bundle of type I collagen molecules, by atomic force microscopy in liquid.
2) A human osteosarcoma cell line named NOS-1 is known to produce a large amount of type I collagen fibrils on the culture dish. The developing collagen fibrils were observed by AFM in phosphate-buffered saline.
3) We succeeded in the observation of collagen fibrils produced by NOS-1with high-speed AFM.
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Report
(4 results)
Research Products
(5 results)
