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Analysis of collagen fibril formation and degeneration by atomic force microscopy in liquid condition

Research Project

Project/Area Number 20590186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNiigata University

Principal Investigator

HOSHI Osamu  新潟大学, 医歯学系, 准教授 (10303124)

Co-Investigator(Kenkyū-buntansha) USHIKI Tatsuo  新潟大学, 医歯学系, 教授 (40184999)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsコラーゲン線維 / 原子間力顕微鏡
Research Abstract

In the present study, we investigated that the mechanism of formation and degeneration of collagen fibrils by atomic force microscopy(AFM) in liquid condition. The results of the study are as follows.
1) We succeeded in the observation of segment-long-spacing(SLS) crystallites, which is the bundle of type I collagen molecules, by atomic force microscopy in liquid.
2) A human osteosarcoma cell line named NOS-1 is known to produce a large amount of type I collagen fibrils on the culture dish. The developing collagen fibrils were observed by AFM in phosphate-buffered saline.
3) We succeeded in the observation of collagen fibrils produced by NOS-1with high-speed AFM.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (5 results)

All 2010 2008

All Presentation (5 results)

  • [Presentation] 培養細胞系を用いたコラーゲン線維形成過程の原子間力顕微鏡による構造解析2010

    • Author(s)
      星治,牛木辰男
    • Organizer
      日本解剖学会第115回総会・全国学術集会
    • Place of Presentation
      岩手
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 培養細胞系を用いたコラーゲン線維形成過程の液中原子間力顕微鏡による解析2010

    • Author(s)
      星治、牛木辰男
    • Organizer
      第115回日本解剖学会総会・全国学術集会
    • Place of Presentation
      岩手県・盛岡市
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Collagen fibril formation by human osteosarcoma cellsobservd by atomic force microscopy2010

    • Author(s)
      Hoshi O and Ushiki T
    • Organizer
      The 12th International Scanning Probe Microscopy Conference
    • Place of Presentation
      Sapporo
    • Related Report
      2010 Final Research Report
  • [Presentation] コラーゲン成分の液中AFM観察2008

    • Author(s)
      尾崎明加,星治,繁野雅次,中島邦雄,牛木辰男
    • Organizer
      日本顕微鏡学会第64回学術講演会
    • Place of Presentation
      京都
    • Year and Date
      2008-05-21
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Collagen fibrils and segment-long-spacing crystallities studied by atomic force microscopy2008

    • Author(s)
      S Ozaki, M Shigeno, KNakajima, T Ushiki
    • Organizer
      International meeting on AFM in life sciecnce and medicine (AFM Biomed Conference)
    • Place of Presentation
      Monterey, USA
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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