| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
It has been known that inositolphospholipid PI(4,5)P_2, whose pool is mainly localized in the plasma membrane, has some cellular functions, such as signal transduction, endocytosis, cytoskeleton regulation and so on. In this study, we show that, using freeze-fracture replica labeling method, the time course of reduction in the PI(4,5)P_2 level in the plasma membrane is different in each area of the undifferential plasma membrane, caveola, coated pit when cells are stimulated with angiotensin II or Ca^<2+> ionophore. These results indicate that PI(4,5)P_2 is compartmentalized in the plasma membrane, resulting in changing the level of PI(4,5)P_2 in the limited area.
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