Analysis of lipid-based signal transduction
Project/Area Number |
20590189
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General anatomy (including Histology/Embryology)
|
Research Institution | Nagoya University |
Principal Investigator |
FUJITA Akikazu Nagoya University, 医学系研究科, 准教授 (60282232)
|
Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Toyoshi 名古屋大学, 大学院・医学系研究科, 教授 (50115929)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 電子顕微鏡 / 細胞内情報伝達 / カベオラ / 細胞内オルガネラ / 急速凍結 / フリーズフラクチャー / イノシトールリン脂質 / リン脂質 / チトクローム / アンギオテンシンII |
Research Abstract |
It has been known that inositolphospholipid PI(4,5)P_2, whose pool is mainly localized in the plasma membrane, has some cellular functions, such as signal transduction, endocytosis, cytoskeleton regulation and so on. In this study, we show that, using freeze-fracture replica labeling method, the time course of reduction in the PI(4,5)P_2 level in the plasma membrane is different in each area of the undifferential plasma membrane, caveola, coated pit when cells are stimulated with angiotensin II or Ca^<2+> ionophore. These results indicate that PI(4,5)P_2 is compartmentalized in the plasma membrane, resulting in changing the level of PI(4,5)P_2 in the limited area.
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Report
(4 results)
Research Products
(42 results)