Application of transposon based transgene technique for mammals

• Project/Area Number: 20590190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka University
• Principal Investigator: YAGITA Kazuhiro Osaka University, 医学研究科, 教授 (90324920)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: トランスポゾン / 概日リズム / ES細胞 / Tol2 / 生体リズム / iPS細胞 / 発生 / 遺伝子導入 / 分化 / 細胞時計 / 遺伝子導入法

Research Abstract

Circadian clock resides in most of cells in our body, and it is important to observe the circadian clock oscillation in various functional tissues. In this study, using Tol2 transposon based vector, we have succeeded to observe the development of circadian oscillator during the ES cell differentiation in vitro.

Research Products

• [Journal Article] A Simple and highly efficient transgenesis method in mice with the Tol2 transposon system and cytoplasmic microinjection. (2010)
  • Author(s): Sumiyama K, Kawakami K, Yagita K
  • Journal Title: Genomics 95 Pages: 306-311
  • Peer Reviewed
• [Journal Article] Development of circadian oscillator during differentiation of mouse embryonic stem cell in vitro. (2010)
  • Author(s): Yagita K, Horie K, Koinuma S, Nakamura W, Yamanaka I, Urasaki A, Shigeyoshi Y, Kawakami K, Shimada S, Takeda J, Uchiyama Y
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 3846-3851
  • Peer Reviewed
• [Journal Article] Real-time monitoring of circadian clock oscillations in primary culture of mammalian cells using Tol2 transposon-mediated gene transfer strategy. (2010)
  • Author(s): Yagita K, Yamanaka I, Emoto N, Kawakami K, Shimada S
  • Journal Title: BMC Biotechnology 10
  • Peer Reviewed
• [Journal Article] A Simple and highly efficient transgenesis method in mice with the Tol2 transposon system and cytoplasmic microinjection. (2010)
  • Author(s): Sumiyama K, Kawakami K, Yagita K.
  • Journal Title: Genomics Volume: 95 Pages: 306-311
  • Peer Reviewed
• [Journal Article] Development of circadian oscillator during differentiation of mouse embryonic stem cells in vitro (2010)
  • Author(s): Yagita, K., 他
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 107 Pages: 3846-3851
  • Peer Reviewed
• [Journal Article] Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy (2010)
  • Author(s): Yagita, K., 他
  • Journal Title: BMC Biotechnology 10
  • Peer Reviewed
• [Journal Article] Miniscreening of kinase inhibitors affecting period-length of mammalian cellular circadian clock. (2009)
  • Author(s): Yagita K, Yamanaka I, Koinuma S, Shigeyoshi Y, Uchiyama Y
  • Journal Title: Acta Histochem. Cytochem. 42 Pages: 89-93
  • NAID: 130000118206
  • Peer Reviewed
• [Journal Article] Mini screening of kinase inhibitors affecting period-length of mammalian cellular circadian clock (2009)
  • Author(s): Yagita, K., 他
  • Journal Title: Acta Histochem. Cytochem. 42 Pages: 89-93
  • NAID: 130000118206
  • Peer Reviewed
• [Journal Article] The resetting of the circadian rhythm by Prostagland inJ2 is distinctly phase dependent (2009)
  • Author(s): S. Koinuma, K. Yagita, 他4名
  • Journal Title: FEBS Letters 583 Pages: 413-418
  • Peer Reviewed
• [Journal Article] Detection of a circadian enhancer in the mDbp promoter using prokaryotic transposon vector based strategy. (2008)
  • Author(s): Kiyohara, Y, Nishii K, Ukai-Tadenuma M, Ueda HR, Uchiyama Y, Yagita K
  • Journal Title: Nucl.Acids Res. 36
  • Peer Reviewed
• [Presentation] Development of Circadian Oscillator in Mammals. (2010)
  • Author(s): 八木田和弘
  • Organizer: SRBR 2010 Meeting of the Society for Research on Biological Rhythms
  • Place of Presentation: Destin, USA
  • Year and Date: 2010-05-25
• [Presentation] Development of Circadian Oscillator in Mammals (2010)
  • Author(s): 八木田和弘
  • Organizer: SRBR 2010 Meeting of the Society for Research on Biological Rhythms
  • Place of Presentation: Destin, U.S.A(招待講演)
  • Year and Date: 2010-05-25
• [Presentation] ES細胞の分化誘導過程における概日時計の発生 (2009)
  • Author(s): 八木田和弘
  • Organizer: 第7回 北海道大学 脳科学研究教育センター・シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2009-12-08
• [Presentation] ES細胞の分化過程における概日時計振動体の形成 (2009)
  • Author(s): 八木田和弘
  • Organizer: 第15回 日本時間生物学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-27
• [Presentation] Development of circadian oscillator during the mouse ES cell differentiation in vitro (2009)
  • Author(s): 八木田和弘
  • Organizer: 第82回 日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] Chronogenesis during the ES cell differentiation in vitro (2009)
  • Author(s): 八木田和弘
  • Organizer: 第32回 日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Development of circadian oscillator during the mouse ES cell differentiation in vitro. (2009)
  • Author(s): 八木田和弘
  • Organizer: Gordon Research Conference : Chronobiology
  • Place of Presentation: Newport, USA
  • Year and Date: 2009-07-23
• [Presentation] Development of circadian oscillator during the mouse ES cell differentiation in vitro (2009)
  • Author(s): 八木田和弘
  • Organizer: Gordon Research Conference, Chronobiology
  • Place of Presentation: Newport, U.S.A.
  • Year and Date: 2009-07-23
• [Presentation] Chronogenesis during the ES cell differentiation in vitro. (2009)
  • Author(s): 八木田和弘
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Development of circadian oscillator during the mouse ES cell differentiation in vitro. (2009)
  • Author(s): 八木田和弘
  • Organizer: 第82回日本生化学会大
  • Place of Presentation: 神戸
• [Presentation] ES細胞と脳機能の発生 (2009)
  • Author(s): 八木田和弘
  • Organizer: 北海道大学脳科学研究教育センター・シンポジウム
  • Place of Presentation: 札幌
• [Presentation] Molecular network of mammalian circadian oscillator : period-1ength, phase and feedback-1oop. (2008)
  • Author(s): 八木田和弘
  • Organizer: 日本分子生物学会日本生化学会合同大会(BMB2 0 0 8)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-11