Paracellular permeability of tight junction examined by different combination of claudins or claudin-1 mutants.

• Project/Area Number: 20590193
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kyushu University
• Principal Investigator: INAI Tetsuichiro Kyushu University, 歯学部, 教授 (00264044)
• Co-Investigator(Kenkyū-buntansha): SHIBATA Yosaburo 九州大学, 医学研究院, 教授 (90037482) ; HIROSE Eiji 九州大学, 医学研究院, 助教 (40380620)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: タイト結合 / クローディン / 細胞間透過性 / フリーズフラクチャー / 共焦点顕微鏡 / tight junction / claudin / paracellular permeability / freeze fracture / confocal microscopy / extracellular loop / green fluorescent protein / transepithelial electrical resistance

Research Abstract

We found by comparative analysis of mouse rectum CMT93-I and -II cells that expression of claudin-2 caused increase of paracellular permeability. This suggests that tight junction function may depend on combination of claudin species expressed in the cells. We found that two cysteine residues (54C, 64C) conserved in the first extracellular loop of claudin-1 were necessary for the formation of tight junction strands and that replacement of 54C to alanine caused increase of transepithelial electrical resistance.

Research Products

• [Journal Article] Subcellular localization of tektin2 in rat sperm flagellum (2010)
  • Author(s): Shimasaki S, Yamamoto E, Murayama E, Kurio H, Kaneko T, Shibata Y, Inai T, Iida H
  • Journal Title: Zoolog Sci Vol.27, No.9 Pages: 755-61
  • NAID: 40017358639
  • Peer Reviewed
• [Journal Article] The protoplasmic or exoplasmic face association of tight junction particles cannot predict paracellular permeability or heterotypic claudin compatibility (2010)
  • Author(s): Inai T, Kamimura T, Hirose E, Iida H, Shibata Y
  • Journal Title: Eur J Cell Biol Vol.89, No.7 Pages: 547-556
  • Peer Reviewed
• [Journal Article] Characterization of NaV1.6-mediated Na+ currents in smooth muscle cells isolated from mouse vas deferens (2010)
  • Author(s): Zhu HL, Shibata A, Inai T, Nomura M, Shibata Y, Brock JA, Teramoto N
  • Journal Title: J Cell Physiol 223(1) Pages: 234-43
  • Peer Reviewed
• [Journal Article] 最近の研究と技術 タイト結合研究の新展開 (2010)
  • Author(s): 稲井哲一朗
  • Journal Title: 顕微鏡 45巻、3号 Pages: 190-192
• [Journal Article] タイト結合の形態と機能 (2010)
  • Author(s): 稲井哲一朗
  • Journal Title: 福岡歯大誌 36巻、3号 Pages: 101-110
  • NAID: 110007817661
  • Peer Reviewed
• [Journal Article] Subcellular localization of tektin2 in rat sperm flagellum (2010)
  • Author(s): Shimasaki S, Yamamoto E, Murayama E, Kurio H, Kaneko T, ShibataY, Inai T, Iida H
  • Journal Title: Zoolog Sci Volume: 27 Pages: 755-761
  • NAID: 40017358639
  • Peer Reviewed
• [Journal Article] The protoplasmic or exoplasmic face association of tight junction particles cannot predict paracellular permeability or heterotypic claudin compatibility (2010)
  • Author(s): Inai T, Kamimura T, Hirose E, Iida H, Shibata Y
  • Journal Title: Eur J Cell Biol Volume: 89 Pages: 547-556
  • Peer Reviewed
• [Journal Article] Characterization of NaV1.6-mediated Na+ currents in smooth muscle cells isolated from mouse vas deferens (2010)
  • Author(s): Zhu HL, Shibata A, Inai T, Nomura M, Shibata Y, Brock JA, Teramoto N
  • Journal Title: J Cell Physiol Volume: 223 Pages: 234-243
  • Peer Reviewed
• [Journal Article] 最近の研究と技術 タイト結合研究の新展開 (2010)
  • Journal Title: 顕微鏡 Volume: 45 Pages: 190-192
• [Journal Article] タイト結合の形態と機能 (2010)
  • Author(s): 稲井哲一朗
  • Journal Title: 福岡歯大誌 Volume: 36 Pages: 101-110
  • NAID: 110007817661
  • Peer Reviewed
• [Journal Article] The protoplasmic or exoplasmic face association of tight junction particles cannot predict paracellular permeability or heterotypic claudin compatibility (2010)
  • Author(s): T.Inai, et al.
  • Journal Title: Eur J Cell Biol (in press)
  • Peer Reviewed
• [Journal Article] Dynamic changes in amniotic tight junctions during pregnancy (2009)
  • Author(s): Kobayashi K, Inai T, Shibata Y, Yasui M
  • Journal Title: Placenta Vol.30, No.10 Pages: 840-7
  • Peer Reviewed
• [Journal Article] Freeze-fracture electron microscopic study of tight junction strands in HEK293 cells and MDCK II cells expressing claudin-1 mutants in the second extracellular loop (2009)
  • Author(s): Inai T, Sengoku A, Hirose E, Iida H, Shibata Y
  • Journal Title: Histochem Cell Biol Vol.131, No.6 Pages: 681-690
  • Peer Reviewed
• [Journal Article] Heterogeneous expression of endothelial connexin (Cx) 37, Cx40, and Cx43 in rat large veins (2009)
  • Author(s): 稲井哲一朗, 柴田洋三郎
  • Journal Title: Anat Sci Int Vol.84 Pages: 237-245
  • NAID: 10025550949
  • Peer Reviewed
• [Journal Article] Dynamic changes in amniotic tight junctions during pregnancy (2009)
  • Author(s): K.Kobayashi, et al.
  • Journal Title: Placenta 30 Pages: 840-847
  • Peer Reviewed
• [Journal Article] Freeze-fracture electron microscopic study of tight junction strands in HEK293 cells and MDCK II cells expressing claudin-1 mutants in the second extracellular loop (2009)
  • Author(s): T.Inai, et al.
  • Journal Title: Histochem Cell Biol 131 Pages: 681-690
  • Peer Reviewed
• [Journal Article] Intron retention generates a novel isoform of CEACAM6 that may act as an adhesion molecule in the ectoplasmic specialization structures between spermatids and Sertoli cells in rat testis (2008)
  • Author(s): Kurio H, Murayama E, Kaneko T, Shibata Y, Inai T, Iida H
  • Journal Title: Biol Reprod Vol.79, No.6 Pages: 1062-73
  • Peer Reviewed
• [Journal Article] Differential expression of the tight junction proteins, claudin-1, claudin-4, occludin, ZO-1, and PAR3, in the ameloblasts of rat upper incisors (2008)
  • Author(s): Inai T, Sengoku A, Hirose E, Iida H, Shibata Y
  • Journal Title: Anat Rec Vol.291, No.5 Pages: 577-85
  • Peer Reviewed
• [Journal Article] Tektin5, a new Tektin family member, is a component of the middle piece of flagella in rat spermatozoa (2008)
  • Author(s): Murayama E, Yamamoto E, Kaneko T, Shibata Y, Inai T, Iida H
  • Journal Title: Mol Reprod Dev Vol.75, No.4 Pages: 650-8
  • Peer Reviewed
• [Journal Article] Formation of aberrant TJ strands by overexpression of claudin-15 in MDCK II cells (2008)
  • Author(s): Sengoku A, Inai T, Shibata Y
  • Journal Title: Histochem Cell Biol Vol.129, No.2 Pages: 211-22
  • Peer Reviewed
• [Journal Article] Comparative characterization of mouse rectum CMT93-I and -II cells by expression of claudin isoforms and tight junction morphology and function (2008)
  • Author(s): Inai T, Sengoku A, Hirose E, Iida H, Shibata Y
  • Journal Title: Histochem Cell Biol Vol.129, No.2 Pages: 223-32
  • Peer Reviewed
• [Journal Article] タイト結合の構造と機能 (2008)
  • Author(s): 稲井哲一朗、柴田洋三郎
  • Journal Title: 福岡医学雑誌 99巻 Pages: 25-31
  • NAID: 120000979956
  • Peer Reviewed
• [Journal Article] Intron Retention Generates a Novel Isoform of CEACAM6 That May Act as an Adhesion Molecule in the Ectoplasmic Specialization structures Between Spermatids and Sertoli Cells in Rat Testis. (2008)
  • Author(s): H. Kurio, et al.
  • Journal Title: Biol. Reprod. 79 Pages: 1062-73
  • Peer Reviewed
• [Journal Article] Tektin5, a new Tektin family member, is a component of the middle piece of flagella in rat spermatozoa. (2008)
  • Author(s): E. Murayama, et al.
  • Journal Title: Mo1. Reprod. Dev. 75 Pages: 650-8
  • Peer Reviewed
• [Presentation] タイト結合膜蛋白claudin-10bとclaudin-15による形態と機能の解析 (2010)
  • Author(s): 稲井哲一朗
  • Organizer: 第52回日本顕微鏡学会九州支部総会・学術講演会
  • Place of Presentation: 九州大学病院地区
  • Year and Date: 2010-12-04
• [Presentation] タイト結合の形態と機能 (2010)
  • Author(s): 稲井哲一朗
  • Organizer: 日本解剖学会第66回九州支部学術集会
  • Place of Presentation: 福岡県歯科医師会館(特別講演)
  • Year and Date: 2010-10-09
• [Presentation] タイト結合の形態と機能 (2010)
  • Author(s): 稲井哲一朗
  • Organizer: 日本解剖学会第66回九州支部学術集会
  • Place of Presentation: 福岡県歯科医師会館
  • Year and Date: 2010-10-09
• [Presentation] タイト結合膜蛋白 claudin とタイト結合の形態と機能についての研究 (2010)
  • Author(s): 稲井哲一朗, ほか
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手医科大学
  • Year and Date: 2010-03-30
• [Presentation] タイト結合膜蛋白claudinとタイト結合の形態と機能についての研究 (2010)
  • Author(s): 稲井哲一朗, ほか
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手医科大学
• [Presentation] タイト結合ストランドの形態と細胞間透過性 (2009)
  • Author(s): 稲井哲一朗, ほか
  • Organizer: 日本解剖学会・第65回九州支部学術集会
  • Place of Presentation: 琉球大学医学部
  • Year and Date: 2009-11-07
• [Presentation] イントロンリテンションにより産出される新規接着分子Ceacam6-Lの精巣における発現と局在 (2009)
  • Author(s): 飯田弘ら
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2009-03-28
• [Presentation] タイト結合ストランドの形態は細胞間透過性と関連するか? (2009)
  • Author(s): 稲井哲一朗, ほか
  • Organizer: 第8回コネキシン研究会
  • Place of Presentation: 九州大学医学部
• [Presentation] タイト結合ストランドの形態とclaudin-1の細胞外第二ループとの関連 (2009)
  • Author(s): 稲井哲一朗, ほか
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山理科大学
• [Presentation] claudin-1の細胞外第二ループとタイト結合の形態 (2008)
  • Author(s): 稲井哲一朗ら
  • Organizer: 第7回コネキシン研究会
  • Place of Presentation: ホテルルビノ京都堀川
  • Year and Date: 2008-12-20
• [Presentation] claudin-1の細胞外第二ループの変異によるタイト結合の形態の変化 (2008)
  • Author(s): 稲井哲一朗, ほか
  • Organizer: 第50回日本顕微鏡学会九州支部総会
  • Place of Presentation: 久留米大学医学部
  • Year and Date: 2008-12-06
• [Presentation] claudin-1の細胞外第二ループの変異によるタイト結合の形態の変化 (2008)
  • Author(s): 稲井哲一朗ら
  • Organizer: 第50回日本顕微鏡学会九州支部総会・学術講演会
  • Place of Presentation: 久留米大学医学部
  • Year and Date: 2008-12-06
• [Presentation] Freeze-fracture electron microscopy of tight junction strands formed by expression of claudin-l mutants in the second extracellular loop (2008)
  • Author(s): T. Inai, et al.
  • Organizer: The 9th Asia-Pacific Microscopy Conference
  • Place of Presentation: Jeju International Convention Center, Jeju island, Korea
  • Year and Date: 2008-11-04
• [Presentation] claudin-1の細胞外第二ループの変異によるタイト結合の形態と機能の変化 (2008)
  • Author(s): 稲井哲一朗, ほか
  • Organizer: 日本解剖学会・第64回九州支部学術集会
  • Place of Presentation: 福岡大学医学部
  • Year and Date: 2008-10-25
• [Presentation] claudin-1の細胞外第二ループの変異によるタイト結合の形態と機能の変化 (2008)
  • Author(s): 稲井哲一朗ら
  • Organizer: 日本解剖学会第64回九州支部学術集会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-10-25
• [Presentation] claudin-1の細胞外第二ループとタイト結合の形態 (2008)
  • Author(s): 稲井哲一朗, ほか
  • Organizer: 第7回コネキシン研究会
  • Place of Presentation: 京都(京都府立医科大学主催)
• [Presentation] Freeze-fracture electron microscopy of tight junction strands formed by expression of claudin-1 mutants in the second extracellular loop. L06-32, poster (2008)
  • Author(s): 稲井哲一朗, et al
  • Organizer: 9th Asia-Pacific Microscopy Conference (APMC9)
  • Place of Presentation: ICC Jeju, Jeju island, Korea
• [Remarks] ホームページ等