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Paracellular permeability of tight junction examined by different combination of claudins or claudin-1 mutants.

Research Project

Project/Area Number 20590193
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyushu University

Principal Investigator

INAI Tetsuichiro  Kyushu University, 歯学部, 教授 (00264044)

Co-Investigator(Kenkyū-buntansha) SHIBATA Yosaburo  九州大学, 医学研究院, 教授 (90037482)
HIROSE Eiji  九州大学, 医学研究院, 助教 (40380620)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsタイト結合 / クローディン / 細胞間透過性 / フリーズフラクチャー / 共焦点顕微鏡 / tight junction / claudin / paracellular permeability / freeze fracture / confocal microscopy / extracellular loop / green fluorescent protein / transepithelial electrical resistance
Research Abstract

We found by comparative analysis of mouse rectum CMT93-I and -II cells that expression of claudin-2 caused increase of paracellular permeability. This suggests that tight junction function may depend on combination of claudin species expressed in the cells. We found that two cysteine residues (54C, 64C) conserved in the first extracellular loop of claudin-1 were necessary for the formation of tight junction strands and that replacement of 54C to alanine caused increase of transepithelial electrical resistance.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (42 results)

All 2010 2009 2008 Other

All Journal Article (24 results) (of which Peer Reviewed: 22 results) Presentation (17 results) Remarks (1 results)

  • [Journal Article] Subcellular localization of tektin2 in rat sperm flagellum2010

    • Author(s)
      Shimasaki S, Yamamoto E, Murayama E, Kurio H, Kaneko T, Shibata Y, Inai T, Iida H
    • Journal Title

      Zoolog Sci Vol.27, No.9

      Pages: 755-61

    • NAID

      40017358639

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The protoplasmic or exoplasmic face association of tight junction particles cannot predict paracellular permeability or heterotypic claudin compatibility2010

    • Author(s)
      Inai T, Kamimura T, Hirose E, Iida H, Shibata Y
    • Journal Title

      Eur J Cell Biol Vol.89, No.7

      Pages: 547-556

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of NaV1.6-mediated Na+ currents in smooth muscle cells isolated from mouse vas deferens2010

    • Author(s)
      Zhu HL, Shibata A, Inai T, Nomura M, Shibata Y, Brock JA, Teramoto N
    • Journal Title

      J Cell Physiol 223(1)

      Pages: 234-43

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 最近の研究と技術 タイト結合研究の新展開2010

    • Author(s)
      稲井哲一朗
    • Journal Title

      顕微鏡 45巻、3号

      Pages: 190-192

    • Related Report
      2010 Final Research Report
  • [Journal Article] タイト結合の形態と機能2010

    • Author(s)
      稲井哲一朗
    • Journal Title

      福岡歯大誌 36巻、3号

      Pages: 101-110

    • NAID

      110007817661

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subcellular localization of tektin2 in rat sperm flagellum2010

    • Author(s)
      Shimasaki S, Yamamoto E, Murayama E, Kurio H, Kaneko T, ShibataY, Inai T, Iida H
    • Journal Title

      Zoolog Sci

      Volume: 27 Pages: 755-761

    • NAID

      40017358639

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The protoplasmic or exoplasmic face association of tight junction particles cannot predict paracellular permeability or heterotypic claudin compatibility2010

    • Author(s)
      Inai T, Kamimura T, Hirose E, Iida H, Shibata Y
    • Journal Title

      Eur J Cell Biol

      Volume: 89 Pages: 547-556

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of NaV1.6-mediated Na+ currents in smooth muscle cells isolated from mouse vas deferens2010

    • Author(s)
      Zhu HL, Shibata A, Inai T, Nomura M, Shibata Y, Brock JA, Teramoto N
    • Journal Title

      J Cell Physiol

      Volume: 223 Pages: 234-243

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 最近の研究と技術 タイト結合研究の新展開2010

    • Journal Title

      顕微鏡

      Volume: 45 Pages: 190-192

    • Related Report
      2010 Annual Research Report
  • [Journal Article] タイト結合の形態と機能2010

    • Author(s)
      稲井哲一朗
    • Journal Title

      福岡歯大誌

      Volume: 36 Pages: 101-110

    • NAID

      110007817661

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The protoplasmic or exoplasmic face association of tight junction particles cannot predict paracellular permeability or heterotypic claudin compatibility2010

    • Author(s)
      T.Inai, et al.
    • Journal Title

      Eur J Cell Biol (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic changes in amniotic tight junctions during pregnancy2009

    • Author(s)
      Kobayashi K, Inai T, Shibata Y, Yasui M
    • Journal Title

      Placenta Vol.30, No.10

      Pages: 840-7

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Freeze-fracture electron microscopic study of tight junction strands in HEK293 cells and MDCK II cells expressing claudin-1 mutants in the second extracellular loop2009

    • Author(s)
      Inai T, Sengoku A, Hirose E, Iida H, Shibata Y
    • Journal Title

      Histochem Cell Biol Vol.131, No.6

      Pages: 681-690

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heterogeneous expression of endothelial connexin (Cx) 37, Cx40, and Cx43 in rat large veins2009

    • Author(s)
      稲井哲一朗, 柴田洋三郎
    • Journal Title

      Anat Sci Int Vol.84

      Pages: 237-245

    • NAID

      10025550949

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic changes in amniotic tight junctions during pregnancy2009

    • Author(s)
      K.Kobayashi, et al.
    • Journal Title

      Placenta 30

      Pages: 840-847

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Freeze-fracture electron microscopic study of tight junction strands in HEK293 cells and MDCK II cells expressing claudin-1 mutants in the second extracellular loop2009

    • Author(s)
      T.Inai, et al.
    • Journal Title

      Histochem Cell Biol 131

      Pages: 681-690

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intron retention generates a novel isoform of CEACAM6 that may act as an adhesion molecule in the ectoplasmic specialization structures between spermatids and Sertoli cells in rat testis2008

    • Author(s)
      Kurio H, Murayama E, Kaneko T, Shibata Y, Inai T, Iida H
    • Journal Title

      Biol Reprod Vol.79, No.6

      Pages: 1062-73

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential expression of the tight junction proteins, claudin-1, claudin-4, occludin, ZO-1, and PAR3, in the ameloblasts of rat upper incisors2008

    • Author(s)
      Inai T, Sengoku A, Hirose E, Iida H, Shibata Y
    • Journal Title

      Anat Rec Vol.291, No.5

      Pages: 577-85

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tektin5, a new Tektin family member, is a component of the middle piece of flagella in rat spermatozoa2008

    • Author(s)
      Murayama E, Yamamoto E, Kaneko T, Shibata Y, Inai T, Iida H
    • Journal Title

      Mol Reprod Dev Vol.75, No.4

      Pages: 650-8

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formation of aberrant TJ strands by overexpression of claudin-15 in MDCK II cells2008

    • Author(s)
      Sengoku A, Inai T, Shibata Y
    • Journal Title

      Histochem Cell Biol Vol.129, No.2

      Pages: 211-22

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative characterization of mouse rectum CMT93-I and -II cells by expression of claudin isoforms and tight junction morphology and function2008

    • Author(s)
      Inai T, Sengoku A, Hirose E, Iida H, Shibata Y
    • Journal Title

      Histochem Cell Biol Vol.129, No.2

      Pages: 223-32

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] タイト結合の構造と機能2008

    • Author(s)
      稲井哲一朗、柴田洋三郎
    • Journal Title

      福岡医学雑誌 99巻

      Pages: 25-31

    • NAID

      120000979956

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intron Retention Generates a Novel Isoform of CEACAM6 That May Act as an Adhesion Molecule in the Ectoplasmic Specialization structures Between Spermatids and Sertoli Cells in Rat Testis.2008

    • Author(s)
      H. Kurio, et al.
    • Journal Title

      Biol. Reprod. 79

      Pages: 1062-73

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tektin5, a new Tektin family member, is a component of the middle piece of flagella in rat spermatozoa.2008

    • Author(s)
      E. Murayama, et al.
    • Journal Title

      Mo1. Reprod. Dev. 75

      Pages: 650-8

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] タイト結合膜蛋白claudin-10bとclaudin-15による形態と機能の解析2010

    • Author(s)
      稲井哲一朗
    • Organizer
      第52回日本顕微鏡学会九州支部総会・学術講演会
    • Place of Presentation
      九州大学病院地区
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] タイト結合の形態と機能2010

    • Author(s)
      稲井哲一朗
    • Organizer
      日本解剖学会第66回九州支部学術集会
    • Place of Presentation
      福岡県歯科医師会館(特別講演)
    • Year and Date
      2010-10-09
    • Related Report
      2010 Final Research Report
  • [Presentation] タイト結合の形態と機能2010

    • Author(s)
      稲井哲一朗
    • Organizer
      日本解剖学会第66回九州支部学術集会
    • Place of Presentation
      福岡県歯科医師会館
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] タイト結合膜蛋白 claudin とタイト結合の形態と機能についての研究2010

    • Author(s)
      稲井哲一朗, ほか
    • Organizer
      第115回日本解剖学会総会・全国学術集会
    • Place of Presentation
      岩手医科大学
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] タイト結合膜蛋白claudinとタイト結合の形態と機能についての研究2010

    • Author(s)
      稲井哲一朗, ほか
    • Organizer
      第115回日本解剖学会総会・全国学術集会
    • Place of Presentation
      岩手医科大学
    • Related Report
      2010 Final Research Report
  • [Presentation] タイト結合ストランドの形態と細胞間透過性2009

    • Author(s)
      稲井哲一朗, ほか
    • Organizer
      日本解剖学会・第65回九州支部学術集会
    • Place of Presentation
      琉球大学医学部
    • Year and Date
      2009-11-07
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] イントロンリテンションにより産出される新規接着分子Ceacam6-Lの精巣における発現と局在2009

    • Author(s)
      飯田弘ら
    • Organizer
      第114回日本解剖学会総会・全国学術集会
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] タイト結合ストランドの形態は細胞間透過性と関連するか?2009

    • Author(s)
      稲井哲一朗, ほか
    • Organizer
      第8回コネキシン研究会
    • Place of Presentation
      九州大学医学部
    • Related Report
      2010 Final Research Report
  • [Presentation] タイト結合ストランドの形態とclaudin-1の細胞外第二ループとの関連2009

    • Author(s)
      稲井哲一朗, ほか
    • Organizer
      第114回日本解剖学会総会・全国学術集会
    • Place of Presentation
      岡山理科大学
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] claudin-1の細胞外第二ループとタイト結合の形態2008

    • Author(s)
      稲井哲一朗ら
    • Organizer
      第7回コネキシン研究会
    • Place of Presentation
      ホテルルビノ京都堀川
    • Year and Date
      2008-12-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] claudin-1の細胞外第二ループの変異によるタイト結合の形態の変化2008

    • Author(s)
      稲井哲一朗, ほか
    • Organizer
      第50回日本顕微鏡学会九州支部総会
    • Place of Presentation
      久留米大学医学部
    • Year and Date
      2008-12-06
    • Related Report
      2010 Final Research Report
  • [Presentation] claudin-1の細胞外第二ループの変異によるタイト結合の形態の変化2008

    • Author(s)
      稲井哲一朗ら
    • Organizer
      第50回日本顕微鏡学会九州支部総会・学術講演会
    • Place of Presentation
      久留米大学医学部
    • Year and Date
      2008-12-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Freeze-fracture electron microscopy of tight junction strands formed by expression of claudin-l mutants in the second extracellular loop2008

    • Author(s)
      T. Inai, et al.
    • Organizer
      The 9th Asia-Pacific Microscopy Conference
    • Place of Presentation
      Jeju International Convention Center, Jeju island, Korea
    • Year and Date
      2008-11-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] claudin-1の細胞外第二ループの変異によるタイト結合の形態と機能の変化2008

    • Author(s)
      稲井哲一朗, ほか
    • Organizer
      日本解剖学会・第64回九州支部学術集会
    • Place of Presentation
      福岡大学医学部
    • Year and Date
      2008-10-25
    • Related Report
      2010 Final Research Report
  • [Presentation] claudin-1の細胞外第二ループの変異によるタイト結合の形態と機能の変化2008

    • Author(s)
      稲井哲一朗ら
    • Organizer
      日本解剖学会第64回九州支部学術集会
    • Place of Presentation
      福岡大学
    • Year and Date
      2008-10-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] claudin-1の細胞外第二ループとタイト結合の形態2008

    • Author(s)
      稲井哲一朗, ほか
    • Organizer
      第7回コネキシン研究会
    • Place of Presentation
      京都(京都府立医科大学主催)
    • Related Report
      2010 Final Research Report
  • [Presentation] Freeze-fracture electron microscopy of tight junction strands formed by expression of claudin-1 mutants in the second extracellular loop. L06-32, poster2008

    • Author(s)
      稲井哲一朗, et al
    • Organizer
      9th Asia-Pacific Microscopy Conference (APMC9)
    • Place of Presentation
      ICC Jeju, Jeju island, Korea
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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