Paracellular permeability of tight junction examined by different combination of claudins or claudin-1 mutants.
| Project/Area Number |
20590193
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyushu University |
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Principal Investigator |
INAI Tetsuichiro Kyushu University, 歯学部, 教授 (00264044)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Yosaburo 九州大学, 医学研究院, 教授 (90037482)
HIROSE Eiji 九州大学, 医学研究院, 助教 (40380620)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | タイト結合 / クローディン / 細胞間透過性 / フリーズフラクチャー / 共焦点顕微鏡 / tight junction / claudin / paracellular permeability / freeze fracture / confocal microscopy / extracellular loop / green fluorescent protein / transepithelial electrical resistance |
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| Research Abstract |
We found by comparative analysis of mouse rectum CMT93-I and -II cells that expression of claudin-2 caused increase of paracellular permeability. This suggests that tight junction function may depend on combination of claudin species expressed in the cells. We found that two cysteine residues (54C, 64C) conserved in the first extracellular loop of claudin-1 were necessary for the formation of tight junction strands and that replacement of 54C to alanine caused increase of transepithelial electrical resistance.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Differential expression of the tight junction proteins, claudin-1, claudin-4, occludin, ZO-1, and PAR3, in the ameloblasts of rat upper incisors2008
Author(s)
Inai T, Sengoku A, Hirose E, Iida H, Shibata Y
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Journal Title
Anat Rec Vol.291, No.5
Pages: 577-85
Related Report
Peer Reviewed
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