Analysis of the changes in the molecular structure of basement membrane accompanying by the diabetic keratopathy
| Project/Area Number |
20590198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyorin University |
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Principal Investigator |
AKIMOTO Yoshihiro Kyorin University, 医学部, 准教授 (60184115)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖尿病 / 角膜症 / 基底膜 / 糖鎖 / 細胞外マトリックス / 糖修飾 / O-GlcNAc / プロテオミクス / 細胞骨格 / GK ラット / Wistar ラット / コラーゲン / ラミニン / フィプロネクチン / ナテグリニド / グリベンクラミド / フィブロネクチン / ヘパラン硫酸プロテオグリカン / Sendaiラット |
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| Research Abstract |
We examined changes in the ultrastructure of basement membrane of the cornea of diabetic GK rats. In the cornea of diabetic rats, many long-spacing collagen fibrils were observed in basement membrane. The antidiabetic agents significantly suppressed the formation of the long-spacing collagen. To explore biomarker proteins of which glcosylation level change in diabetic cornea, we carried out the proteomic analysis. Selected proteins that changed markedly in the O-GlcNAc level were identified by Mass Spectrometry analysis.
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] Proteomic analysis of two types of exosomes in human whole saliva.2011
Author(s)
Ogawa Y, Miura Y, Harazono A, Kanai-Azuma M, Akimoto Y, Kawakami H, Yamaguchi T, Toda T, Endo T, Tsubuki M, Yanoshita R
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Journal Title
Biol Pharm Bull 34
Pages: 13-23
NAID
Related Report
Peer Reviewed
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[Journal Article] Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis.2010
Author(s)
Ohara-Imaizumi M, Yoshida M, Aoyagi K, Saito T, Okamura T, Takenaka H, Akimoto Y, Nakamichi Y, Takanashi-Yanobu R, Nishiwaki C, Kawakami H, Kato N, Hisanaga S, Kakei M, Nagamatsu S
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Distribution and function of macrophage galactose-type C-type lectin 2 (MGL2/CD301b) : Efficient uptake and presentation of glycosylated antigens by dendritic cells.2010
Author(s)
Denda-Nagai K, Aida S, Saba K, Suzuki K, Moriyama S, Oo-puthinan S, Tsuiji M, Morikawa A, Kumamoto Y, Sugiura D, Kudo A, Akimoto Y, Kawakami H, Bovin NV, Irimura T
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Journal Title
J Biol Chem 285
Pages: 19193-19204
Related Report
Peer Reviewed
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[Journal Article] Amino acid transporter LAT3 is required for podocyte development and function.2009
Author(s)
Sekine Y, Nishibori Y, Akimoto Y, Kudo A, Ito N, Fukuhara D, Kurayama R, Higashihara E, Babu E, Kanai Y, Asanuma K, Nagata M, Majumdar A, Tryggvason K, Yan K
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Journal Title
J Am Soc Nephrol 20
Pages: 1586-1596
Related Report
Peer Reviewed
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[Journal Article] Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephritic syndrome.2009
Author(s)
Shimizu M, Khoshnoodi J, Akimoto Y, Kawakami H, Hirano H, Higashihara E, Hosoyamada M, Sekine Y, Kurayama R, Kurayama H, Joh K, Hirabayashi J, Kasai K, Tryggvason K, Ito N, Yan K
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Journal Title
Lab Invest 89
Pages: 178-195
Related Report
Peer Reviewed
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