| Project/Area Number |
20590200
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nippon Medical School |
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Principal Investigator |
ISHIBASHI Osamu Nippon Medical School, 医学部, 講師 (70293214)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIZAWA Toshihiro 日本医科大学, 大学院・医学研究科, 教授 (90271220)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 機能解剖学 / マイクロRNA / バイオイメージング / エクソソーム / 蛍光蛋白質 / Processing body / アデノウィルスベクター / Pocessing body / exosome / GW182 / CD-63 |
|---|
| Research Abstract |
MicroRNAs (miRNAs) are small RNAs that play a role in post-transcriptional regulation of gene expression. miRNAs are also secreted extracellularly via exosomes and can be detected in body fluids including blood; thus they could be promising biomarkers for various diseases. However, the intracellular dynamics, including a pathway prior to extracellular release, of miRNAs is not well understood. In this study, we performed bio-imaging analyses to examine intracellular dynamics of miRNAs and miRNA-associated proteins. Further, we performed the profiling analysis of miRNAs that are extracellularly secreted via exosomes to characterize them.
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