| Project/Area Number |
20590202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kinki University |
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Principal Investigator |
NAGANO Mamoru Kinki University, 医学部, 講師 (80155960)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGEYOSHI Yasufumi 近畿大学, 医学部, 教授 (20275192)
FUZIOKA Atuko 近畿大学, 医学部, 准教授 (30077664)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 視交叉上核 / 概日時計 / ペプチド受容体 / 腹外側部(VLSCN) / 背内側部(DMSCN) / VIP受容体(VPAC2) / AVP受容体(V1a) / GRP受容体(GRPR) / in situ hybridization / 光同調 / 体内時計 / VIP / Per1 / Per2 / プロテオーム解析 / 日長時間 / 概日リズム |
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| Research Abstract |
We investigated the photo signal transduction pathway in the suprachiasmatic nucleus (SCN), the mammalian circadian centers. By using in situ hybridization technique, we examined the localization of vasoactive intestinal peptide (VIP) mRNA and VPAC2 mRNA (one of the VIP receptors)-expressing neurons in the rat SCN. We found that VIP mRNA was strongly expressed in the ventrolateral SCN (VLSCN). In contrast, VPAC2 mRNA was strongly expressed in the dorsomedial SCN (DMSCN). Moreover, to investigate regional response to VIP, VIP administration to the SCN slice culture after isolation of DM from VL by a knife showed the large phase shift in only separating-DM. It is highly probable that VLSCN regulate the phase of DMSCN by VIP-VPAC2R. The findings suggest that VIP acting via the VPAC2 receptor is implicated as a key signaling pathway from VLSCN to DMSCN.
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