Molecular Cell Biological Analysis of Nuclear Condensation in Apoptosis

• Project/Area Number: 20590203
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kawasaki Medical School
• Principal Investigator: TONE Shigenobu Kawasaki Medical School, 医学部, 准教授 (70211399)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Takeaki 川崎医科大学, 医学部, 助教 (20398431)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 分子形態学 / アポトーシス / 核 / 凝縮

Research Abstract

This study characterized the condensation factor(s)which induced the ring condensation in nuclei during apoptosis using cell-free apoptosis system. Neither DNase, caspases nor JNK kinase would be involved in ring condensation.

Research Products

• [Journal Article] Deficient tryptophan catabolism along the kynurenine pathway reveals that the epididymis is in a unique tolerogenic state. (2011)
  • Author(s): Jrad-Lamine A, et al
  • Journal Title: Journal of Biological Chemistry 286 Pages: 8030-8042
  • Peer Reviewed
• [Journal Article] Hepatitis C virus and disrupted interferon signaling promote lymphoproliferation via type II CD95 and interleukins (2009)
  • Author(s): Machida K, et al
  • Journal Title: Gastroenterology 137 Pages: 285-96
  • Peer Reviewed
• [Journal Article] Imaging of mitotic cell division and apoptotic intra-nuclear processes in multicolor. (2009)
  • Author(s): Sugimoto K, Tone S
  • Journal Title: Methods in Molecular Biology 591 Pages: 135-146
• [Journal Article] Possible involvement of caspase-6 and -7 but not caspase-3 in the regulation of hypoxia-induced apoptosis in tube-forming endothelial cells. (2009)
  • Author(s): Eguchi R, Tone S, et al
  • Journal Title: Exp.Cell Res 315 Pages: 327-35
  • Peer Reviewed
• [Journal Article] Possible involvement of caspase-6 and -7 but not caspase-3 in the regulation of hypoxia-induced apoptosis in tube-forming endothelial cells (2009)
  • Author(s): Eguchi R
  • Journal Title: Exp.Cell Res. 315 Pages: 327-335
  • Peer Reviewed
• [Journal Article] Hepatitis C virus and disrupted interferon signaling promote lymphoproliferation via type II CD95 and interleukins (2009)
  • Author(s): Machida K
  • Journal Title: Gastroenterology 137 Pages: 285-296
  • Peer Reviewed
• [Journal Article] Expression of Immuno-Regulatory Molecules by Thyrocytes Protects NOD-H2h4 Mice from Developing Autoimmune Thyroiditis (2009)
  • Author(s): Nakahara M
  • Journal Title: Endocrinology 150 Pages: 1545-1551
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Sugimoto K
  • Journal Title: Methods in Molecular Biology : Live Cell Imaging : Imaging of mitotic cell division and apoptotic intra-nuclear processes in multicolor(Humana Press) Pages: 135-146
• [Journal Article] Possible involvement of caspase-6 and -7 but not caspase-3 in the regulation of hypoxia-induced apoptosis in tube-forming endothelial cells. (2009)
  • Author(s): Eguchi R
  • Journal Title: Exp. Cell Res. 315 Pages: 327-35
  • Peer Reviewed
• [Journal Article] Expression of Immuno-Regulatory Molecules by Thyrocytes Protects NOD-H2h4 Mice from Developing Autoimmune Thyroiditis. (2009)
  • Author(s): Nakahara M
  • Journal Title: Endocrinology 150 Pages: 1545-1551
  • Peer Reviewed
• [Journal Article] Hepatitis C virus and disrupted interferon signaling promote lytnphoproliferation via type II CD95 and interleukins. . (2009)
  • Author(s): Machida K
  • Journal Title: Gastroenterology (in press)
  • Peer Reviewed
• [Presentation] Proapoptotic Role of DNA-PK System in DT40 Apoptosis Induced by X-ray, UV or Hydrogen Peroxide (2010)
  • Author(s): Tone S, Kuribayashi F, Takata M, Ishiai M.
  • Organizer: American Society of Cell Biology
  • Place of Presentation: フィラデルフィア(米国)
  • Year and Date: 2010-12-13
• [Presentation] 特別講演 プログラムされた細胞死 アポトーシス (2010)
  • Author(s): 刀祢重信
  • Organizer: 大阪府立大学ライブセルイメージング研究所設立4周年記念シンポジウム
  • Place of Presentation: 大阪府堺市
  • Year and Date: 2010-02-01
• [Presentation] Proapoptotic Role of DNA-PK System in DT40 Apoptosis Induced by X-ray (2010)
  • Author(s): Tone S, Kuribayashi F, Takata M, Ishiai M
  • Organizer: UV or Hydrogen Peroxide American Society for Cell Biology Annual Meeting
  • Place of Presentation: Philadelphia, USA
• [Presentation] 細胞死関連マルチパラメーターの自動連続測定法-Image Xpress を用いて (2009)
  • Author(s): 刀祢重信
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-12
• [Presentation] ワークショップ アポトーシス検出法 ―初歩から最先端まで 1)アポトーシスの検出法 まずこれをやってみたら? (2009)
  • Author(s): 刀祢重信
  • Organizer: 第18回日本アポトーシス研究会学術集会
  • Place of Presentation: 長崎市
  • Year and Date: 2009-08-01
• [Presentation] 細胞を救うはずのDNA修復酵素DNA-PKは、細胞自らが死ぬために必要である (2009)
  • Author(s): 刀祢重信
  • Organizer: 第8回核ダイナミクス研究会
  • Place of Presentation: 静岡県伊豆市
  • Year and Date: 2009-06-19
• [Presentation] Ku70 and DNA-PKcs are essential in DT40 apoptosis induced by X-ray (2009)
  • Author(s): Tone S, Tanda K Takata M Ishiai M
  • Organizer: UV or H2O2 Keystone Symposia Conference "Cell Death Pathways"
  • Place of Presentation: Whistler, Canada
• [Presentation] Ku70 and DNA-PKcs are essential in DT40 apoptosis inducedby X-ray, UV or H2O2. (2009)
  • Author(s): Tone S
  • Organizer: Keystone Symposium on "Cell Death Pathways"
  • Place of Presentation: Wistler (Canada)
• [Presentation] Dynamic aspects of nuclear condensation revealed by time-lapse imaging, biochemical and electron microscopy analysis of cell-free apoptosis. (2007)
  • Author(s): Tone S, Tanda K Sugimoto K Suda T Uehira K, Kanouchi H Samejima K Minatogawa Y, Earnshaw W C
  • Organizer: EMBO Conference Series on Nuclear Structure & Dynamics.
  • Place of Presentation: Montpelier, France
• [Book] Methods in Molecular Biology : imaging of mitotic cell division and apoptotic intra-nuclear processes in multicolor (2009)
  • Author(s): Sugimoto K, Tone S. (in press)
  • Publisher: Humana Press
• [Remarks] 中高校生といのちの不思議を考える 細胞の自殺 アポトーシスの重要性
  • URL: http://www.terumozaidan.or.jp/labo/class/07/interview01.html