Physiological significance of synaptotagmin-SNAP25 binding in neurotransmitter release

• Project/Area Number: 20590209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Okayama University
• Principal Investigator: NISHIKI Teiichi Okayama University, 大学院・医歯薬学総合研究科, 助教 (70423340)
• Research Collaborator: MASUMOTO Toshio 岡山大学, 大学院・医歯薬学総合研究科, 大学院生 ; SUZUKI Koichiro 岡山大学, 大学院・医歯薬学総合研究科, 大学院生
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経科学 / シナプス伝達 / シナプス小胞 / カルシウムイオン / 開口放出

Research Abstract

To understand mechanisms of neurotransmitter release, the binding of synaptotagmin (syt) to SNARE complexes (SNAP-25-synatxin-synaptobrevin) was studied. Surprisingly, whereas SNAP-25 did not bind syt, which differs from previous findings, syntaxin bound syt in the absence of calcium ion (Ca^<2+>). Point mutagenesis and liposome fusion assay provide biochemical evidence that syt binds SNARE complexes via syntaxin before Ca2+ influx into presynaptic nerve terminals.

Research Products

• [Journal Article] SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming. (2010)
  • Author(s): Burgalossi A, Jung S, Meyer G, Jockusch WJ, Jahn O, Taschenberger H, O'Connor VM, Nishiki T, Takahashi M, Brose N, Rhee JS.
  • Journal Title: Neuron 68 Pages: 473-487
  • Peer Reviewed
• [Journal Article] Ca^<2+>-dependent dissociation of syntaxin 1 from synaptotagmin 1 (2010)
  • Author(s): Masumoto T, Nishiki T, Ohmori I, Tomizawa K, Matsui H
  • Journal Title: J Physiol Sci 60
• [Journal Article] Assembly and disassembly of the complex of synaptotagmin 1 and syntaxin 1 under physiological conditions (2010)
  • Author(s): Masumoto T, Nishiki T, Ohmori I, Matsui H
  • Journal Title: Neurosci Res 68 Pages: 228-228
• [Journal Article] SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming. (2010)
  • Author(s): Burgalossi A, Jung S, Meyer G, Jockusch WJ, Jahn O, Taschenberger H, O'Connor VM, Nishiki T, Takahashi M, Brose N, Rhee JS.
  • Journal Title: Neuron Volume: 68 Pages: 473-487
  • Peer Reviewed
• [Journal Article] Ca^<2+>-dependent dissociation of syntaxin 1 from synaptotagmin 1 (2010)
  • Author(s): Masumoto T, Nishiki T, Ohmori I, Tomizawa K, Matsui H
  • Journal Title: JOURNAL OF PHYSIOLOGICAL SCIENCES Volume: 60
• [Journal Article] Assembly and disassembly of the complex of synaptotagmin 1 and syntaxin 1 under physiological conditions (2010)
  • Author(s): Masumoto T, Nishiki T, Ohmori I, Matsui H
  • Journal Title: Neuroscience Research Volume: 68 Pages: 228-228
• [Journal Article] Negatively charged amino acid residues of syntaxin 1 critical for synaptotagmin 1 binding (2009)
  • Author(s): Masumoto T, Nishiki T, Omori I, Tomizawa K, Matsui H
  • Journal Title: Neurosci Res 65
• [Journal Article] Ca^<2+>-Independent SNARE Binding of Synaptotagmin I through Interaction between its C_2B Domain and Syntaxin 1 (2009)
  • Author(s): Masumoto T, Nishiki T, Omori I, Tomizawa K, Matsui H
  • Journal Title: J Physiol Sci 59 Pages: 141-141
• [Presentation] 大腸菌で発現させた組換えSNAREの天然のタンパク質とは異なるシナプトタグミンへの結合 (2011)
  • Author(s): 増本年男, ら
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 東日本大震災の発生により誌上開催
• [Presentation] シナプトタグミン1とシンタキシン1の生理的条件下における会合と解離 (2010)
  • Author(s): 増本年男, ら
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(神戸市)
  • Year and Date: 2010-09-03
• [Presentation] シナプトタグミン1はCa^<2+>依存的にシンタキシン1から解離する (2010)
  • Author(s): 増本年男, ら
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(盛岡市)
  • Year and Date: 2010-05-19
• [Presentation] 神経伝達物質放出に関わるシナプトタグミン1とSNARE複合体の結合部位の同定 (2009)
  • Author(s): 西木禎一, ら
  • Organizer: 第61回日本生理学会中国四国地方会
  • Place of Presentation: 山口大学医学部霜仁会館(宇部市)
  • Year and Date: 2009-11-22
• [Presentation] 神経伝達物質放出に関わるシナプトタグミン1とSNARE複合体の結合部位の同定 (2009)
  • Author(s): 西木禎一、増本年男、大守伊織、富澤一仁、松井秀樹
  • Organizer: 第61回 日本生理学会中国四国地方会
  • Place of Presentation: 山口大学医学部霜仁会館(宇部市)
  • Year and Date: 2009-11-22
• [Presentation] シンタキシンの負電荷を持つアミノ酸を介したシナプトタグミン1とのCa^<2+>非依存的結合 (2009)
  • Author(s): 増本年男, ら
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2009-09-16
• [Presentation] Negatively charged amino acid residues of syntaxin 1 critical for synaptotgmin 1 binding (2009)
  • Author(s): 西木禎一、増本年男、大守伊織、富澤一仁、松井秀樹
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2009-09-16
• [Presentation] Ca^<2+>-Independent SNARE Binding of Synaptotagmin I through Interaction between its C_2B Domain and Syntaxin 1. (2009)
  • Author(s): 増本年男, et al.
  • Organizer: 36^<th> Congress of the International Union of Physiological Sciences (IUPS2009)
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2009-07-28
• [Presentation] Ca^<2+>-INDEPENDENT SNARE BINDING OF SYNAPTOTAGMIN I THROUGH INTERACTION BETWEEN ITS C_2B DOMAIN AND SYNTAXIN 1 (2009)
  • Author(s): Masumoto T, Nishiki T, Omori I, Tomizawa K, Matsui H
  • Organizer: 36^<th> Congress of the International Union of Physiological Sciences(IUPS2009)
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2009-07-28
• [Remarks] ホームページ等
  • URL: http://seiri1.med.okayama-u.ac.jp