Temperature-sensitive recruitment of voltage-gated proton channels
Project/Area Number |
20590213
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General physiology
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Research Institution | Osaka City University |
Principal Investigator |
KUNO Miyuki Osaka City University, 大学院・医学研究科, 准教授 (00145773)
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Co-Investigator(Kenkyū-buntansha) |
SAKAI Hiromu 大阪市立大学, 大学院・医学研究科, 研究員 (90382192)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | プロトンチャネル / 温度感受性 / リクルートメント / マイクログリア / シグナル伝達 / 生理学 |
Research Abstract |
Voltage-gated proton channels (H^+ channels) are highly sensitive to temperature. The temperature-sensitivity of whole-cell H^+ currents consisted of two phases, a high Q_<10> phase over the lower temperature ranges (<~30℃) and a low Q_<10> phase at higher temperatures. We revealed the low Q_<10> phase represented the process of H^+ permeation through the channel pore and the access resistance to the pore, and the high Q_<10> phase, recruitment of active channels in addition to the permeation process. Actin cytoskeletal network and lipid raft structure are suggested to underlie the temperature-sensitive recruitment of H^+ channels.
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Report
(4 results)
Research Products
(75 results)
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[Journal Article] Temperature dependence of proton permeation through a voltage-gated proton channel.2009
Author(s)
Kuno M., Ando H., Morihata, H., Sakai H., Mori, H., Sawada, M., Oiki, S.
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Journal Title
J. Gen. Physiol. 134(3)
Pages: 191-205
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Peer Reviewed
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[Presentation] プロトンチャネル2010
Author(s)
久野みゆき
Organizer
同志社大学特別講義
Place of Presentation
同志社大学生命医科学部(京田辺)(招待講演)
Year and Date
2010-11-25
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[Presentation] Effects of general anesthetics on P2X4 receptors in microglia2010
Author(s)
Hasaka, M., Mori, T., Matsuura, T., Nishikawa, K., Kuno M., Asada, A.
Organizer
IARS 2010 Annual Meeting
Place of Presentation
Honolulu, Hawaii
Year and Date
2010-03-20
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[Presentation] Temperature dependence of proton permeation through a volage-gated proton channel in microglia.2010
Author(s)
Kuno M., Ando, H., Morihata, H., Sakai H., Mori, H., Oiki, S.
Organizer
54th Annual Meeting of American Biophysical Soceity
Place of Presentation
San Francisco, California
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[Presentation] Pacemaker channel HCN1 affects osteoclast function in vitro and bone remodeling in vivo.2009
Author(s)
Notomi T., Tanaka, S., Amano, H., Nakamura T., Noda, M., Skerry, T.M., Kuno M.
Organizer
31^<th> ASBMR Annual Meeting
Place of Presentation
Denver, Colorado (Plenary Poster Award)
Related Report
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[Presentation] Temperature-dependence of proton permeation through a voltage-gated proton channel in microglia.2009
Author(s)
Kuno M., Ando, H., Morihata, H., Sakai H., Mori, H., Shimizu, H., Iwamoto, I., Oiki, S.
Organizer
36^<th> International Congress of Physiological Sciences
Place of Presentation
Kyoto
Related Report
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[Presentation] Lidocaine inhibits voltage-gated proton channels in rat microglia2009
Author(s)
Matsuura, T., Mori, T., Kuno M., Hasaka, M., Kawawaki, J., Nishikawa, K, Sawada, M, Asada, A.
Organizer
36^<th> International Congress of Physiological Sciences
Place of Presentation
Kyoto
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