Analysis of bi-directional signal transduction elicited by adhesion between leukocyte and endothelial cells

• Project/Area Number: 20590214
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Osaka City University
• Principal Investigator: FUJITA Hisakazu Osaka City University, 大学院・医学研究科, 講師 (30212187)
• Co-Investigator(Kenkyū-buntansha): KATO Takayuki 大阪市立大学, 大学院・医学研究科, 講師 (50343413) ; KITAGAWA Seiichi 大阪市立大学, 大学院・医学研究科, 教授 (50133278)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞間接着 / カルパイン / 血管内皮細胞 / 白血球 / 細胞間接着分子 / 低分子量Gタンパク質

Research Abstract

1)We found the molecular interaction between JAML (Junctional adhesion molecule-like) and ESAM (Endothelial cell-selective adhesion molecule). 2) Potent calpain inhibitors stimulate cell functions via activation of human formyl peptide receptor and human formyl peptide receptor-like 1.

Research Products

• [Journal Article] Calpain inhibitors stimulate phagocyte functions via activation of human formyl peptide receptors (2011)
  • Author(s): Fujita, H.
  • Journal Title: Arch Biochem Biophys. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Granulocyte colony-stimulating factor negatively regulates Toll-like receptor agonist-induced cytokine production in human neutrophils. (2010)
  • Author(s): S. Fukuzono, T. Kato, H. Fujita, N. Watanabe, S. Kitagawa
  • Journal Title: Arch.Biochem.Biophys 495 Pages: 144-151
  • Peer Reviewed
• [Journal Article] Granulocyte colony-stimulating factor negatively regulates Toll-like receptor agonist-induced cytokine production in human neutrophils. (2010)
  • Author(s): Fukuzono, S.
  • Journal Title: Arch Biochem Biophys. Volume: 495 Pages: 144-151
  • Peer Reviewed
• [Journal Article] Granulocyte colony-stimulating factor negatively regulates Toll-like receptor agonist-induced cytokine production in human neutophils. (2010)
  • Author(s): Fukuzono, S
  • Journal Title: Arch Biochem Biophys. 495 Pages: 144-151
  • Peer Reviewed
• [Journal Article] Calpain inhibition induces activation of the distinct signalling pathways and cell migration in human monocytes. (2009)
  • Author(s): H. Noma, T. Kato, H. Fujita, M. Kitagawa, T. Yamano, S. Kitagawa
  • Journal Title: Immunology 128
  • Peer Reviewed
• [Journal Article] Calpain inhibition induces activation of the distinct signalling pathways and cell migration in human manocytes. (2009)
  • Author(s): Noma, H
  • Journal Title: Immunology 128
  • Peer Reviewed
• [Journal Article] Calpain inhibition induces activation of distinct signalling pathways and cell migration in human monocytes (2009)
  • Author(s): Noma, H.
  • Journal Title: Immunology (In press)
  • Peer Reviewed
• [Journal Article] Calpain inhibition delays neutrophil apoptosis via cyclic AMP-independent activation of protein kinase A and protein kinase A-mediated stabilization of Mcl-1 and X-linked inhibitor of apoptosis (XIAP). (2008)
  • Author(s): Y. Ozaki, T. Kato, M. Kitagawa, H. Fujita, S. Kitagawa
  • Journal Title: Arch Biochem Biophys. 477 Pages: 227-231
  • Peer Reviewed
• [Journal Article] Calpain-mediated regulation of the distinct signaling pathways and cell migration in human neutrophils. (2008)
  • Author(s): M. Katsube, T. Kato, M. Kitagawa, H. Noma, H. Fujita, S. Kitagawa
  • Journal Title: J.Leukoc.Biol. 84 Pages: 255-263
  • Peer Reviewed
• [Journal Article] Calcium Channel Blocker Suppress Cytokine-Induced Activation of Human Neutrophil. (2008)
  • Author(s): E. Shima, M. Katsube, T. Kato, M. Kitagawa, F. Hato, M. Hino, T. Takahashi, H. Fujita, S. Kitagawa
  • Journal Title: Am.J.Hypertension 21 Pages: 78-84
  • NAID: 130007039623
  • Peer Reviewed
• [Journal Article] Calpain inhibition delays neutrophil apoptosis via cyclic AMP-independent activation of protein kinase A and protein kinase A-mediated stabilization of Mcl-1 and X-linked inhibitor of apoptosis (XIAP) (2008)
  • Author(s): Ozaki, Y.
  • Journal Title: Arch. Biochem. and Biophys. 477 Pages: 227231-227231
  • Peer Reviewed
• [Journal Article] Calpain-mediated regulation of the distinct signaling Pathways and cell migration in human neutrophils (2008)
  • Author(s): Katsube, M.
  • Journal Title: J. of Leukoc. Biol. 84 Pages: 255263-255263
  • Peer Reviewed
• [Journal Article] Potent calpain inhibitors stimulate cell functions via activation of human formyl peptide receptor and human formyl peptide receptor-like 1
  • Author(s): H. Fujita, T. Kato, S Fukuzono, N. Watanabe, T. Takahashi, S. Kitagawa
  • Journal Title: Arch.Biochem.Biophys in press
  • Peer Reviewed
• [Presentation] Calpain inhibitors stimulate cell functions via activation of human formyl peptide receptor and formyl peptide receptor-like 1 (2010)
  • Author(s): 藤田寿一
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] G-CSFはTLRアゴニスト刺激によって誘導されるヒト好中球からのサイトカインの産生を負に制御する (2009)
  • Author(s): 福薗駿介
  • Organizer: 日本生理学会近畿生理談話会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-13
• [Remarks]
  • URL: http://www.med.osaka-cu.ac.jp/physiology2/