The regulation mechanisms of subcellular localization of ion channels in developing neurons.

Research Project

Project/Area Number	20590217
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	General physiology
Research Institution	Saitama Medical University
Principal Investigator	NAKAHIRA Kensuke Saitama Medical University, 医学部, 講師 (10260043)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	シナプス伝達 / 電位依存性Kチャネル / 小脳顆粒細胞 / 培養神経細胞 / 苔状線維
Research Abstract	Kv4.2, an A-type K+ channel, changed its subcellular localization from soma to the dendrites and synapses during synaptogenesis. In order to investigate the mechanisms of this regulation, we used EGFP-tagged-fusion channels. The fusion proteins were found mainly in ER when cDNA was transfected in culture cells. The Kv4.2 binding proteins, such as KChIP1, enhanced the surface expression of the channels by promoting translocation from ER to Golgi body.