Quantitative analyses of G protein activation by the single molecule imaging

• Project/Area Number: 20590224
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: TATEYAMA Michihiro National Institute for Physiological Sciences, 分子生理研究系, 准教授 (30276472)
• Co-Investigator(Renkei-kenkyūsha): KUBO Yoshihiro 生理学研究所, 分子生理研究系, 教授 (80211887)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 分子・細胞生理学 / FRET / 代謝型グルタミン酸受容体 / G蛋白質 / G蛋白質共役型受容体 / マルチシグナリング

Research Abstract

G protein coupled receptors (GPCR) play important roles in the inter-cellular signaling and their initial signaling processes were investigated by FRET. FRET values between the fluorescent proteins tethered to the receptors and subunits of GPCRs were measured in living cells. As for the family A Gq coupled receptors, agonist-induced receptor activation, the coupling between receptor and G proteins and the activation of G proteins were detected as ligand-induced FRET changes.

Research Products

• [Journal Article] G蛋白質共役型受容体の構造変化の全反射照明下FRET法による解析 (2010)
  • Author(s): 久保義弘、立山充博
  • Journal Title: 医学の歩み(医歯薬出版株式会社) 233巻第9号 Pages: 699-703
• [Journal Article] Ligand-induced rearrangements of the GABAB receptor revealed by fluorescence resonance energy transfer. (2010)
  • Author(s): Matsushita S., Nakata H., Kubo Y., Tateyama M.
  • Journal Title: J.Biol.Chem. 285 Pages: 10291-10299
  • Peer Reviewed
• [Journal Article] G蛋白質共役型受容体の構造変化の全反射照明下FRET法による解析 (2010)
  • Author(s): 久保義弘、立山充博
  • Journal Title: 医学の歩み 最新G蛋白質共役受容体研究 Volume: 233 Pages: 699-703
• [Journal Article] Ligand-induced rearrangements of the GABAB receptor revealed by fluorescence resonance energy transfer (2010)
  • Author(s): Matsushita, S., Nakata, H.Kubo, Y., Tateyama, M.
  • Journal Title: Journal of Biological Chemistry 285 Pages: 10291-10299
  • Peer Reviewed
• [Journal Article] Structural rearrangements of the motor protein prestin revealed by fluorescence resonance energy transfer. (2009)
  • Author(s): Gleitsman KR, Tateyama M., Kubo Y.
  • Journal Title: Am.J.Physiol.Cell Physiol. 297
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Kubo, Y., Tateyama M
  • Journal Title: Structural rearrangement and functional relation of the metabotropic glutamate receptor. in Handbook of Neurochemistry and Molecular Neurobiology.3rd Edition(Springer)
• [Journal Article] Regulatory role of C-terminus in the G protein coupling of the metabotropic glutamate receptor 1. (2008)
  • Author(s): 立山充博, 久保義弘
  • Journal Title: J.Neurochem. 107 Pages: 1036-1046
  • Peer Reviewed
• [Journal Article] Regulatory role of C-terminus in the G protein coupling of the metabotropic glutamate receptor 1 (2008)
  • Author(s): Tateyama, M., Kubo, Y.
  • Journal Title: Journal of Neurochemistry 107 Pages: 1036-1046
  • Peer Reviewed
• [Journal Article] The intra-molecular activation mechanisms of the dimeric metabotropic glutamate receptor 1 differ depending on the type of G proteins.
  • Author(s): 立山充博, 久保義弘
  • Journal Title: Neuropharmacol. (in press)
  • Peer Reviewed
• [Presentation] Ligand-induced structural rearrangements of G protein coupled receptors revealed by FRET analysis (2011)
  • Author(s): 立山充博、松下真一、久保義弘
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-22
• [Presentation] Ligand-induced structural rearrangements of G protein coupled receptors revealed by FRET analysis (2011)
  • Author(s): 立山充博、松下真一、久保義弘
  • Organizer: 第87回日本薬理学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)(大会は中止、紙面発表)
  • Year and Date: 2011-03-22
• [Presentation] FRET analysis revealed difference in the Gq coupling between m1 muscarinic receptor and metabotropic glutamate receptor1 (2010)
  • Author(s): 立山充博、久保義弘
  • Organizer: 第33回日本神経科学会大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-03
• [Presentation] Inter-subunit interaction regulates the multiple signaling of metabotropic glutamate receptor 1a (2010)
  • Author(s): 立山充博、久保義弘
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手)
  • Year and Date: 2010-05-11
• [Presentation] 代謝型グルタミン酸受容体の多様なシグナリングの調節機構シンポジウム「グルタミン酸受容体の新側面」 (2009)
  • Author(s): 立山充博、久保義弘
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] 代謝型グルタミン酸受容体の多様なシグナリングの調節機構 (2009)
  • Author(s): 立山充博、久保義弘
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-16
• [Presentation] Inetersubunit activation of metabotropic glutamate receptor 1a triggers Gq coupled signaling pathway, but not Gi/o one (2009)
  • Author(s): 立山充博、久保義弘
  • Organizer: 第36回国際生理学会大会
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2009-07-31
• [Presentation] 代謝型グルタミン酸受容体2量体間の Trans 型活性化はGq応答を起こすがGi/o応答は起こさない (2009)
  • Author(s): 立山充博, 久保義弘
  • Organizer: 日本薬理学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-03-17
• [Presentation] PKA依存性リン酸化による代謝型グルタミン酸受容体I型の機能修飾 (2008)
  • Author(s): 立山充博, 久保義弘
  • Organizer: 日本神経科学学会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-11
• [Book] Handbook of Neurochemistry and Molecular Neurobiology 3rd Edition, Structural rearrangement and functional relation of the metabotropic glutamate receptor (2009)
  • Author(s): Kubo Y., Tateyama M
  • Publisher: Springer