Quantitative analyses of G protein activation by the single molecule imaging
Project/Area Number |
20590224
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General physiology
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Research Institution | National Institute for Physiological Sciences |
Principal Investigator |
TATEYAMA Michihiro National Institute for Physiological Sciences, 分子生理研究系, 准教授 (30276472)
|
Co-Investigator(Renkei-kenkyūsha) |
KUBO Yoshihiro 生理学研究所, 分子生理研究系, 教授 (80211887)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 分子・細胞生理学 / FRET / 代謝型グルタミン酸受容体 / G蛋白質 / G蛋白質共役型受容体 / マルチシグナリング |
Research Abstract |
G protein coupled receptors (GPCR) play important roles in the inter-cellular signaling and their initial signaling processes were investigated by FRET. FRET values between the fluorescent proteins tethered to the receptors and subunits of GPCRs were measured in living cells. As for the family A Gq coupled receptors, agonist-induced receptor activation, the coupling between receptor and G proteins and the activation of G proteins were detected as ligand-induced FRET changes.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article]2009
Author(s)
Kubo, Y., Tateyama M
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Journal Title
Structural rearrangement and functional relation of the metabotropic glutamate receptor. in Handbook of Neurochemistry and Molecular Neurobiology.3rd Edition(Springer)
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