Anxiety is involved in tonic GABAergic inhibition in cerebral cortex and limbic system

• Project/Area Number: 20590229
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Hirosaki University
• Principal Investigator: YAMADA Junko 弘前大学, 大学院・医学研究科, 講師 (30334965)
• Co-Investigator(Renkei-kenkyūsha): UENO Shinya 弘前大学, 大学院・医学研究科, 教授 (00312158)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: GABA / 抑制性シナプス伝達 / 不安 / ニューロン / トニックシグナル / 扁桃体 / tonic current / 卵巣切除 / スライスパッチクランプ / ステロイドホルモン / PRIP-1ノックアウトマウス / トニック / ホルモン

Research Abstract

PRIP1, a novel IP3 binding protein, functioned as a transporter protein of GABAA receptor to plasma membrane. Then PRIP1-KO mice should be changed the GABAergic inhibitory system. KO mice showed decreasing motor activity by an open-field test. Using brain slices and whole-cell patch-clamp recordings, there are no significant difference both amplitude and frequency of mEPSCs and mIPSCS between WT and KO mice. Tonic GABAergic inhibition exist in principal neurons of the basolateral amygdala in WT mice but PRIP-1 KO mice have imperfection of this inhibitory system.

Research Products

• [Journal Article] Dysfunction of extrasynaptic GABAergic transmission in PRIP-1 KO mice is associated with an epilepsy phenotype (2011)
  • Author(s): Zhu G., Yoshida S., Migita K., Yamada J., Ueno S
  • Journal Title: J Pharmacol Exp Ther Volume: 340(3) Issue: 3 Pages: 520-8
  • DOI: 10.1124/jpet.111.182386
  • URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22128345
  • Peer Reviewed
• [Journal Article] Phenotypes of pain behavior in phospholipase C-related but catalytically inactive protein type 1 knockout mice (2011)
  • Author(s): Migita K., Tomiyama M., Yamada J., Fukuzawa M., Kanematsu T., Hirata M., Ueno S
  • Journal Title: Molecular pain Volume: 7 Pages: 79-79
  • DOI: 10.1186/1744-8069-7-79
  • URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22008183
  • Peer Reviewed
• [Journal Article] A low-concentration tributyltin deteriorates inhibitory synaptogenesis and induces neuronal death in immature but not mature neurons (2010)
  • Author(s): Yamada J., Inoue K., Furukawa T., Fukuda A
  • Journal Title: Toxicol. Lett Volume: 198(2) Pages: 282-288
  • URL: http://dx.doi.org/10.1016/j.toxlet.2010.07.011
  • Peer Reviewed
• [Journal Article] A low-concentration tributyltin deteriorates inhibitory synaptogenesis and induces neuronal death in immature but not mature neurons (2010)
  • Author(s): Yamada J., et al
  • Journal Title: Toxicology letters Volume: 198 Pages: 282-288
  • Peer Reviewed
• [Journal Article] Involvement of P2X2 and P2X3 receptors in dorsal root ganglion neurons of streptozotocin-induced diabetic neuropathy (2010)
  • Author(s): Migita K., Honda K., Yamada J., et al
  • Journal Title: Hirosaki Medical Journal Volume: 61(Suppl.)
  • Peer Reviewed
• [Journal Article] Compensatory enhancement of intrinsic spiking upon NKCC1 disruption in neonatal hippocampus (2009)
  • Author(s): Sipila S., Huttu K., Yamada J., et al.
  • Journal Title: The Journal of Neuroscience 29(21) Pages: 6982-8
  • Peer Reviewed
• [Journal Article] GABAergic depolarization of the axon initial segment in cortical principal neurons is caused by the Na-K-2Cl cotransporter NKCC1 (2008)
  • Author(s): Khirug S., Yamada J., Afzalov R., Voipio J., Khiroug L., Kaila K
  • Journal Title: J. Neurosci Volume: 28(18) Issue: 18 Pages: 4635-39
  • DOI: 10.1523/jneurosci.0908-08.2008
  • Peer Reviewed
• [Journal Article] GABAergic depolarization of the axon initial segment in cortical princinal neurons is caused by the Na-K-2C1 cotransporter NKCC1 (2008)
  • Author(s): Stanislav Khirug, Junko Yamada et.al.
  • Journal Title: The Journal of Neuroscience 28(18) Pages: 4635-39
  • Peer Reviewed
• [Presentation] PRIP1 is involved in tonic GABAergic transmission in BLA neurons (2011)
  • Author(s): J.Yamada, K.Migita, T.Kanematsu, M Hirata, S.Ueno
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: Yokohama(中止のため紙上発表)
  • Year and Date: 2011-03-28
• [Presentation] Potential developmental regulation of neuronal KCC2 activity by taurine (2011)
  • Author(s): K.Inoue, T Furukawa, J Yamada, et al
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: Yokohama(中止のため紙上発表)
  • Year and Date: 2011-03-28
• [Presentation] Alteration of tonic GABAergic inhibition of BLA neurons in PRIP1-KO mice (2010)
  • Author(s): J.Yamada, K.Migita, T.Kanematsu, M Hirata, S.Ueno
  • Organizer: Hirosaki International Forum2010
  • Place of Presentation: Hirosaki
  • Year and Date: 2010-10-29
• [Presentation] Alteration of tonic GABAergic inhibition of BLA neurons in PRIP1-KO mice (2010)
  • Author(s): Junko Yamada
  • Organizer: Hirosaki International Forum
  • Place of Presentation: Hirosaki
• [Presentation] Pharmacological properties of tonic GABAergic inhibition in the amygdala neurons of PRIP1-KO mice (2009)
  • Author(s): Junko Yamada
  • Organizer: Neuroscience 2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-19
• [Presentation] Pharmacological properties of tonic GABAergic inhibition in the amygdala neurons of PRIP1-KO mice (2009)
  • Author(s): Junko Yamada, et al.
  • Organizer: IUPS2009
  • Place of Presentation: シカゴMcCormick Place
  • Year and Date: 2009-10-19
• [Presentation] ltered tonic GABAergic inhibition of amygdala neurons in PRIP1-KO mice (2009)
  • Author(s): Junko Yamada, et al.
  • Organizer: Neuro2009
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] Downregulation of GABA_A receptor-mediated tonic inhibition in the basolateral amygdala of PRIP-1 KO mice (2009)
  • Author(s): Junko Yamada, et al.
  • Organizer: IUPS2009
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-29
• [Presentation] GABAergic depolarization of the axon initial segment in cortical orincipal neurons is mediated by NKCC1 (2009)
  • Author(s): Junko Yamada, et.al.
  • Organizer: Hirosaki International Forum
  • Place of Presentation: 弘前大学
  • Year and Date: 2009-03-27
• [Presentation] PRIP-1 is involved in GABA_A receptor-mediated tonic inhibition in basolateral amygdala (2008)
  • Author(s): Junko Yamada
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washingtong DC
  • Year and Date: 2008-11-16
• [Presentation] PRIP-I is involved in GABA_A receptor-mediated tonic inhibition in basolateral (2008)
  • Author(s): Junko Yamada, et.al.
  • Organizer: Neuroscience 2008
  • Place of Presentation: ワシントンDC(米国)
  • Year and Date: 2008-11-16
• [Presentation] GABAergic depolarization of the axon initial segment is caused by NKCC1 in cortical principal neurons (2008)
  • Author(s): Junko Yamada, et.al.
  • Organizer: Neuroscience 2008
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-10