Clinical application of Nesfatin-1 to obesity from the point of the suppression of adipogenesis.
Project/Area Number |
20590247
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General pharmacology
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Research Institution | Gunma University |
Principal Investigator |
OKADA Shuichi Gunma University, 医学部, 講師 (20260474)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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Keywords | 肥満 / 脂肪分化 / 脂肪細胞分化 / Nucleobindin-2 / 生理活性 / シグナル伝達 / 生体分子 / 細胞・組織 / 動物 / 摂食 / ホルモン |
Research Abstract |
Nucleobindin-2 is a 396 amino acid EF-hand Ca^<2+> binding protein that can be further processed to generate an 82 amino terminal peptide termed Nesfatin-1. To examine the function of Nucleobindin-2 in adipocyte differentiation, culutred 3T3-L1 adipogenesis were incubated with various concentrations of GST, GST-Nucleobindin-2, GST-DNesfatin-1 or GST-Nesfatin-1 prior to and during the initiation of adipocyte differentiation. Nucleobindin-2 treatment at physiological concentrations decreased neutral lipid accumulation (Oil-Red O staining) and expression of several marker genes for adipocyte differentiation (PPAR・, aP2, and adipsin). In contrast, deletion of the Nesfatin-1 domain in Nucleobindin-2 (DNesfatin-1) had no significant effect on 3T3-L1 adipocyte differentiation. Although Nesfatin-1 treatment alone also reduced 3T3-L1 adipocyte differentiation and expression of marker genes, this required super physiologic concentrations. Moreover, 3T3-L1 cells stably over expresseing Nuclobindin-2 displayed a significant reduction of adipocyte differentiation. In contrast, reduced expression of Nucleobindin-2 by shRNA resulted in a marked increase of adipocyte differentiation. Taken together, these data indicate that Nucleobindin-2 is secreted from preadipocytes and functions as a suppressor of adipocyte differentiation.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Peripheral administration of nesfatin-1 reduces food intake in mice : the leptin-independent mechanism.2009
Author(s)
Hiroyuki Shimizu, Shinsuke Oh-I, Koshi Hashimoto, Masanori Nakata, Sawako Yamamoto, Natsu Yoshida, Hiroshi Eguchi, Ikuo Kato, Kinji Inoue, Tetsurou Satoh, Shuichi Okada, Masanobu Yamada, Toshihiko Yada, Masatomo Mori
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Journal Title
Endocrinology. 150
Pages: 662-71
Related Report
Peer Reviewed
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