Development of novel therapeutic approach for aneurysm using decoy oligodeoxynucleotide with a ribbon-shaped circular structure.
| Project/Area Number |
20590251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
MIYAKE Takashi Osaka University, 連合小児発達学研究科, 寄附講座特任助教 (40219746)
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| Co-Investigator(Kenkyū-buntansha) |
TANIYAMA Yoshiaki 大阪大学, 大学院・医学系研究科, 寄附講座准教授 (60372611)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 核酸医薬 / 動脈瘤 / デコイ |
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| Research Abstract |
We modified decoy oligodeoxynucleotide (ODN) against NFKB and ets, to a ribbon-shaped circular structure, to increase its stability for systemic administration. Intraperitoneal administration of ribbon-type decoy ODNs (R-ODNs) was performed in an elastase-induced rat aneurysm model. Treatment with chimeric R-ODN significantly inhibited aortic dilatation accompanied by a reduction of secretion of several proteases from macrophages. In contrast, conventional phosphorothioate decoy ODN failed to prevent aneurysm formation. In addition, we investigated the effect of intravenous injection of modified R-ODN against NFKB in a mouse aneurysm model. Treatment with modified R-ODN showed a tendency to decrease the size of AAA. Further modification of the decoy strategy would provide a means of less invasive molecular therapy for human AAA.
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Report
(4 results)
Research Products
(12 results)
