| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Membrane-associated prostaglandin E_2 synthase (mPGES1) is an inducible terminal enzyme in the biosynthetic pathway for prostaglandin E_2, which participates in many biological processes such as neovascularization. We have already shown that Egr-1 is a key transcription factor in regulating the inducible expression of mPGES. In this study I have investigated the role of NAB2, a specific corepressor of EGR-1, to down-regulate growth factor-induced mPGES expression in inflammatory cells and to inhibit neovascularization. NAB2 was rapidly induced by increased levels of Egr-1 in vitro and in vivo model of neovascularization. Moreover, it was observed that the induction of mPGES1 gene expression and production of PGE_2 by treatment of siRNA specific for NAB2. These results suggest that NAB2 is involved in the regulation of the expression of mPGES1, and it may serve as a key molecule in the development and progression of neovascularization.
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