A novel mechanism of glutathione synthesis in neuron
Project/Area Number |
20590261
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General pharmacology
|
Research Institution | Teikyo University |
Principal Investigator |
AOYAMA Koji Teikyo University, 医学部, 講師 (00420943)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | グルタチオン / GTRAP3-18 / EAAC1 / 神経変性疾患 |
Research Abstract |
EAAC1 plays an important role in neuronal GSH synthesis. GTRAP3-18 is an EAAC1-binding protein to inhibit the function in neurons. However, it is still elusive that GTRAP3-18 negatively regulates neuronal GSH synthesis in vivo. In this study, we showed that intraventricular injection of GTRAP3-18 siRNA decreased GSHcontent in the hippocampus, while intraventricular injection of methyl β-cyclodextrin decreased GSH content with increased GTRAP3-18 expression in the hippocampus. These results suggest that GTRAP3-18 negatively regulates neuronal GSH synthesis in the hippocampus.
|
Report
(4 results)
Research Products
(15 results)