Regulation of expression of vascular cation channels by transcriptional and epigenetical modulation
| Project/Area Number |
20590263
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
MURAKI Katsuhiko Aichi Gakuin University, 薬学部, 教授 (20254310)
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| Co-Investigator(Kenkyū-buntansha) |
HATANO Noriyuki 愛知学院大学, 薬学部, 講師 (50454319)
ITOH Yuka 名古屋市立大学, 大学院・薬学研究科, 助教 (40454326)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | カチオンチャネル / エピジェネティクス / 転写 / TRP / ステロイド / ヒストン / 転写制御 / イオンチャネル / メチル化 / アセチル化 |
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| Research Abstract |
Change of ion channel expression by transcriptional and epigenetical modulation plays a pivotal role in development of human diseases. We thus investigated regulation of expression of a non-selective cation channel, nicotinic ACh receptor α7 (nAChRα7) in human synovial fibroblasts (HSFs). Analysis of expression of nAChRα7 mRNA transcripts between HSFs with or without rheumatoid arthritis (RA) revealed that nAChRα7 was lower in normal HSFs. Promoter of nAChRα7 gene was methylated only in normal HSFs. The expression of nAChRα7 mRNA transcripts was increased when the methylation was inhibited by treatment of normal HSFs with 5-aza-CdR. In HFSs with RA, expression of nAChRα7 is increased due to inhibition of methylation of nAChRα7 gene.
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Report
(4 results)
Research Products
(33 results)
