Dynamics of TIP60 histone acetyltransferase complex in DNA damage response

• Project/Area Number: 20590272
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: IKURA Tsuyoshi Tohoku University, 放射線生物研究センター, 准教授 (70335686)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: DNA損傷応答 / TIP60ヒストンアセチル化酵素 / ヒストンH2AX / アセチル化 / ユビキチン化 / ブロモドメイン / 機能的蛋白質複合体 / TIP60ヒストンアセチル化酵素複合体 / ユビキチン結合酵素UBC13 / DNA損傷応答シグナル / ヒストンのアセチル化 / ブロモドメイン蛋白質 / ヒストンシャペロン / 放射線感受性 / MS解析 / 蛋白質複合体

Research Abstract

Dynamics of TIP60 histone acetyltransferase complex in DNA damage response
To elucidate the role of the TIP60 histone acetyltransferase complex in DNA damage response, we analyzed the component of TIP60 complex by MS analysis. We identified the p120 bromodomain protein in the TIP60 complex. We found that p120 binds the acetylated histone H2AX in vitro and is required for the recruitment of UBC13 to damaged chromatin.

Research Products

• [Journal Article] Methionine Adenosyltransferase II Serves As a Transcriptional Corepressor of Maf Oncoprotein. (2011)
  • Author(s): Katoh, Y, Ikura, T., Hoshikawa, Y., Tashiro, S., Ohta, M., Kera, Y., Noda, T., Igarashi, K.
  • Journal Title: Mol Cell. 41
  • Peer Reviewed
• [Journal Article] Methionine Adenosyltransferase II Serves As a Transcriptional Corepressor of Maf Oncoprotein (2011)
  • Author(s): Katoh Y
  • Journal Title: Mol.Cell Volume: 41 Pages: 554-566
  • Peer Reviewed
• [Journal Article] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot. (2010)
  • Author(s): Sun, J., Oma, Y., Harata, M., Kono, K., Shima, H., Kinomura, A., Ikura, T., Mizutani, S., Kanaar, R., Tashiro, S.
  • Journal Title: PloS ONE 5
  • NAID: 120002737822
  • Peer Reviewed
• [Journal Article] Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase. (2010)
  • Author(s): Niida H, Katsuno Y, Sengoku M, Shimada M, Yukawa M, Ikura M, Ikura T, Kohno K, Shima H, Suzuki H, Tashiro S, Nakanishi M.
  • Journal Title: Genes Dev. 24 Pages: 333-338
  • NAID: 130007000033
  • Peer Reviewed
• [Journal Article] Proline cis/trans isomerase Pin1 regulates peroxisome proliferator-activated receptor {gamma} activity through the direct binding to the AF-1 domain. (2010)
  • Author(s): Fujimoto Y, Shiraki T, Horiuchi Y, Waku T, Shigenaga A, Otaka A, Ikura T, Igarashi K, Aimoto S, Tate SI, Morikawa K.
  • Journal Title: J Biol Chem. 285 Pages: 3126-32
  • Peer Reviewed
• [Journal Article] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot (2010)
  • Author(s): Sun J
  • Journal Title: PLoS ONE Volume: 5
  • Peer Reviewed
• [Journal Article] Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase. (2010)
  • Author(s): Niida H
  • Journal Title: Genes Dev Vol.24 Pages: 333-338
  • Peer Reviewed
• [Journal Article] Proline cis/trans isomerase Pinl regulates peroxisome proliferator-activated receptor {gamma} activity through the direct binding to the AF-1 domain. (2010)
  • Author(s): Fujimoto Y
  • Journal Title: J Biol Chem Vol.285 Pages: 3126-3132
  • Peer Reviewed
• [Journal Article] ユビキチン化とDNA修復 (2010)
  • Author(s): 井倉正枝
  • Journal Title: G. I. Research Vol.18 Pages: 107-114
• [Journal Article] The human actin-related protein hArp5 : nucleo-cytoplasmic shuttling and involvement in DNA repair (2009)
  • Author(s): Kitayama K, Kamo M, Oma Y, Matsuda R, Uchida T, Ikura T, Tashiro S, Ohyama, T, Winsor B, Harata M.
  • Journal Title: Exp.CellRes. 15 Pages: 206-217
  • Peer Reviewed
• [Journal Article] The human actin-related protein hArp5 : nucleo-cytoplasmic shuttling and involvement in DNA repair (2009)
  • Author(s): Kitayama K
  • Journal Title: Exp. Cell Res Vol.15 Pages: 206-217
  • Peer Reviewed
• [Journal Article] ゲノムストレス応答と核内蛋白質の高次構造体構築 (2009)
  • Author(s): 田代聡
  • Journal Title: 実験医学増刊号 Vol.27 Pages: 173-178
• [Journal Article] 放射線応答とクロマチンダイナミクス (2009)
  • Author(s): 井倉毅
  • Journal Title: 放射線生物研究 Vol.44 Pages: 1-10
  • NAID: 40016597006
• [Journal Article] ヒストンH2AXのアセチル化とユビキチン化によって制御される放射線誘発DNA損傷初期応答シグナルの解明 (2009)
  • Author(s): 井倉毅
  • Journal Title: 放影協ニュース Vol.61 Pages: 12-14
• [Journal Article] Bach1 inhibits oxidative stress-induced cellular senescence by impeding p53 function on chromatin. (2008)
  • Author(s): Dohi, Y, Ikura, T, Hoshikawa, Y, Katoh, Y, Ota, K, Nakanome, A., Muto, A., Omura, S,. Ohta, T., Ito, A., Yoshida, M., Noda, T., Igarashi, K.
  • Journal Title: Nature Structural & Molecular Biology 15 Pages: 1246-1254
  • Peer Reviewed
• [Journal Article] Absence of Ku70 Gene Obliterates X-Ray-Induced lacZ Mutagenesis of Small Deletions in Mouse Tissues. (2008)
  • Author(s): Uehara, Y., Ikehata, H., Komura, J., Ito, A., Ogata, M ., Itoh, T., Hirayama, R., Furusawa, Y., Ando, K., Paunesku, T., Gayle, E., Woloschak, G.E., Komatsu, K., Matsuura, S., Ikura, T., Kamiya, K., Ono, T.
  • Journal Title: RADIATION RESEARCH 170 Pages: 216-223
  • Peer Reviewed
• [Journal Article] Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone crosstalk with H3K4 di- and tri-methylation. (2008)
  • Author(s): Nakagawa, T., Kajitani, T., Togo, S., Masuko, N., Ohdan, H., Hishikawa, Y., Koji T., Matsuyama, T., Ikura T., Muramatsu, M., Ito, T.
  • Journal Title: Genes Dev. 22 Pages: 37-49
  • Peer Reviewed
• [Journal Article] Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone crosstalk with H3K4 di- and tri-methylation. (2008)
  • Author(s): Nakagawa, T
  • Journal Title: Genes and Development 22 Pages: 37-49
  • Peer Reviewed
• [Journal Article] Absence of Ku70 Gene Obliterates X-Ray-Induced lacZ Mutagenesis of Small Deletions in Mouse Tissues (2008)
  • Author(s): Uehara, Y
  • Journal Title: RADIATION RESEARCH 170 Pages: 216-223
  • Peer Reviewed
• [Journal Article] Bachl inhibits oxidative stress-induced cellular senescence by impeding p53 function on chromatin (2008)
  • Author(s): Dohi, Y
  • Journal Title: Nature Structural & Molecular Biology 15 Pages: 1246-1254
  • Peer Reviewed
• [Journal Article] The human actin-related protein hArp5 : nucleo-cytoplasmic shuttling an d involvement in DNA repair (2008)
  • Author(s): Kitayama K
  • Journal Title: Exp. Cell Res 315 Pages: 206-217
  • Peer Reviewed
• [Journal Article] 染色体境界領域の構造と機能 (2008)
  • Author(s): 井倉毅
  • Journal Title: 転写制御とエピジェネティクス 1 Pages: 29-33
• [Journal Article] クロマチン動態制御と発癌 (2008)
  • Author(s): 井倉正枝
  • Journal Title: 呼吸 27 Pages: 454-457
• [Presentation] DNA損傷応答シグナルにおけるヒストンH2AXのダイナミクス (2011)
  • Author(s): 井倉毅
  • Organizer: 第7回宮崎サイエンスキャンプ
  • Place of Presentation: 宮崎市
  • Year and Date: 2011-02-26
• [Presentation] DNA損傷初期シグナルにおけるヒストンシグナルネットワークの解明 (2010)
  • Author(s): 井倉毅
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-07
• [Presentation] The role of chromatin dynamics in DNA damage-induced checkpoint activation. (2010)
  • Author(s): 井倉毅
  • Organizer: 日本環境変異原学会第39回大会
  • Place of Presentation: つくば市
  • Year and Date: 2010-11-17
• [Presentation] The role of chromatin dynamics in DNA damage induced checkpoint activation. (2010)
  • Author(s): 井倉毅
  • Organizer: 日本環境変異原学会第39回大会
  • Place of Presentation: つくば市
  • Year and Date: 2010-11-17
• [Presentation] DNA損傷応答におけるヒストンH2AXのダイナミクスとその意義 (2010)
  • Author(s): 井倉毅
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-21
• [Presentation] Checkpoint activation regulated by DNA damage-induced H2AX eviction (2010)
  • Author(s): 井倉毅
  • Organizer: 56^<th> Annual Meeting Radiation Research Society
  • Place of Presentation: Hawaii
  • Year and Date: 2010-09-29
• [Presentation] Checkpoint activation regulated by DNA damage induced H2AX eviction (2010)
  • Author(s): 井倉毅
  • Organizer: 56^<th> Annual Meeting Radiation Research Society
  • Place of Presentation: Hawaii
  • Year and Date: 2010-09-29
• [Presentation] 損傷クロマチンの動的変化がもたらすDNA損傷応答シグナルの活性化メカニズム文部省化学研究費補助金 (2010)
  • Author(s): 井倉毅
  • Organizer: 特定領域研究「染色体サイクルの制御ネットワーク」最終領域会議
  • Place of Presentation: 鹿児島県霧島市
  • Year and Date: 2010-03-02
• [Presentation] 損傷クロマチンの動的変化がもたらすDNA損傷応答シグナルの活性化メカニズム (2010)
  • Author(s): 井倉毅
  • Organizer: 文部省化学研究費補助金、特定領域研究「染色体サイクルの制御ネットワーク」最終領域会議
  • Place of Presentation: 鹿児島県霧島市
  • Year and Date: 2010-03-02
• [Presentation] The role of chromatin dynamics in DNA damage-induced checkpoint activation (2010)
  • Author(s): 井倉毅
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010.
  • Place of Presentation: 長崎市
  • Year and Date: 2010-02-19
• [Presentation] DNA損傷応答におけるヒストン化学修飾の役割 (2009)
  • Author(s): 井倉毅
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-12
• [Presentation] Checkpoint activation regulated by DNA damage-induced H2AX eviction (2009)
  • Author(s): 井倉毅
  • Organizer: The 25^<th> Radiation Biology Center International Symposium.
  • Place of Presentation: 京都市Kyoto university
  • Year and Date: 2009-11-30
• [Presentation] Checkpoint activation regulated by DNA damage-induced H2AX eviction (2009)
  • Author(s): 井倉毅
  • Organizer: The 25^<th> Radiation Biology Center International Symposium, Kyoto university
  • Place of Presentation: 京都市
  • Year and Date: 2009-11-30
• [Presentation] The role of histone modification in DNA damage response (2009)
  • Author(s): 井倉毅
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-13
• [Presentation] The role of histone modification in DNA damage response (2009)
  • Author(s): 井倉毅
  • Organizer: 日本放射線影響学会 第52回大会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-13
• [Presentation] DNA損傷応答におけるクロマチン構造変換の分子機構とその役割 (2009)
  • Author(s): 井倉毅
  • Organizer: 生命科学複合研究教育センター講演会
  • Place of Presentation: 福井市福井大学
  • Year and Date: 2009-09-04
• [Presentation] DNA損傷応答におけるクロマチン構造変換の分子機構とその役割 (2009)
  • Author(s): 井倉毅
  • Organizer: 生命科学複合研究教育センター 講演会 福井大学
  • Place of Presentation: 福井市
  • Year and Date: 2009-09-04
• [Presentation] DNA損傷応答におけるクロマチン構造変換の分子機構とその役割文部省化学研究費補助金、特定領域研究「染色体サイクルの制御ネットワーク」 (2009)
  • Author(s): 井倉毅
  • Organizer: 第5回領域会議
  • Place of Presentation: 静岡市
  • Year and Date: 2009-06-16
• [Presentation] DNA損傷応答におけるクロマチン構造変換の分子機構とその役割 (2009)
  • Author(s): 井倉毅
  • Organizer: 文部省化学研究費補助金、特定領域研究「染色体サイクルの制御ネットワーク」第5回領域会議
  • Place of Presentation: 静岡市
  • Year and Date: 2009-06-16
• [Presentation] The role of chromatin dynamics in DNA damage-induced checkpoint activation (2009)
  • Author(s): 井倉毅
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010
  • Place of Presentation: 長崎市
  • Year and Date: 2009-02-19
• [Presentation] DNA損傷応答におけるヒストンコードの解明 (2008)
  • Author(s): 井倉毅
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-09
• [Presentation] DNA損傷応答におけるヒストンコードの解明 (2008)
  • Author(s): 井倉毅
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] DNA損傷応答におけるクロマチンダイナミクス弘前大学 (2008)
  • Author(s): 井倉毅
  • Organizer: 第13回遺伝子実験施設シンポジウム
  • Place of Presentation: 青森市
  • Year and Date: 2008-06-20
• [Presentation] DNA損傷応答におけるクロマチンダイナミクス (2008)
  • Author(s): 井倉毅
  • Organizer: 弘前大学、第13回遺伝子実験施設シンポジウム
  • Place of Presentation: 青森
  • Year and Date: 2008-06-20