The regulation of actin assembly during myofibrillogenesis

• Project/Area Number: 20590285
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: TAKEYA Ryu 九州大学, 医学研究院, 講師 (50335981)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞医化学 / 細胞・組織 / シグナル伝達

Research Abstract

In striated muscle, this actin filaments and thick filaments of myosin are highly organized to form myofibrils. However, little is known about the mechanism for organization of actin filaments in the contractile unit sarcomere. In the present study, we show that the mammalian formin Fhod3 regulates actin assembly and sarcomere organization in striated muscles.

Research Products

• [Journal Article] Expression and subcellular localization of mammalian formin Fhod3 in the embryonic and adult heart (2012)
  • Author(s): Kan-o M, Takeya R*, Taniguchi K, Tanoue Y, Tominaga R, Sumimoto H
  • Journal Title: PLoS ONE Volume: 7
  • Peer Reviewed
• [Journal Article] A conserved region between the TPR and activation domains of p67 phox participates in activation of the phagocyte NADPH oxidase (2010)
  • Author(s): Maehara Y, Miyano K, Yuzawa S, Akimoto R, Takeya R, Sumimoto H
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 31435-31445
  • Peer Reviewed
• [Journal Article] A conserved region between the TPR and activation domains of p67phox participates in activation of the phagocyte NADPH oxidase. (2010)
  • Author(s): Maehara Y, Miyano K, Yuzawa S, Akimoto R, Takeya R, Sumimoto H.
  • Journal Title: J.Biol.Chem. 285 Pages: 31435-31445
• [Journal Article] A conserved region between the TPR and activation domains of p67^<phox> participates in activation of the phagocyte NADPH oxidase. (2010)
  • Author(s): Maehara Y, Miyano K, Yuzawa S, Akimoto R, Takeya R, Sumimoto H.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 31435-31445
  • Peer Reviewed
• [Journal Article] A region N-terminal to the tandem SH3 domain of p47 phox plays a crucial role in the activation of the phagocyte NADPH oxidase (2009)
  • Author(s): Taura M, Miyano K, Minakami R, Kamakura S, Takeya R, Sumimoto H
  • Journal Title: Biochem. J Volume: 419 Pages: 329-338
  • Peer Reviewed
• [Journal Article] The mammalian formin Fhod3 regulates actin assembly and sarcomere organization in striated muscles (2009)
  • Author(s): Taniguchi K, Takeya R, Suetsugu S, Kan-o M, Narusawa M, Shiose A, Tominaga R, Sumimoto H.
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 29873-29881
  • Peer Reviewed
• [Journal Article] Mammalian formin Fhod3 regulates actin assembly and sarcomere organization in striated muscles. (2009)
  • Author(s): Taniguchi K, Takeya R, Suetsugu S, Kan-OM, Narusawa M, Shiose A, Tominaga R, Sumimoto H.
  • Journal Title: J.Biol.Chem. 284 Pages: 29873-29881
• [Journal Article] A region N-terminal to the tandem SH3 domain of p47phox plays a crucial role in the activation of the phagocyte NADPH oxidase. (2009)
  • Author(s): Taura M, Miyano K, Minakami R, Kamakura S, Takeya R, Sumimoto H.
  • Journal Title: Biochem.J. 419 Pages: 329-338
• [Journal Article] Mammalian formin Fhod3 regulates actin assembly and sarcomere organization in striated muscles (2009)
  • Author(s): Taniguchi, K., Takeya, R., Suetsugu, S., Kan-o, M., Narusawa, M., Shiose, A., Tominaga, R., Sumimoto, H.
  • Journal Title: J.Biol.Chem. 284 Pages: 29873-29881
  • Peer Reviewed
• [Journal Article] A region N-terminal to the tandem SH3 domain of p47phox plays a crucialrole in activation of the phagocyte NADPH oxidase (2009)
  • Author(s): Taura M, Miyano K, Minakami R, Kamakura S, Takeya R, Sumimoto H.
  • Journal Title: Biochem J. 419 Pages: 329-338
  • Peer Reviewed
• [Journal Article] A region N-terminal to the tandem SH3 domain of p47phox plays a crucialrole in the activation of the phagocyte NADPH oxidase. (2009)
  • Author(s): Taura M, Miyano K, Minakami R, Kamakura S, Takeya R, Sumimoto H.
  • Journal Title: Biochem J. 419 Pages: 329-338
  • Peer Reviewed
• [Journal Article] The mammalian formin FHOD1 is activated through phosphorylation by ROCK and mediates thrombin-induced stress fiber formation in endothelial cells (2008)
  • Author(s): Takeya R, Taniguchi K, Narumiya S, Sumimoto H
  • Journal Title: EMBO J Volume: 27 Pages: 618-628
  • Peer Reviewed
• [Journal Article] Enhanced expression of NADPH oxidase Nox4 in human gliomas and its roles in cell proliferation and survival. (2008)
  • Author(s): Shono T, Yokoyama N, Uesaka T, Kuroda J, Takeya R, Yamasaki T, Amano T, Mizoguchi M, Suzuki SO, Niiro H, Miyamoto K, Akashi K, Iwaki T, Sumimoto H, Sasaki T.
  • Journal Title: Int. J. Cancer Volume: 123 Pages: 787-792
  • Peer Reviewed
• [Journal Article] Enhanced expression of NADPH oxidase Nox4 in human gliomas and its roles in cell proliferation and survival. (2008)
  • Author(s): Shono T, Yokoyama N, Uesaka T, Kuroda J, Takeya R, Yamasaki T, Amano T, Mizoguchi M, Suzuki SO, Niiro H, Miyamoto K, Akashi K, Iwaki T, Sumimoto H, Sasaki T
  • Journal Title: Int.J. Cancer 123 Pages: 787-792
• [Journal Article] The mammalian formin FHOD1 is activated through phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells. (2008)
  • Author(s): Takeya R, Taniguchi K, Narumiya S, Sumimoto H.
  • Journal Title: EMBO J. 27 Pages: 618-628
• [Journal Article] Enhanced expression of NADPH oxidase Nox4 in human gliomas and its roles in cell proliferation and survival. (2008)
  • Author(s): Shono T, Yokoyama N, Uesaka T, Kuroda J, Takeya R, Yamasaki T, Amano T, Mizoguchi M, Suzuki SO, Niiro H, Miyamoto K, Akashi K, Iwaki T, Sumimoto H, Sasaki T.
  • Journal Title: Int J Cancer 123 Pages: 787-792
  • Peer Reviewed
• [Presentation] The essential role of mammalian formin FHOD3 in sarcomere organization during heart development (2012)
  • Author(s): Ryu Takeya, Meikun Kan-o, Takaya Abe, Naoyuki Kitajima, Motohiro Nishida, Ryuji Tominaga, Hitoshi Kurose, Hideki Sumimoto.
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
• [Presentation] formin相同蛋白質Fhod3のマウス胎仔、成獣、およびヒト心筋サルコメアにおける局在 (2011)
  • Author(s): 神尾明君,武谷立,田ノ上禎久,富永隆治,住本英樹.
  • Organizer: 第84回日本生化学大会
  • Place of Presentation: 横浜
• [Presentation] formin相同蛋白質Fhod3の心筋サルコメアにおける局在と機能 (2011)
  • Author(s): 神尾明君,武谷立,田ノ上禎久,富永隆治,住本英樹.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] formin相同蛋白質FHOD3の筋細胞における生理的役割 (2009)
  • Author(s): 武谷立,谷口賢一郎,住本英樹
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Place of Presentation: 福岡
• [Presentation] formin相同蛋白質FHOD3の活性制御機構の解明 (2009)
  • Author(s): 成澤慈,武谷立,住本英樹
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Place of Presentation: 福岡
• [Presentation] Regulation of actin assembly and sarcomere organization in cardiomyocytes by the formin homology protein Fhod3. (2009)
  • Author(s): 谷口賢一郎,武谷立,末次志郎,神尾明君,成澤慈,塩瀬明,富永隆治,住本英樹.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] formin 相同蛋白質FHOD3の活性制御機構の解明 (2009)
  • Author(s): 成澤慈, 武谷立, 住本英樹
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Place of Presentation: 福岡
• [Presentation] formin 相同蛋白質FHOD3の筋細胞における生理的役割 (2009)
  • Author(s): 武谷立, 谷口賢一郎, 住本英樹
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Place of Presentation: 福岡
• [Presentation] Regulation of actin assembly and sarcomere organization in cardiomyocytes by the formin homology protein Fhod3 (2009)
  • Author(s): 谷口賢一郎, 武谷立, 末次志郎, 神尾明君, 成澤慈, 塩瀬明, 富永隆治, 住本英樹
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] The formin-homology protein Fhos/FHOD : Mechanisms of action and physiological functions. (2008)
  • Author(s): Ryu Takeya, Kenichiro Taniguchi, Shuh Narumiya, Hideki Sumimoto.
  • Organizer: 日本分子生物学会年会・日本生化学会大会・合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] The formin-homology protein Fhos/FHOD : Mechanisms of action and physiological functions. BMB2008 (2008)
  • Author(s): Ryu Takeya, Kenichiro Taniguchi, Shuh Narumiya, Hideki Sumimoto.
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸.シンポジウム
• [Presentation] The formin-homology protein Fhos/FHOD : Mechanisms of action and physiological functions. (2008)
  • Author(s): Ryu Takeya, Kenichiro Taniguchi, Shuh Narumiya, Hideki Sumimoto
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)シンポジウム「細胞骨格の創造から機能発現まで」
  • Place of Presentation: 神戸