Pathophysiological roles of the sphingosine-1-phosphate signaling system in vivo

• Project/Area Number: 20590288
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Ishikawa Prefectural Nursing University
• Principal Investigator: TAKUWA Noriko Ishikawa Prefectural Nursing University, 看護学部・健康科学講座, 教授 (70150290)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: スフィンゴシン-1-リン酸 / 遺伝子改変動物 / がん血管新生 / 心筋リモデリング / S1P2受容体 / G蛋白共役型受容体 / 低分子量G蛋白 / ノックアウトマウス / 骨髄移植実験 / マトリックスメタロプロテアーゼ / 心筋線維化 / スフィンゴシンキナーゼ / トランスジェニックマウス / 酸化ストレス / TGFbeta-Smad system / 生理活性脂質 / 腫瘍血管新生 / 細胞遊走 / 血管内皮

Research Abstract

Sphingosine-1-phosphate (S1P) is a plasma lipid mediator that is produced by sphingosine kinase 1 (SphK1) in erythrocytes to mediate diverse biological activities via 5 members of the G protein-coupled S1P receptor subtypes. We previously cloned S1PR2 as an orphan G protein-coupled receptor and identified S1P as the physiological ligand. In fact S1PR2 turned out to be the first G protein-coupled receptor that negatively regulates cell migration. In the present study we generated SphK1 transgenic mice and S1PR2 knockout mice to investigate in vivo roles for the S1P signaling system. We found that SphK1 transgenic mice show resistance against acute myocardial ischemia whereas they develop spontaneous cardiac remodeling and fibrosis through mechanisms involving S1PR3 and transactivation of TGFβ signaling pathway and reactive oxygen-dependent processes. We also found that deletion of S1PR2 resulted in enhancement of tumor angiogenesis and tumor growth. Studies in cultured lung endothelial cells and bone marrow transplantation experiments indicated that S1PR2 expressed on vascular endothelial cells and bone marrow-derived myeloid cells in concert inhibit tumor angiogenesis, resulting in suppression of tumor progression, providing molecular basis for anti-angiogenic cancer therapy with S1PR2-selective agonist.

Research Products

• [Journal Article] Tumor-suppressive sphingosine-1-phosphate receptor-2 counteracting tumor-promoting sphingosine-1-phosphate receptor-1 and sphingosine kinase 1-Jekyll Hidden behind Hyde. (2011)
  • Author(s): Takuwa N., Du W., Kaneko E., Okamoto Y., Yoshioka K., Takuwa Y.
  • Journal Title: Am J Cancer Res 1 Pages: 460-481
  • Peer Reviewed
• [Journal Article] Sphingosine-1-phosphate-specific G protein-coupled receptors as novel therapeutic targets for atherosclerosis. (2011)
  • Author(s): Okamoto Y., Wang F., Yoshioka K., Takuwa N., Takuwa Y.
  • Journal Title: Pharmaceuticals 4
  • URL: http://www.mdpi.com/1424-8247/4/1/117/pdf
  • Peer Reviewed
• [Journal Article] Tumor-suppressive sphingosine-1-phosphate receptor-2 counteracting tumor-promoting sphingosine-1-phosphate receptor-1 and sphingosine kinase 1----Jekyll Hidden behind Hyde. (2011)
  • Author(s): Takuwa N, Du W, Kaneko E, Okamoto Y, Yoshioka K, Takuwa Y.
  • Journal Title: Am J Cancer Res. Volume: 1 Pages: 460-481
  • Peer Reviewed
• [Journal Article] Sphingosine-1-phosphate-specific G protein-coupled receptorsas novel therapeutic targets for atherosclerosis. (2011)
  • Author(s): Okamoto Y, Wang F, Yoshioka K, Takuwa N, Takuwa Y.
  • Journal Title: Pharmaceuticals. Volume: 1 Pages: 117-137
  • Peer Reviewed
• [Journal Article] Roles of sphingosine-1-phosphate signaling in angiogenesis. (2010)
  • Author(s): Takuwa Y., Du W., Qi X., Okamoto Y., Takuwa N., Yoshioka K.
  • Journal Title: World J Biol Chem 1
  • Peer Reviewed
• [Journal Article] Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in Apoe-deficient mice. (2010)
  • Author(s): Wang F., Okamoto Y., Inoki I., Yoshioka K., Du W., Qi X., Takuwa N., Gonda K., Yamamoto Y., Ohkawa R., Nishiuchi T., Sugimoto N., Yatomi Y., Mitsumori K., Asano M., Kinoshita M., Takuwa Y.
  • Journal Title: J Clin Invest. 120 Pages: 3979-3995
  • NAID: 10029469757
  • Peer Reviewed
• [Journal Article] Sustained delivery of sphingosine-1-phosphate using poly (lactic-co-glycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice. (2010)
  • Author(s): Qi X, Okamoto Y, Murakawa T, Wang F, Oyama O, Ohkawa R, Yoshioka K, Du W, Sugimoto N, Yatomi Y, Takuwa N, Takuwa Y.
  • Journal Title: Eur J Pharmacol. 634 Pages: 121-131
  • Peer Reviewed
• [Journal Article] S1P_2, the G protein-coupled receptor for sphingosine-1-phosphate, negatively regulates tumor angiogenesis and tumor growth in vivo in mice. (2010)
  • Author(s): Du W, Takuwa N, Yoshioka K, Okamoto Y, Gonda K, Sugihara K, Fukamizu A, Asano M, Takuwa Y.
  • Journal Title: Cancer Res. 70 Pages: 772-781
  • Peer Reviewed
• [Journal Article] S1P_3-mediated cardiac fibrosis in sphingosine kinase 1 transgenic mice involves reactive oxygen species. (2010)
  • Author(s): Takuwa N, Ohkura S, Takashima S, Ohtani K, Okamoto Y, Tanaka T, Hirano K, Usui S, Wang F, Du W, Yoshioka K, Banno Y, Sasaki M, Ichi I, Okamura M, Sugimoto N, Mizugishi K, Nakanuma Y, Ishii I, Takamura M, Kaneko S, Kojo S, Satouchi K, Mitumori K, Chun J, Takuwa Y.
  • Journal Title: Cardiovasc Res. 85 Pages: 484-493
  • Peer Reviewed
• [Journal Article] Roles of sphingosine-1-phosphate signaling in angiogenesis. (2010)
  • Author(s): Takuwa Y, Du W, Qi X, Okamoto Y, Takuwa N, Yoshioka K.
  • Journal Title: World J Biol Chem. Volume: 1 Pages: 298-306
  • Peer Reviewed
• [Journal Article] Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in Apoe-deficient mice. (2010)
  • Author(s): Wang F, Okamoto Y, Inoki I, Yoshioka K, Du W, Qi X, Takuwa N, Gonda K, et al
  • Journal Title: J Clin Invest. Volume: 120 Pages: 3979-3995
  • NAID: 10029469757
  • Peer Reviewed
• [Journal Article] Sustained delivery of sphingosine-1-phosphate using poly(lactic-co-glycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice. (2010)
  • Author(s): Qi, X., Okamoto, Y., Murakawa, T., Wang, F., Oyama, O., Ohkawa, R., Yoshioka, K., Du, W., Sugimoto, N., Yatomi, Y., Takuwa.N., Takuwa, Y
  • Journal Title: Eur J Pharmacol. Volume: 634 Pages: 121-131
  • Peer Reviewed
• [Journal Article] S1P_2, the G protein-coupled receptor for sphingosine-1-phosphate, negatively regulates tumor angiogenesis andtumor growth in vivo in mice. (2010)
  • Author(s): Du, W, Takuwa.N., Yoshioka, K., Okamoto, Y., Gonda, K., Sugihara, K., Fukamizu, A., Asano, M., Takuwa, Y.
  • Journal Title: Cancer Research. Volume: 70 Pages: 772-81
  • Peer Reviewed
• [Journal Article] S1P_3-mediated cardiac fibrosis in sphingosine kinase 1 transgenic mice involves reactive oxygen species. (2010)
  • Author(s): Takuwa.N., Ohkura, S., Takashima, S., Ohtani, K., Okamoto, Y., et al.
  • Journal Title: Cardiovascular Research. Volume: 85 Pages: 484-93
  • Peer Reviewed
• [Journal Article] Sustained delivery of sphingosine-1-phosphate using poly(lactic-coglycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice (2010)
  • Author(s): Qi X, Okamoto Y, Murakawa T, Wang F, Oyama O, Ohkawa R, Yoshioka K, Du W, Sugimoto N, Yatomi Y, Takuwa N, Takuwa Y.
  • Journal Title: Eur J Pharmacol. (in press)
  • Peer Reviewed
• [Journal Article] S1P_2, the G protein-coupled receptor for sphingosine-1-phosphate, negatively regulates tumor angiogenesis and tumor growth in vivo in mice (2010)
  • Author(s): Du W, Takuwa N, Yoshioka K, Okamoto Y, Gonda K, Sugihara K, Fukamizu A, Asano M, Takuwa Y.
  • Journal Title: Cancer Res. 70(2) Pages: 772-81
  • Peer Reviewed
• [Journal Article] S1P_3-mediated cardiac fibrosis in sphingosine kinase 1 transgenic mice involves reactive oxygen species (2010)
  • Author(s): Takuwa N, Ohkura S, Takashima S, Ohtani K, Okamoto Y, Tanaka T, Hirano K, Usui S, Wang F, Du W, Yoshioka K, Banno Y, Sasaki M, Ichi I, Okamura M, Sugimoto N, Mizugishi K, Nakanuma Y, Ishii I, Takamura M, Kaneko S, Kojo S, Satouchi K, Mitumori K, Chun J, Takuwa Y.
  • Journal Title: Cardiovasc Res. 85(3) Pages: 484-93
  • Peer Reviewed
• [Journal Article] A case report of a renal mixed epithelial and stromal tumor in a heterozygous S1P_2 receptor deficient mouse. (2008)
  • Author(s): Takabatake M, Takuwa Y, Takuwa N, Yasuno H, Matsumoto S, Shibutani M, Mitsumori K.
  • Journal Title: J Vet Med Sci. 70 Pages: 483-485
  • Peer Reviewed
• [Journal Article] G_<(12)/(13)> and Gq mediate S1P_2-induced inhibition of Rac and migration in vascular smooth muscle in a manner dependent on Rho but not Rho kinase. (2008)
  • Author(s): Takashima S, Sugimoto N, Takuwa N, Okamoto Y, Yoshioka K, Takamura M, Takata S, Kaneko S, Takuwa Y.
  • Journal Title: Cardiovasc Res. 79 Pages: 689-697
  • Peer Reviewed
• [Journal Article] Sphingosine-1-phosphate signaling and biological activities in the cardiovascular system. (2008)
  • Author(s): Takuwa Y, Okamoto Y, Yoshioka K, Takuwa N.
  • Journal Title: Biochim Biophys Acta. 1781 Pages: 483-488
  • Peer Reviewed
• [Journal Article] The lysophospholipid mediator sphingosine-1-phosphate promotes angiogenesis in vivo in ischaemic hindlimbs of mice. (2008)
  • Author(s): Oyama O, Sugimoto N, Qi X, Takuwa N, Mizugishi K, Koizumi J, Takuwa Y.
  • Journal Title: Cardiovasc Res. 78 Pages: 301-307
  • Peer Reviewed
• [Journal Article] G12/13 and Gq mediate S1P2-induced inhibition of Rac and migration in vascular smooth muscle in, a manner dependent on Rhobut not Rho kinase. (2008)
  • Author(s): Takashima S, Sugimoto N, Takuwa N, Okamoto Y, Yoshioka K, Takamura M. Takata S, Kaneko S, Takuwa Y.
  • Journal Title: Cardiovasc Res. 79 Pages: 689-97
  • Peer Reviewed
• [Journal Article] A case report of a renal mixed epithelial and stromal tumor in a heterozygous S1P2 receptor deficient mouse. (2008)
  • Author(s): Takabatake M, Takuwa Y, Takuwa ti, Yasuno H. Matsumoto s. Shibutani M, Mitsumori K.
  • Journal Title: J Vet Med Sci. 70 Pages: 483-5
  • NAID: 110006688954
  • Peer Reviewed
• [Journal Article] The lysophospholipld mediator sphingoslne-1-phosphate promotes angiogenesis in vivo in ischaemic hindlimbs of mice. (2008)
  • Author(s): Oyama o. Sugimoto N, Qi x, Takuwa N. Mizugishi K, Koizumi J, Takuwa Y.
  • Journal Title: Cardiovasc Res. 78 Pages: 301-7
  • Peer Reviewed
• [Journal Article] Sphingosine-1-phosphate signaling and biological activities in the cardiovascular system. (2008)
  • Author(s): Takuwa Y. Okamoto Y, Yoshioka K, Takuwa N
  • Journal Title: Biochim Biophys Acta. 781 Pages: 483-8
  • Peer Reviewed
• [Presentation] Class II PI 3 kinase-C2α is essential for angiogenesis and barrier function. (2011)
  • Author(s): Yoshioka K, Yoshida K, Cui H, Aki S, Kuntal B, Takuwa N, Okamoto Y, Takuwa Y
  • Organizer: 第88回日本生理学会(誌上開催)
  • Place of Presentation: 横浜
• [Presentation] Deficiency of sphingosine-1-phosphate receptor-2 inhibits proinflammatory activities of macrophages and atherosclerosis in apoE-deficient mice. (2011)
  • Author(s): Okamoto Y, Wang F, Inoki I, Yoshioka K, Takuwa N, Takuwa Y
  • Organizer: 第88回日本生理学会(誌上開催)
  • Place of Presentation: 横浜
• [Presentation] Class II PI 3-kinase C2α is required for angiogenesis and barrier integrity. (2010)
  • Author(s): Yoshioka K, Takuwa N, Okamoto Y, Takuwa Y.
  • Organizer: 第18回 日本血管生物医学会学術集会/第8回日韓合同血管生物学シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-12-01
• [Presentation] The host cell sphingosine-1-phosphate receptor S1P2 negatively regulates tumor angiogenesis and tumor progression in vivo. (2010)
  • Author(s): 多久和典子, 杜娃, 吉岡和晃, 岡本安雄, 多久和陽
  • Organizer: 第69回日本癌学会学術総会ワークショップ
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] The host cell sphingosine-1-phosphate receptor SIP2 negatively regulates tumor angiogenesis and tumor progression in vivo. (2010)
  • Author(s): Takuwa N, Du W, Yoshioka K, Okamoto Y, Takuwa Y.
  • Organizer: 第69回日本癌学会学術総会 ワークショップ講演
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] Bimodal actions of the cardiac sphingosine-1-phosphate signaling revealed in sphingosine kinase 1 transgenic mice : S1P3-mediated, Rho-and active oxygen-dependent, chronic progression of spontaneous cardiac remodeling and cardioprotection against acutely imposed ischemia/reperfusion injury (2010)
  • Author(s): Takuwa N.
  • Organizer: The 27th Naito Conference "Membrane Dynamics and Llipid Biology[I]"
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-30
• [Presentation] S1P2受容体によるがん血管新生抑制とそのメカニズム (2010)
  • Author(s): Takuwa N, Du W, Yoshioka K, Okamoto Y, Takuwa Y.
  • Organizer: 第52回日本脂質生化学会シンポジウム1「リゾ脂質受容体研究の新展開」
  • Place of Presentation: 群馬
  • Year and Date: 2010-06-14
• [Presentation] Bimodal actions of the cardiac sphingosine-1-phosphate signaling revealed in sphingosine kinase 1 transgenic mice : S1P3-mediated, Rho-and active oxygen-dependent, chronic progression of spontaneous cardiac remodeling and cardioprotection against acutely imposed ischemia/reperfusion injury in sphingosine kinase 1 transgenic mice. (2010)
  • Author(s): Takuwa N, Ohkura S, Takashima S, Ohtani K, Usui S, .Okamoto Y, Wang F, Du W, Yoahioka K, Takuwa Y.
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-19
• [Presentation] The role of vascular Ca2+-PI3KC2 alpha-Rho axis in hypertension of spontaneously hypertensive rats. (2010)
  • Author(s): Okamoto Y, Seok Y-M, Azam MA, Sato A, Yoshioka K, Wang F, Hong C, Takuwa N, Takuwa Y.
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-19
• [Presentation] Class II PI 3-kinase C2α is required for angiogenesis and barrier integrity. (2010)
  • Author(s): 吉岡和晃, 多久和典子, 岡本安雄, 多久和陽
  • Organizer: 第18回日本血管生物医学会学術集会/第8回日韓合同血管生物学シンポジウム
  • Place of Presentation: 大阪
• [Presentation] Bimodal actions of the cardiac sphingosine-1-phosphate signaling revealed in sphingosine kinase 1 transgenic mice : S1P3-mediated, Rho-and active oxygen-dependent, chronic progression of spontaneous cardiac remodeling and cardioprotection against acutely imposed ischemia/reperfusion injury. (2010)
  • Author(s): 多久和典子.
  • Organizer: The 27th Naito Conference "Membrane Dynamics and Lipid Biology [I]"
  • Place of Presentation: 札幌
• [Presentation] S1P2受容体によるがん血管新生抑制とそのメカニズム (2010)
  • Author(s): 多久和典子, 杜娃, 吉岡和晃, 岡本安雄, 多久和陽
  • Organizer: 第52回日本脂質生化学会シンポジウム1「リゾ脂質受容体研究の新展開」
  • Place of Presentation: 伊香保(招待講演)
• [Presentation] Bimodal actions of the cardiac sphingosine-1-phosphate signaling revealed in sphingosine kinase 1 transgenic mice : S1P3-mediated, Rho-and active oxygen- dependent, chronic progression of spontaneous cardiac remodeling and cardioprotection against acutely imposed ischemia/reperfusion injury in sphingosine kinase 1 transgenic mice. (2010)
  • Author(s): Takuwa N, Ohkura S, Takashima S, Ohtani K, Usui S, Okamoto Y, Wang F, Du W, Yoahioka K, Takuwa Y
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] The role of vascular Ca^<2+>-PI3KC2alpha-Rho axis in hypertension of spontaneously hypertensive rats. (2010)
  • Author(s): Okamoto Y, Seok Y-M, Azam MA, Sato A, Yoshioka K, Wang F, Hong C, Takuwa N, Takuwa Y
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] Physiological role of class II PI3K-C2alpha for vascular formation : conditional knockout mouse study. (2010)
  • Author(s): Yoshioka K, Yoshida K, Aki S, Kuntal B, Du W, Qi, X, Wang F, Takuwa N, Okamoto Y, Takuwa Y
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] Cardiac remodeling and fibrosis in sphingosine kinase 1 transgenic mice through an S1P_3 receptor-mediated, reactive oxygen species-dependent process. (2010)
  • Author(s): Takuwa N., S.Ohkura, S.Takashima, K.Ohtani, Y.Okamoto, S.Usui, K.Yoshioka, M.Takamura, S.Kaneko, Y.Takuwa
  • Organizer: The 74^<th> Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 京都
• [Presentation] Cardiac remodeling and fibrosis in sphingosine kinase 1 transgenic mice through an S1P_3 receptor-mediated, reactive oxygen species-dependent process (2010)
  • Author(s): N.Takuwa, S.Ohkura, S.Takashima, K.Ohtani, Y.Okamoto, S.Usui, K.Yoshioka, M.Takamura, S.Kaneko, Y.Takuwa
  • Organizer: The 74^<th> Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 京都
• [Presentation] マウス後肢虚血モデルにおける徐放化S1P製剤を用いた血管新生療法 (2009)
  • Author(s): 戚〓, 岡本安雄, 村川智美, 尾山治, 王飛, 吉岡和晃, 杜娃, 杉本直俊, 多久和典子, 多久和陽
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 金沢
• [Presentation] 脂質メディエーターS1PによるS1P2受容体を介した腫瘍血管新生の抑制 (2009)
  • Author(s): 杜娃、多久和典子、吉岡和晃、岡本安雄, 多久和陽
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 金沢
• [Presentation] 心筋リモデリングにおけるスフィンゴシン-1-リン酸情報伝達系の病態生理学的役割-スフィンゴシンキナーゼ1トランスジェニックマウスを用いた検討- (2009)
  • Author(s): 多久和典子, 大倉誓一郎, 高島伸一郎, 大谷啓輔, 薄井荘一郎, 岡本安雄, 王飛, 杜娃, 吉岡和晃, 多久和陽
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 金沢
• [Presentation] マウス後肢虚血モデルにおける徐放化S1P製剤を用いた血管新生療法 (2009)
  • Author(s): 戚〓、岡本安雄、村川智美、王飛、尾山治、吉岡和晃、杜娃、多久和典子、多久和陽
  • Organizer: 第17回日本血管生物医学会
  • Place of Presentation: 東京
• [Presentation] The role of S1P_2, the angiogenesis inhibitory receptor for sphingosine-1-phosphate, in tumor growth and angiogenesis in vivo. (2009)
  • Author(s): Wa Du, 多久和典子, 吉岡和晃, 岡本安雄, Xun Qi, Fei Wang, Hong Cui, Kuntal Biswas, 杉原一司, 浅野雅秀, 多久和陽
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸(招待講演)
• [Presentation] Host cell S1P_2 receptor negatively regulates tumor growth and angiogenesis in vivo. (2009)
  • Author(s): 杜娃, 多久和典子, 吉岡和晃, 岡本安雄, 浅野雅秀, 多久和陽
  • Organizer: 4^<th> Interbnational Conference on Phospholopase A_2 and Lipid Mediators
  • Place of Presentation: 東京(招待講演)
• [Presentation] G_<(12)/(13)> and G_q coordinately mediate S1P_2-induced inhibition of Rac and migration in vascular smooth muscle in a Rho-dependent manner. (2009)
  • Author(s): Takashima S, Sugimoto N, Takuwa N, Okamoto Y, Yoshioka K, Takamura M, Kaneko S, Takuwa Y
  • Organizer: 第73回日本循環器学会大会
  • Place of Presentation: 大阪
• [Presentation] Host cell S1P_2 receptor negatively regulates tumor growth and angiogenesis in vivo (2009)
  • Author(s): Du W, Takuwa N, Yoshioka K, Okamoto Y, Asano M, Takuwa Y.
  • Organizer: 4^<th> Interbnational Conference on Phospholopase A_2 and Lipid Mediators
  • Place of Presentation: 東京
• [Presentation] The role of S1P_2, the angiogenesis inhibitory receptor for sphingosine-1-phosphate, in tumor growth and angiogenesis in vivo (2009)
  • Author(s): Wa Du, 多久和典子, 吉岡和晃, 岡本安雄, Xun Qi, Fei Wang, Hong Cui, Kuntal Biswas, 杉原一司, 浅野雅秀, 多久和陽
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] 脂質メディェーターS1PによるS1P2受容体を介した腫瘍血管新生の抑制 (2009)
  • Author(s): 杜娃, 多久和典子, 吉岡和晃, 岡本安雄, 多久和陽
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 金沢
• [Presentation] 心筋リモデリングにおけるスフィンゴシン-1-リン酸情報伝達系の病態生理学的役割 ―スフィンゴシンキナーゼ1トランスジェニックマウスを用いた検討― (2009)
  • Author(s): 多久和典子, 大倉誓一郎, 高島伸一郎, 大谷啓輔, 薄井荘一郎, 岡本安雄, 王飛, 杜娃, 吉岡和晃, 多久和陽
  • Organizer: 第56回中部日本生理学会
  • Place of Presentation: 金沢
• [Presentation] スフィンゴシン-1-リン酸(S1P)シグナリングと血管新生Sphingosine-1-phosphate signaling and angiogenesis(シンポジウム、リゾリン脂質の新しい機能Novel roles of lysophsopholipids) (2008)
  • Author(s): 多久和陽, 杜娃, 戚〓, 尾山治, 岡本安雄, 吉岡和晃, 多久和典子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸(招待講演)
• [Presentation] Sphingosine-1-phosphate receptor-2 (S1P2) negatively regulates tumor growth and angiogenesis in vivo. (2008)
  • Author(s): 杜娃, 多久和典子, 吉岡和晃, 岡本安雄, 浅野雅秀, 多久和陽
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 徐放化S1P製剤による虚血後血管新生の促進 (2008)
  • Author(s): 戚〓、村川智美、尾山治、杉本直俊、王飛、杜娃、吉岡和晃、岡本安雄、多久和典子、水岸貴代美、多久和陽
  • Organizer: 第50回日本脂質生化学会
  • Place of Presentation: 徳島
• [Presentation] 徐放化SIP製剤による虚血後血管新生の促進 (2008)
  • Author(s): 戚 〓, 村川智美, 尾山 治, 杉本直俊, 王 飛, 杜 娃, 吉岡和晃, 岡本安雄, 多久和典子, 水岸貴代美, 多久和 陽
  • Organizer: 第50回日本脂質生化学会
  • Place of Presentation: 徳島
• [Presentation] Sphingosine-1-phosphate receptor-2 (S1P2)negatively regulates tumor growth and angiogenesis in vivo. (2008)
  • Author(s): Du W, Takuwa N, YoshiokaK, Okamoto Y, Asano M, Takuwa Y.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Book] Lysophospholipid Receptors : Signaling and Biochemistry(Role of sphingosine-1-phosphate signaling in atherosclerosis.)(Chun J., Hla T., Spiegel S., Moolenaar W. eds., John Wiley & Sons, Hoboken) (2011)
  • Author(s): Takuwa Y., Wang F., Okamoto Y., Yoshioka K., Takuwa N.
  • Publisher: New Jersey, U.S.A(in press)