Identification of genes involved in the vascular network formation though neuro-vascular interactions
| Project/Area Number |
20590290
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
YONEKURA Hideto Kanazawa Medical University, 医学部, 教授 (80240373)
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| Co-Investigator(Kenkyū-buntansha) |
HATTA Toshihisa 金沢医科大学, 医学部, 教授 (20238025)
YOSHITAKE Yoshino 金沢医科大学, 医学部, 准教授 (00150764)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 遺伝子 / 血管内皮細胞 / 神経-血管相互作用 / ミクロRNA / 可溶型VEGF受容体 / レンチウイルスベクター / レンチイルスベクター |
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| Research Abstract |
We identified genes which were reproducibly up-regulated in HMVEC by the co-culture with dorsal root ganglion neurons and human microvascular endothelial cells (HMVEC). We found that hypoxia down-regulated soluble VEGF receptor-1 (soluble Flt-1, sFlt-1) expression in HMVEC and identified cis-elements that regulated sFlt-1 mRNA 3'-end processing. We found significantly reduced expression of esRAGE, a decoy receptor for amyloid β, in the hippocampal tissues from Alzheimer's disease brains by immunohistochemical analysis. We revealed that HMVEC expressed over 20 microRNAs including miR21. We injected lentiviral vectors into embryonic mouse skin and brain, and confirmed the expression of the introduced genes. We developed an assay method for esRAGE mRNA and an esRAGE transgenic mouse, and applied for patents for them.
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Report
(4 results)
Research Products
(67 results)
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[Journal Article] Septic shock is associated with receptor for advanced glycation endproducts (RAGE) ligation of LPS.2011
Author(s)
Yamamoto Y, Harashima A, Saito H, Tsuneyama K, Munesue S, Motoyoshi S, Han D, Watanabe T, Asano M, Takasawa S, Okamoto H, Shimura S, Karasawa T, Yonekura H, Yamamoto H.
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Journal Title
J.Immunol. 186
Pages: 3248-57
Related Report
Peer Reviewed
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[Journal Article] Identification of HITS, anewly identified TU3A/DDR1 family of protein (FAM107B), in cancer cells by heat shock stimulation.2010
Author(s)
Nakajima H, Ishigaki Y, Xia Q, Ikeda T, Yoshitake Y, Yonekura H, Nojima T, Tanaka T, Umehara H, Tomosugi N, Takada T, Shimasaki T, Nakaya N, Sato I, Koizumi K, Kawakami K, Minamoto T, Motoo Y.
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Journal Title
Int.J.Oncol. 37
Pages: 583-593
Related Report
Peer Reviewed
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[Journal Article] Paraquat toxicity induced by voltage-dependent anion channel 1 acting as an NADH-dependent oxidoreductase.2009
Author(s)
Shimada H, Hirai K-I, Simamura E, Hatta T, Iwakiri H, Mizuki K, Hatta T, Sawasaki T, Matsunaga S, Endo Y, Shimizu S.
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Journal Title
J.Biol.Chem. 284
Pages: 28642-28649
Related Report
Peer Reviewed
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[Journal Article] Solution structure of the variable-type domain of the receptor for advanced glycation end products : new insight into AGE-RAGE interaction.2008
Author(s)
Matsumoto S, Yoshida T, Murata H, Harada S, Fujita N, Nakamura S, Yamamoto Y, Watanabe T, Yonekura H, Yamamoto H, Ohkubo T, Kobayashi Y.
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Journal Title
Biochemistry 47
Pages: 12299-12311
Related Report
Peer Reviewed
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[Presentation] Enemies and friends within in the pathogenesis and prevention of diabetic vascular complications.2010
Author(s)
Yamamoto H, Watanabe T, Yamamoto Y, Yonekura H, Munesue S, Ohe K, Sugihara T, Saito H, Motoyoshi S, Han D, Win MTT, Abouzed TKMI, Ohara T.
Organizer
The 4th International HMGB1 Symposium SIGNALS OF TISSUE DAMAGE (Invited Lecture)
Place of Presentation
Helsinki, Finland
Related Report
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[Presentation] The role of AGE in atherosclerosis.2010
Author(s)
Yamamoto H, Watanabe T, Yamamoto Y, Yonekura H, Munesue S, Ohe K, Sugihara T, Saito H, Motoyoshi S, Han D, Win MTT, Abouzed TKMI, Ohara T.
Organizer
XXth World Congress of the International Society for Heart Research (Invited Lecture)
Place of Presentation
Kyoto, Japan
Related Report
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[Presentation] Enemies and Friends Within in the Pathogenesis and Prevention of Diabetic Vasculopathy.2009
Author(s)
Yamamoto H, Watanabe T, Yamamoto Y, Yonekura H, Munesue S, Ohe K, Sugihara T, Saito H, Motoyoshi S, Han D, Win MTT.
Organizer
2009 International Conference on Vascular Biology (Invited Lecture)
Place of Presentation
Zhunan, Taiwan
Related Report
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