Functional analyses of transcription factors of ATF-2 gene family members by using knockout mouse

• Project/Area Number: 20590294
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MAEKAWA Toshio The Institute of Physical and Chemical Research, 石井分子遺伝学研究室, 副主任研究員 (90201764)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ATF-2 / ATF-7 / ストレス / 転写制御 / エピジェネティクス / 肥満 / 行動異常 / セロトニン / ATF-2遺伝子ファミリー / ヘテロクロマチン / トリメチル化ヒストンH3K9 / ATF-7複合体 / DNA修復酵素 / CRE-BPa / HTR5B / p38阻害剤 / PPARγ2 / 肥満予防 / 脂肪分化 / p38 / 阻害剤

Research Abstract

By the analyses of knockout mice of the ATF-2 gene family members, I have analyzed the physiological functions of ATF-2 gene family members. I have found that ATF-2 contribute to the developments of central nervous systems and macrophages. ATF-2 and CRE-BPa also contribute to the differentiations to the adipose tissue. On the other hand, ATF-7 represses the transcription of serotonin receptor 5b in the dorsal raphe nucleus in the brain stem. By the depletion of ATF-7, serotonin concentration was reduced and mouse showed abnormal behaviors.

Research Products

• [Journal Article] ATF-7を介したストレスによるエピジェネティック変化 (2010)
  • Author(s): 前川利男、石井俊輔
  • Journal Title: 実験医学 エピジェネティクスと疾患 28(15) Pages: 169-175
• [Journal Article] The role of ATF-2 family transcription factor in adipocyte differentiation : Antiobesity effects of p38 inhibitors. (2010)
  • Author(s): Maekawa T, Jin W, Ishii S
  • Journal Title: Mol.Cell.Biol. 30 Pages: 613-625
  • Peer Reviewed
• [Journal Article] Socail isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene. (2010)
  • Author(s): Maekawa T, Kim S-J, Nakai D, Makino C, Takagi T, Orura H, Yamada K, Chatton B, Ishii S
  • Journal Title: EMBO J. 29 Pages: 184-195
  • Peer Reviewed
• [Journal Article] ATF-7を介したストレスによるエピジェネティック変化 (2010)
  • Author(s): 前川利男、石井俊輔
  • Journal Title: 実験医学 Volume: 28(15) Pages: 2489-2495
• [Journal Article] Social isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene (2010)
  • Author(s): Toshio Maekawa
  • Journal Title: The EMBO Journal 29 Pages: 184-195
  • Peer Reviewed
• [Journal Article] The role of ATF-2 family transcription factors in adipocyte differentiation : Antiobesity effects of p38 inhibitors (2010)
  • Author(s): Toshio Maekawa
  • Journal Title: Molecular and Cellular Biology 30 Pages: 613-625
  • Peer Reviewed
• [Journal Article] ATF-2 regulates lipopolysaccharide-induced transcription in macrophage cells. (2009)
  • Author(s): Hirose N, Maekawa T, Shinagawa T, Ishii S
  • Journal Title: Biochem Biophys Res Commun. 385 Pages: 72-77
  • Peer Reviewed
• [Journal Article] ATF-2 regulates lipopolysaccharide-induced transcription in macrophage cells (2009)
  • Author(s): Noriyuki Hirose
  • Journal Title: Biochem.Biophys.Res.Commun. 385 Pages: 72-77
  • Peer Reviewed
• [Journal Article] ATF-2 controls transcription of Maspin and GADD45a genes independently from p53 to suppress mammary tumors. (2008)
  • Author(s): Maekawa T, Sano Y, Shinagawa T, Sakuma T,Nomura S, Licht JD, Ishii S
  • Journal Title: Oncogene 27 Pages: 1045-1054
  • Peer Reviewed
• [Journal Article] Increased expression of tyrosine hydroxylase and anomalous neurites in catecholaminergic neurons of ATF-2 null mice. (2008)
  • Author(s): Kojima M, Suzuki T, Maekawa T, Ishii S, Sumi-Ichinose C, Nomura T, Ichinose H
  • Journal Title: J.Neurosci.Res. 86 Pages: 544-552
  • Peer Reviewed
• [Presentation] ATF-7依存的なヒストンH3メチル化を介したストレスによる転写制御 (2010)
  • Author(s): 前川利男、他
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] Stress-induced epigenetic regulation via ATF-2 family transcription factors (2010)
  • Author(s): S.Ishii, K.Seong, H.Shimizu, D.Li, T.Maekawa
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] Stress-induced epigenetic regulation via ATF-2 family transcription factors (2010)
  • Author(s): S.Ishii, K.Seong, H.Shimizu, D.Li, T.Maekawa
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市(シンポジウム)
  • Year and Date: 2010-12-08
• [Presentation] ATF-7依存的なヒストンH3メチル化を介したストレスによる転写制御 (2010)
  • Author(s): 前川利男, 他6名
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市(口頭発表)
  • Year and Date: 2010-12-08
• [Presentation] Social isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene (2009)
  • Author(s): T.Maekawa, et al.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-12
• [Presentation] Social isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene (2009)
  • Author(s): Toshio Maekawa
  • Organizer: 日本分子生物学会
  • Place of Presentation: (パシフィコ横浜)横浜市
  • Year and Date: 2009-12-12
• [Presentation] Social isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene (2009)
  • Author(s): T.Maekawa, S.Ishii
  • Organizer: The 24 Naito Conference on Nuclear Dynamics
  • Place of Presentation: Sapporo、Japan
  • Year and Date: 2009-06-25
• [Presentation] ATF-7依存的、ヒストンH3メチル化を介したストレスによる転写制御 (2008)
  • Author(s): 前川利男、他
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] Role of ATF-2 family of transcription factorsin adipocyte differentiation : anti-obesity effect of p38 inhibitor (2008)
  • Author(s): T.Maekawa, S.Ishii
  • Organizer: The 21 Naito Conference on Nuclear Dynamics and RNA I
  • Place of Presentation: Yamanashi、Japan
  • Year and Date: 2008-06-26
• [Presentation] ATF-7依存的、ヒストンH3メチル化を介したストレスによる転写制御 (2008)
  • Author(s): 前川利男
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートピア
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