| Project/Area Number |
20590317
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Nippon Medical School |
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Principal Investigator |
OKAMOTO Ken (2010) Nippon Medical School, 医学部, 准教授 (60267143)
西野 朋子 (2008-2009) Nippon Medical School, 医学部, 講師 (80075613)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Tomohiro 日本医科大学, 医学部, 助教 (20297930)
KUSANO Teruo 日本医科大学, 医学部, 助教 (30434129)
岡本 研 日本医科大学, 医学部, 准教授 (60267143)
西野 武士 日本医科大学, 大学院・医学研究科, 教授 (40094312)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 分子病態学 / スーパーオキシド / キサンチン酸化還元酵素 / キサンチン脱水素酵素 / キサンチン酸化酵素 / トランスジェニックマウス / キサンチチン酸化酵素 / キサンチチン脱水素酵素 / フラビン酵素 / モリブデン酵素 / 活性酸素 / ノックインマウス / 酸化酵素型-脱水素酵素型変換 / モリブデン酸素 |
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| Research Abstract |
We designed and constructed O2- hyperproduction XOR mutant knoched-in mouse which and dehydrogenase-type locked mutant knoched-in mouse. We propagated the starins. We purified mutant XOR from the knocked-in mutant mouse liver and confirmed the hyper production of superoxide anion. We are observing the phenotypes of the mutans and surveying pathological findings of organs.
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