Mechanisms of diseases by chromosomal translocation

• Project/Area Number: 20590326
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Research Institute, Shiga Medical Center
• Principal Investigator: KINOSHITA Kazuo Research Institute, Shiga Medical Center, 遺伝子研究部門, 専門研究員 (50293874)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 分子腫瘍学 / 染色体転座 / 染色体異常 / DNA組換え / 遺伝子間距離 / 染色体テリトリー / がん

Research Abstract

Chromosomal Translocations are one of major causes of various diseases including cancer, psychiatric disorders, and congenital anomalies. Understanding these diseases requires a technique to experimentally recapitulate diseases-specific chromosomal translocations. We improved previous methods to enable easier and quicker manipulation of mammalian chromosomes. Using this technique, we demonstrated recapitulations of two-types of cancer-causing chromosomal translocations and induction of intra-chromosomal duplication, deletion, and inversion. In addition, we applied it to measurement of distance between two genetic loci.

Research Products

• [Journal Article] Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. (2010)
  • Author(s): Uemura, M, Niwa, Y, Kakazu, N, Adachi, N, Kinoshita, K.
  • Journal Title: PLoS ONE vol.5.
  • Peer Reviewed
• [Journal Article] Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. (2010)
  • Author(s): Uemura, M, Niwa, Y, Kakazu, N, Adachi, N, Kinoshita, K
  • Journal Title: PLoS ONE 5
  • Peer Reviewed
• [Presentation] Cre組換え酵素による染色体間組換え頻度測定により明らかとなったテロメア近傍遺伝子のアクセシビリティー (2010)
  • Author(s): 植村宗弘, 丹羽陽子, 嘉数直樹、足立典隆、木下和生
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Cre組換え酵素による染色体間組換え頻度測定により明らかとなったテロメア近傍遺伝子のアクセシビリティー (2010)
  • Author(s): 植村宗弘, 丹羽陽子, 嘉数直樹、足立典隆、木下和生
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 部位特異的組換え酵素と蛍光タンパクを用いた染色体手術法の開発 (2010)
  • Author(s): 植村宗弘, 丹羽陽子, 木下和生
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2010-05-22
• [Presentation] 部位特異的組換え酵素と蛍光タンパクを用いた染色体手術法の開発 (2009)
  • Author(s): 植村宗弘、丹羽陽子、嘉数直樹、足立典隆、木下和生
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. (2009)
  • Author(s): 植村宗弘, 丹羽陽子, 嘉数直樹, 足立典隆, 木下和生.
  • Organizer: 21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 上海(中国)
  • Year and Date: 2009-08-09
• [Presentation] Cre-loxP組換え系と蛍光タンパクを用いた遺伝子間3次元的距離の測定 (2008)
  • Author(s): 植村宗弘、足立典隆、木下和生
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Cre-loxP 組換え系と蛍光タンパクを用いた遺伝子間3次元的距離の測定 (2008)
  • Author(s): 植村宗弘, 足立典隆, 木下和生
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Remarks] ホームページ等
  • URL: http://www.shigamed.jp/staff/kinoshita.html
• [Remarks]
  • URL: http://homepage.mac.com/kkinoshi/lab/
• [Remarks]
  • URL: http://homepage.mac.com/kkinoshi/lab/
• [Remarks]
  • URL: http://homepage.mac.com/kkinoshi/lab/
• [Remarks]
  • URL: http://homepage.mac.com/kkinoshi/lab/