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Identification of regulatory factors for genomic imprinting using siRNA library

Research Project

Project/Area Number 20590330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionSaga University

Principal Investigator

SOEJIMA Hidenobu  Saga University, 医学部, 教授 (30304885)

Co-Investigator(Kenkyū-buntansha) JOH Keiichiro  佐賀大学, 医学部, 准教授 (90124809)
HIGASHIMOTO Ken  佐賀大学, 医学部, 助教 (30346887)
KUGOH Hiroyuki  鳥取大学, 大学院・医学系研究科, 准教授 (40225131)
KOSEKI Haruhiko  理化学研究所, 免疫器官形成研究グループ・グループディレクター (40225446)
Research Collaborator YATSUKI Hitomi  佐賀大学, 医学部, 技術専門職員
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsエピジェネティクス / ゲノム刷り込み / 刷り込みドメイン / non-coding RNA / siRNAライブラリー / KIP2 / LIT1刷り込みドメイン
Research Abstract

The aim of this study is to clarify regulatory mechanisms of the imprinting. (1) To distinguish imprinting disrupted cells from normal cells by expression of marker genes, Igf2r was targeted with YFP cassette on the maternal allele in F1ES cell. We are trying to introduce Igf2r-2A-E2Crimsoncassette into the paternal allele. The cells will be screened with siRNA library to identify regulatory factors for the imprinting. (2) non-coding RNA LIT] would recruit HDAC(s) to the promoter region of KvLQTl to deacetylate histone H3 and to make heterochromatin, leading to repress the KvLQTl gene on the paternal chromosome. (3) At mouse Commdl/U2af1-rsl imprinted domain, that interference with paternal Commdl transcription by the oppositely directed U2afl-rs1 transcription seemed to be a mechanism for the maternal predominantly expression of Commdl gene.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (66 results)

All 2010 2009 2008 Other

All Journal Article (27 results) (of which Peer Reviewed: 14 results) Presentation (34 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Acute megakaryocytic leukemia (AMKL, FAB ; M7) with Beckwith-wiedemann syndrome.2010

    • Author(s)
      Yamamoto S, Soejima H, Isoyama K
    • Journal Title

      Pediatr Blood Cancer. 55(4)

      Pages: 733-735

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Organotypic culture of human bone marrow adipose tissue for analyzing its biological roles.2010

    • Author(s)
      Uchihashi K, Soejima H, Toda S
    • Journal Title

      Pathol Int 60(4)

      Pages: 259-267

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acute megakaryocytic leukemia (AMKL, FAB ; M7) with Beckwith-Wiedemann syndrome2010

    • Author(s)
      Yamamoto S
    • Journal Title

      Pediatr Blood Cancer

      Volume: 55 Pages: 733-735

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organotypic culture of human bone marrow adipose tissue for analyzing its biological roles2010

    • Author(s)
      Uchihashi K
    • Journal Title

      Pathol Int

      Volume: 60 Pages: 259-267

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antisense transcription occurs at the promoter of a mouse imprinted gene, Commdl, on the repressed paternal allele.2009

    • Author(s)
      Joh K, Yatsuki H, Higashimoto K, Mukai T, Soejima H
    • Journal Title

      J Biochem 146(6)

      Pages: 771-774

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] エピジェネティクス関連疾患と解析方法2009

    • Author(s)
      副島英伸
    • Journal Title

      臨床紡理 57(8)

      Pages: 769-778

    • NAID

      10026337428

    • Related Report
      2010 Final Research Report
  • [Journal Article] 小児秤特集小児疾患における臨床遺伝学の進歩2009

    • Author(s)
      東元健、副島英伸
    • Journal Title

      Beckwith-Wiedemann症候群 50(7)

      Pages: 1046-1052

    • Related Report
      2010 Final Research Report
  • [Journal Article] Wiedemann-Beckwith症候群2009

    • Author(s)
      副島英伸
    • Journal Title

      ビジュアル疾患解説目で見る遺伝病とターナー症候群株式会社メディアート(新川詔夫,緒方勤監修) No.5

    • Related Report
      2010 Final Research Report
  • [Journal Article] Antisense transcription occurs at the promoter of a mouse imprinted gene, Commd1, on the repressed paternal allele.2009

    • Author(s)
      Joh K, Yatsuki H(equal contribution)
    • Journal Title

      J Biochem 146

      Pages: 771-774

    • NAID

      10026305033

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] エピジェネティクス関連疾患と解析方法2009

    • Author(s)
      副島英伸
    • Journal Title

      臨床病理 57

      Pages: 769-778

    • NAID

      10026337428

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 特集小児疾患における臨床遺伝学の進歩Beckwith-Wiedemann症候群2009

    • Author(s)
      東元健
    • Journal Title

      小児科 50

      Pages: 1046-1052

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      副島英伸(新川詔夫, 吉浦孝一郎監訳)
    • Journal Title

      カラー図解基礎から疾患までわかる遺伝学 第2部ゲノミクス(メディカル・サイエンス・インターナショナル)

      Pages: 240-269

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      副島英伸(新川詔夫, 緒方勤監修)
    • Journal Title

      ビジュアル疾患解説 目で見る遺伝病とターナー症候群Wiedemann-Beckwith症候群(株式会社メディアート)

      Pages: 6-7

    • Related Report
      2009 Annual Research Report
  • [Journal Article] A new organotypic culture of adipose tissue fragments maintains viable mature adipocytes for a long term, together with development of immature adipocytes and mesenchymal stem cell-like cells.2008

    • Author(s)
      Sonoda E, Soejima H, Toda S
    • Journal Title

      Endocrinology 149(10)

      Pages: 4794-4798

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] MeCP2 knockdown reveals DNA methylation-independent gene repression of target genes in living cells and a bias in the cellular location of target gene products.2008

    • Author(s)
      Yakabe S, Soejima H, Higashimoto K, Joh K
    • Journal Title

      Genes Genet Syst 83(2)

      Pages: 199-208

    • NAID

      10024396087

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Duplication of paternal IGF2 or loss of maternal IGF2 imprinting occurs in half of Wilms tumors with various structural WT1 abnormalities.2008

    • Author(s)
      Haruta M, Soejima H, Kaneko Y
    • Journal Title

      Genes Chromosomes Cancer 47(8)

      Pages: 712-727

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] MeCP2-dependent repression of an imprinted miR-184 released by depolarization.2008

    • Author(s)
      Nomura T, Soejima H, Hatada I
    • Journal Title

      Hum Mol Genet 17(8)

      Pages: 1192-1199

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A BHD Germline Mutation Identified in an Asian Family with Birt-Hogg-Dube Syndrome.2008

    • Author(s)
      Misago N, Joh K, Soejima H
    • Journal Title

      Acta Dermato-Venereologica 88(4)

      Pages: 423-425

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] ゲノム刷り込みとBeckwith-Wiedemann症候群2008

    • Author(s)
      東元健, 副島英伸
    • Journal Title

      日本小児血液学会雑誌 22(3)

      Pages: 139-143

    • NAID

      10029309180

    • Related Report
      2010 Final Research Report
  • [Journal Article] ゲノムインプリンティング機構と疾患2008

    • Author(s)
      副島英伸
    • Journal Title

      臨床検査 52(6)

      Pages: 683-688

    • Related Report
      2010 Final Research Report
  • [Journal Article] 特集エピジェネティクス-最近の動向と疾患-ゲノムインプリンティング異常と疾患2008

    • Author(s)
      副島英伸
    • Journal Title

      最新医学 63(4)

      Pages: 83-90

    • Related Report
      2010 Final Research Report
  • [Journal Article] MeCP2 knockdown reveals DNA methylation-independent gene repression of target genes in living cells and a bias in the cellular location of target gene products.2008

    • Author(s)
      Yakabe S
    • Journal Title

      Genes Genet Syst 83

      Pages: 199-208

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Duplication of paternal IGF2 or loss of maternal IGF2 imprinting occurs in half of Wilms tumors with various struetural WT1 abnormalities.2008

    • Author(s)
      Haruta M
    • Journal Title

      Genes Chromosomes Cancer 47

      Pages: 712-727

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MeCP2-dependent repression of an imprinted miR-184 released by depolarization.2008

    • Author(s)
      Nomura T
    • Journal Title

      Hum Mol Genet 17

      Pages: 1192-1199

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ゲノム刷り込みとBeckwith-Wiedemann症候群2008

    • Author(s)
      東元 健
    • Journal Title

      日本小児血液学会雑誌 22

      Pages: 139-143

    • NAID

      10029309180

    • Related Report
      2008 Annual Research Report
  • [Journal Article] ゲノムインプリンティング機構と疾患2008

    • Author(s)
      副島英伸
    • Journal Title

      臨床検査 52

      Pages: 683-688

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 特集エピジェネティクス-最近の動向と疾患- ゲノムインプリンティング異常と疾患2008

    • Author(s)
      副島英伸
    • Journal Title

      最新医学 63

      Pages: 83-90

    • Related Report
      2008 Annual Research Report
  • [Presentation] Beckwith-Wiedemann症候群の臨床像とゲノム・エピゲノム解析2010

    • Author(s)
      副島英伸
    • Organizer
      第17回遺伝性疾患に関する出生前診断研究会
    • Place of Presentation
      那覇
    • Year and Date
      2010-11-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] インプリンティング異常と疾患2010

    • Author(s)
      副島英伸
    • Organizer
      ヒューマンサイエンス振興財団ポストゲノム医薬品開発WG勉強会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-29
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Beckwith-Wiedemann症候群のインプリンティング機構と患者解析2010

    • Author(s)
      副島英伸
    • Organizer
      九州大学母子総合研究リサーチコアカンファレンス
    • Place of Presentation
      福岡
    • Year and Date
      2010-03-19
    • Related Report
      2010 Final Research Report
  • [Presentation] 腫瘍細胞におけるエピジェネティックな遺伝子発現異常の分子機構2010

    • Author(s)
      副島英伸
    • Organizer
      第49回日本婦人科腫瘍学会学術集会
    • Place of Presentation
      佐賀
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] enome and epigenome analyses of an imprinting disease Beckwith-Wiedemann syndrome.2010

    • Author(s)
      副島英伸
    • Organizer
      The 4^<th> Asian Chromosome Colloquium.
    • Place of Presentation
      Beijing, China
    • Related Report
      2010 Final Research Report
  • [Presentation] ゲノム刷り込み疾患Beckwith-Wiedemann症候群およびPlacental mesenchymal dysplasiaのゲノム・エピゲノム解析2010

    • Author(s)
      副島英伸
    • Organizer
      第18回日本胎盤学会学術集会
    • Place of Presentation
      熊本
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Genome and epigenome analyses of an imprinting disease Beckwith-Wiedemann syndrome2010

    • Author(s)
      Soejima H
    • Organizer
      The 4^<th> Asian Chromosome Colloquium
    • Place of Presentation
      Beijing, China
    • Related Report
      2010 Annual Research Report
  • [Presentation] Role of Ash1l for transcriptional pause release2010

    • Author(s)
      Higashimoto K
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report
  • [Presentation] Clinical features and genome/epigenome analyses of Japanese patients with Beckwith-Wiedemann syndrome2010

    • Author(s)
      Yoshinaga H
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report
  • [Presentation] 本邦Beckwith-Wiedemann症候群の臨床像とゲノム・エピゲノム解析2010

    • Author(s)
      副島英伸
    • Organizer
      日本人類遺伝学会第55回大会
    • Place of Presentation
      大宮
    • Related Report
      2010 Annual Research Report
  • [Presentation] 巨大絨毛血管腫を合併した臨床的Beckwith-Wiedemann症候群の一例2010

    • Author(s)
      青木藍子
    • Organizer
      第18回日本胎盤学会学術集会
    • Place of Presentation
      熊本
    • Related Report
      2010 Annual Research Report
  • [Presentation] インプリンティング疾患Beckwith-Wiedemann症候群本邦例の臨床像とゲノム・エピゲノム解析2010

    • Author(s)
      副島英伸
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Related Report
      2010 Annual Research Report
  • [Presentation] 本邦Beckwith-Wiedemann症候群の臨床像とゲノム・エピゲノム解析2010

    • Author(s)
      吉永北斗
    • Organizer
      第4回日本エピジェネティクス研究会年会
    • Place of Presentation
      米子
    • Related Report
      2010 Annual Research Report
  • [Presentation] A role of histone methylation by Ash1L in the establishment of transcriptional memory.2010

    • Author(s)
      Higashimoto K
    • Organizer
      2010 Cold Spring Harbor Asia Conference "Epigenetics, Chromatin & Transcription"
    • Place of Presentation
      Suzhou, China
    • Related Report
      2010 Annual Research Report
  • [Presentation] Beckwith-Wiedemann症候群の遺伝子診断(遺伝カウンセリング)2009

    • Author(s)
      西久保敏也
    • Organizer
      第32回日本小児遺伝学会学術集会.
    • Place of Presentation
      奈良
    • Year and Date
      2009-04-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] エピジェネティクス関連疾患と解析方法2009

    • Author(s)
      副島英伸
    • Organizer
      第19回日本臨床化学会九州支部総会,第53回日本臨床検査医学会九州地方会
    • Place of Presentation
      福岡
    • Year and Date
      2009-02-14
    • Related Report
      2010 Final Research Report
  • [Presentation] Regulation of imprinted domains, mouse Murr1/U2af1-rs1, Human KIF2/LIT1 and IGF2/H19.2009

    • Author(s)
      副島英伸
    • Organizer
      18th Lake Shirakaba Conference
    • Place of Presentation
      Vedbaek, Denmark
    • Related Report
      2010 Final Research Report
  • [Presentation] Regulation of imprinted domains, mouse Murr1/U2af1-rs1, Human KIP2/LIT1 and IGF2/H19.2009

    • Author(s)
      Soejima H
    • Organizer
      18th Lake Shirakaba Conference
    • Place of Presentation
      Vedbaek, Denmark
    • Related Report
      2009 Annual Research Report
  • [Presentation] H19メチル化インプリントが患児と胎盤で異なるBeckkwith-Wiedemann症候群の1例2009

    • Author(s)
      東元健
    • Organizer
      第16回日本遺伝子診療学会大会
    • Place of Presentation
      札幌
    • Related Report
      2009 Annual Research Report
  • [Presentation] HISTONE METHYLATION BY MAMMALIAN ASH1L FACILITATES IN DUCTION OF HOX GENES IN RESPONSE TO RETINOIC ACID, THEREBY CONTRIBUTES TO ACQUISITION OF PROPER IDENTITIES OF CERVICAL VERTEBRAE.2009

    • Author(s)
      Higashimoto K
    • Organizer
      Cold Spring Harbor Meeting 2009 Mechanism of Eukaryotic Transcription
    • Place of Presentation
      NewYork
    • Related Report
      2009 Annual Research Report
  • [Presentation] ADH1BおよびALDH2における琉球諸島vs北部九州ヒト集団の比較遺伝学解析2009

    • Author(s)
      小金淵佳江
    • Organizer
      日本人類遺伝学会第54回大会
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] H19-DMRのメチル化インプリントは正常発生過程で変化しうる2009

    • Author(s)
      東元健
    • Organizer
      日本人類遺伝学会第54回大会
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] HISTONE METHYLATION BY MAMMALIAN ASH1L FACILITATES IN DUCTION OF HOX GENES IN RESPONSE TO RETINOIC ACID, TH EREBY CONTRIBUTES TO ACQUISITION OF PROPER IDENTITIES OF CERVICAL VERTEBRA.2009

    • Author(s)
      東元健
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] アルコール代謝関連遺伝子群の東アジア人特異的多型と連鎖不平衡解析2009

    • Author(s)
      小金淵佳江
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] H19-DMRのメチル化インプリントは正常発生過程で変化しうる2009

    • Author(s)
      東元健
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] 胎盤と臍帯血でDNAメチル化状態が異なったBeckwith-Wiedemann症候群の1例2008

    • Author(s)
      副島英伸
    • Organizer
      第15回遺伝性疾患に関する出生前診断研究会
    • Place of Presentation
      大分市
    • Year and Date
      2008-10-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Imprinted non-coding RNA LITl is required for paternal repression of genes within human KIP2/LIT1 imprinted domain.2008

    • Author(s)
      Soejima H
    • Organizer
      The 2008 EAUHGS Symposium & the 8th EAUHGS Annual Meeting
    • Place of Presentation
      札幌
    • Year and Date
      2008-07-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Regulation of imprinting in Beckwith-Wiedemann syndrome.2008

    • Author(s)
      副島英伸
    • Organizer
      Northeastern Asian Symposium on "Cancer Epigenetics".
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2010 Final Research Report
  • [Presentation] Different control mechanisms of two imprinted domains, KIP2/LIT1-and Murrl/U2af1-rs1.2008

    • Author(s)
      副島英伸
    • Organizer
      INTERNATIONAL SYMPOSIUM 「Decoding Epigenetic Code」
    • Place of Presentation
      Tokyo
    • Related Report
      2010 Final Research Report
  • [Presentation] ベックウィズ・ヴィーデマン症候群のインプリンティング機序2008

    • Author(s)
      副島英伸
    • Organizer
      大阪大学蛋白研セミナー「インプリンティング疾患発症機序の解明と治療に向けて」
    • Place of Presentation
      大阪大学医学部
    • Related Report
      2010 Final Research Report
  • [Presentation] Different control mechanisms of two imprinted domains, KIP2/LIT1 and Murrl/U2af1-rs1.2008

    • Author(s)
      Soejima H
    • Organizer
      INTERNATIONAL SYMPOSIUM「Decoding Epigenetic Code」
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒトKIP2/LIT1刷り込みドメインにおけるncRNA LIT1の及ぼす影響.2008

    • Author(s)
      八木ひとみ
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会BMB2008
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] ベックウィズ・ヴィーデマン症候群のインプリンティング機序2008

    • Author(s)
      副島英伸
    • Organizer
      大阪大学蛋白研セミナー「インプリンティング疾患発症機序の解明と治療に向けて」
    • Place of Presentation
      吹田市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Regulation of imprinting in Beckwith-Wiedemann syndrome2008

    • Author(s)
      Soejima H
    • Organizer
      Northeastern Asian Symposium on “Cancer Epigenetics"
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2008 Annual Research Report
  • [Book] 23章エピジェネティクスとヒト疾患(エピジェネティクス.D.アリス・T.ジェニュワイン・D.ラインバーグ共編, 堀越正美監訳)2010

    • Author(s)
      副島英伸, 城圭一郎, 中尾光善
    • Publisher
      培風館.東京
    • Related Report
      2010 Final Research Report
  • [Book] エピジェネティクス 23章エピジェネティクスとヒト疾患(D.アリス・T.ジェニュワイン・D.ラインバーグ共編,堀越正美監訳)2010

    • Author(s)
      副島英伸
    • Total Pages
      573
    • Publisher
      培風館
    • Related Report
      2010 Annual Research Report
  • [Book]2009

    • Author(s)
      副島英伸
    • Publisher
      メディカル・サイエンス・インターナショナル.東京(第2部ゲノミクス カラー図解基礎から疾憩までわかる遺伝学)(新川詔夫,吉浦孝一郎監訳)
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.biomol.med.saga-u.ac.jp/mbg/index.htm

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.biomol.med.saga-u.ac.jp/mbg/index.htm

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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