• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of model system including human ABCC11 transgene

Research Project

Project/Area Number 20590332
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

OHTA Tohru  北海道医療大学, 個体差健康科学研究所, 准教授 (10223835)

Co-Investigator(Kenkyū-buntansha) OIKAWA Tsuneyuki  北海道医療大学, 心理科学部, 教授 (80150241)
OSADA Kazumi  北海道医療大学, 歯学部, 准教授 (00382490)
YAMADA Takahiro  北海道大学, 大学病院, 助教 (20419948)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsABCC11 / 耳垢 / 乳がん / モデル細胞 / ABCC11遺伝子 / マウスモデル / 薬剤耐性遺伝子
Research Abstract

Human earwax is a secreted material from ceruminous apocrine glands, consisting of wet and dry types. Previous analysis demonstrated a SNP(c. 538G> A, rs17822931) in the ABCC11 gene is the earwax-type determinant. Body odor is sometimes one of the nervous matters for human. However, the chemical constitution for the odor was not yet clear. The human body odor is mainly discharged from apocrine glands of persons with G allele.
Curiously, rodent has genomic deletion of the full Abcc11 gene that might be evolutionally advantage to escape from enemy by means of no body odor. Thus, the mouse is a natural model system as dry type of ear wax.
Previous analysis demonstrated another polymorphism(Δ27) of the ABCC11.As the first project, we detected new two people withΔ27 from Japanese. One is a homozygote ofΔ27 and AA, the other is a heterozygote withΔ27 and GA. The second project was how association of breast cancer with the ABCC11 polymorphism. It was possible that the dry type could be associated with reduction of frequency in the estrogen receptor negative breast cancer. We have previously found a supplement which might reduce the body odor. However, scientific evaluation had not yet performed. As the third project, we analyzed the effect of the supplement against the human axillary odor for the people with ear wax G type. We detected significant difference between the people who took the supplement against the no supplement. As the last project, we have interested if the mouse has the wet type ABCC11, how the secreted material was increased or newly discharged. The mouse cell including the ABCC11 might also useful for a study of drug resistance. We constructed the transgene including the human genomic DNA of the ABCC11.However, the adequate expression was never observed in the mouse ES cells. Now we are ongoing to generate the model system for analysis the function of the ABCC11.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (27 results)

All 2012 2011 2010 2009

All Journal Article (10 results) (of which Peer Reviewed: 4 results) Presentation (15 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Down-regulation of ABCC11 protein(MRP8) in human breast cancer2011

    • Author(s)
      Sosonkina N, Nakashima M, Ohta T, Niikawa N, Starenki D.
    • Journal Title

      Exp Oncol

      Volume: 33(1) Pages: 42-6

    • Related Report
      2010 Final Research Report
  • [Journal Article] Spectrum of MLL2(ALR) mutations in 110 cases of Kabuki syndrome2011

    • Author(s)
      Hannibal MC, Buckingham KJ, Ng SB, Ming JE, Beck AE, McMillin MJ, Gildersleeve HI, Bigham AW, Tabor HK, Mefford HC, Cook J, Yoshiura K, Matsumoto T, Matsumoto N, Miyake N, Tonoki H, Naritomi K, Kaname T, Nagai T, Ohashi H, Kurosawa K, Hou JW, Ohta T, et al.
    • Journal Title

      Am J Med Genet A

      Volume: 155A(7) Pages: 1511-6

    • Related Report
      2010 Final Research Report
  • [Journal Article] Down-regulation of ABCC11 protein (MRP8) in human breast cancer2011

    • Author(s)
      Sosonkina N, et al.
    • Journal Title

      Exp Oncol

      Volume: 33(1) Pages: 42-46

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome2010

    • Author(s)
      Ng SB, Bigham AW, Buckingham KJ, Hannibal MC, McMillin MJ, Gildersleeve HI, Beck AE, Tabor HK, Cooper GM, Mefford HC, Lee C, Turner EH, Smith JD, Rieder MJ, Yoshiura K, Matsumoto N, Ohta T, et al.
    • Journal Title

      Nat. Genet

      Volume: 42(9) Pages: 790-793

    • Related Report
      2010 Final Research Report
  • [Journal Article] Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome2010

    • Author(s)
      Ng SB, et al.
    • Journal Title

      Nat.Genet.

      Volume: 42(9) Pages: 790-793

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] nese map of the earwax gene frequency : a nation-wide collaborative study by Super Science High School(SSH) Consortium2009

    • Author(s)
      S Sakai, K Imai, T Ogawa, H Iwaoka, M Ishii, S Komori, T Yoshida, H Jumonji, K Iizumi, H Ohshima, T Maeda, A Kanno, K Takahashi, H Kubota, Y Inoue, Y Takahashi, H Onoda, R Uchiyama, M Matsuda, T Akazawa, N Kawamura, T Odagiri, Y Watanabe, Y Matsumoto, S Shinoda, M Terada, M Matsuoka, C Ueno, E Ozaki, S Takaya, T Takeyama, T Hujita, K Kawakatsu, J Takemura, H Maekawa, T Doei, S Ihara, Y Sakaguchi, Y Hirota, A Shindo, H Araki, J Miura, T Morita, T Fujiwara, H Akiyama, S Itome, Y Tanaka, K Nakagawa, S Okamoto, S Yamamoto, T Aoyagi, T Noda, Inoue, I Hirota, K Tanaka, T Nagashima, I Koga, K Watanabe, H Kusadome, H Otsuka, T Takayama, H Miwa, A Hamakawa, K China, N Niikawa, T Ohta, et al.
    • Journal Title

      J Hum Genet

      Volume: 54 Pages: 499-503

    • Related Report
      2010 Final Research Report
  • [Journal Article] Developmentally dynamic changes of DNA methylation in the mouse Snurf/Snrpn gene2009

    • Author(s)
      Miyazaki K, Mapendano CK, Fuchigami Y, Kondo S, Ohta T, Kinoshita A, Tsukamoto K, Yoshiura K, Niikawa N, Kishino T.
    • Journal Title

      Gene

      Volume: 432 Pages: 97-101

    • Related Report
      2010 Final Research Report
  • [Journal Article] DNAメチル化と遺伝子発現制御のしくみを探る2009

    • Author(s)
      太田亨、新川詔夫
    • Journal Title

      Biophilia

      Volume: 5(3) Pages: 11-15

    • Related Report
      2010 Final Research Report
  • [Journal Article] Japanese map of the earwax gene frequency : a nationwide collaborative study by Super Science High School Consortium.2009

    • Author(s)
      Super Science High School Consortium
    • Journal Title

      J Hum Genet. 54

      Pages: 499-503

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmentally dynamic changes of DNA methylation the mouse Snurf/Snrpn gene.2009

    • Author(s)
      Miyazaki K, 他
    • Journal Title

      Gene. 432

      Pages: 97-101

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 日本人におけるヒト耳垢遺伝子ABCC11のΔ27アリルの新たな見解2010

    • Author(s)
      松田律史, 山田愛子, 小野佑輔, 堀佑輔, スタレンキディミトロ, ソソンキナナディア, 吉浦孝一郎, 太田亨, 新川詔夫
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド,神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] ホールエクソンキャプチャーによる歌舞伎メーキャップ症候群の解析2010

    • Author(s)
      要匡, 塚原正俊, 柳久美子, 藤森一浩, 喜久里育也, 照屋盛実, 今田有美, 鼠尾まい子, 矢野修一, 佐藤友紀, 三輪有希乃, 平野隆, 吉浦孝一郎, 太田亨, 新川詔夫, 成富研二
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド,神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] Frequency of 27-bp deletion mutation, another earwax determinant, in ABCC11 among the Japanese population2010

    • Author(s)
      A. Yamada, Y. Hori, Y. Ono, N. Matsuda, D. Starenki, N. Sosonkina, K. Yoshiura, T. Ohta, N. Niikawa
    • Organizer
      The American Society of Human Genetics, 59th Annual Meeting
    • Place of Presentation
      Washigton D. C., Baltimore
    • Related Report
      2010 Final Research Report
  • [Presentation] Discovery of a gene for Kabuki syndrome by exome sequencing and genotype-phenotype relationship in 110 cases2010

    • Author(s)
      M. J. Bamshad. M. C. Hannibal, K. J. Buckingham, A. E. Beck, S. B. Ng, M. McMillin, H. Gildersleeve, A. W. Bigham, H. K. Tabor, K. Yoshiura, T. Matsumot, N. Matsumoto, H. Tonoki, K. Naritomi, T. Kaname, T. Nagai, H. Ohashi, K. Kurosawa, J. Hou, T. Ohta, e
    • Organizer
      日本人類遺伝学会
    • Place of Presentation
      大宮ソニックシティー,大宮
    • Related Report
      2010 Final Research Report
  • [Presentation] 日本人におけるヒト耳垢遺伝子ABCC11のΔ27アリル頻度2010

    • Author(s)
      山田愛子, 堀佑輔, 小野佑輔, 松田律史, ストランキーディマ, ソソンキナナディア, 吉浦孝一郎, 新川詔夫, 太田亨
    • Organizer
      第55回日本人類遺伝学会
    • Place of Presentation
      大宮ソニックシティー,大宮
    • Related Report
      2010 Final Research Report
  • [Presentation] 歌舞伎メーキャップ症候群のエクソーム解析2010

    • Author(s)
      要匡, 塚原正俊, 柳久美子, 藤森一浩, 喜久里育也, 照屋盛実, 今田有美, 鼠尾まい子, 矢野修一, 佐藤友紀, 三輪有希乃, 平野隆, 吉浦孝一郎, 太田亨, 新川詔夫, 成富研二
    • Organizer
      第55回日本人類遺伝学会
    • Place of Presentation
      大宮ソニックシティー,大宮
    • Related Report
      2010 Final Research Report
  • [Presentation] ABCC11 expression and 538G/A poly-morphism in human breast cancer. Sosonkina Nadiya, Starenki Dmytro2010

    • Author(s)
      太田亨, 吉浦孝一郎, 新川詔夫
    • Organizer
      第55回日本人類遺伝学会
    • Place of Presentation
      大宮ソニックシティー,大宮
    • Related Report
      2010 Final Research Report
  • [Presentation] Frequency of 27-bp deletion mutation, another earwax determinant, in ABCC11 among the Japanese population2010

    • Author(s)
      A.Yamada, et al.
    • Organizer
      The American Society of Human Genetics, 59th
    • Place of Presentation
      Washington D.C., Baltimore, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] 日本人におけるヒト耳垢遺伝子ABCC11のΔ27アリルの新たな見解2010

    • Author(s)
      松田律史
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド,神戸(兵庫)
    • Related Report
      2010 Annual Research Report
  • [Presentation] ホールエクソンキャプチャーによる歌舞伎メーキャップ症候群の解析2010

    • Author(s)
      要匡
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド,神戸(兵庫)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Discovery of a gene for Kabuki syndrome by exome sequencing and genotype-phenotype relationship in 110 cases.2010

    • Author(s)
      M.J.Bamshad, et al.
    • Organizer
      第55回日本人類遺伝学会
    • Place of Presentation
      大宮ソニックシティー,大宮(埼玉)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 日本人におけるヒト耳垢遺伝子ABCC11のΔ27アリル頻度2010

    • Author(s)
      山田愛子
    • Organizer
      第55回日本人類遺伝学会
    • Place of Presentation
      大宮ソニックシティー,大宮(埼玉)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 歌舞伎メーキャップ症候群のエクソーム解析2010

    • Author(s)
      要匡
    • Organizer
      第55回日本人類遺伝学会
    • Place of Presentation
      大宮ソニックシティー,大宮(埼玉)
    • Related Report
      2010 Annual Research Report
  • [Presentation] ABCC11 expression and 538G/A polymorphism in human breast cancer.2010

    • Author(s)
      Sosonkina Nadiya
    • Organizer
      第55回日本人類遺伝学会
    • Place of Presentation
      大宮ソニックシティー,大宮(埼玉)
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA array-based copy number analysis in chorionic cillus samples(CVS) of spontaneous abortions with normal karyotypes2009

    • Author(s)
      Yamada T, Ohra T, et al.
    • Organizer
      The American Society of Human Genetics, 59th Annual Meeting
    • Place of Presentation
      Honolulu, Hawaii
    • Related Report
      2010 Final Research Report
  • [Book] 症候群ハンドブック2011

    • Author(s)
      井村裕夫(総編集)
    • Total Pages
      757
    • Publisher
      中山書店
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 体臭抑制剤2012

    • Inventor(s)
      長田和実、新川詔夫、太田亨
    • Industrial Property Rights Holder
      (特許出願人)学校法人東日本学園北海道医療大学
    • Industrial Property Number
      2012-016299
    • Filing Date
      2012-01-30
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi