| Project/Area Number |
20590339
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
YAMASHITA Yoriko Nagoya University, 大学院・医学系研究科, 講師 (90303643)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 骨髄 / びまん性大細胞型B細胞性リンパ腫 / 免疫グロブリン遺伝子 / ケモカイン / ケモカインリセプター / マイクロアレイ / XCR1 / B1細胞 |
|---|
| Research Abstract |
A total of 29 cases of diffuse large B-cell lymphoma initially manifesting in the bone marrow (BM-DLBCL) were analyzed for V_H gene sequence, and expression microarray of chemokines and chemokine receptors and immunohistochemical analysis were done. Seminested polymerase chain reaction (PCR) and sequencing analyses of 18 cases revealed that V_H3-7, V_H4-34, and V_H4-39 were frequently used, and the V_H gene usage in 14 of 18 V_H genes including VH3-48, VH3-23, VH3-21 were those associated with autoimmune diseases. Furthermore, cDNA microarray analysis specific for chemokine and chemokine receptors revealed that chemokine receptor XCR1 expression was significantly elevated in the BM-DLBCL cases (P<.05), which was confirmed by quantitative reverse transcriptase-PCR and immunohistochemical analysis. Expression of the chemokine receptor XCR1 and frequent usage of autoreactive V_H genes seem to be distinct characteristics of BM-DLBCL. Am J Clin Pathol, In press.
|
