Identification of a novel chimera gene not involving PAX family gene in rhabdomyosarcoma cell lines

• Project/Area Number: 20590365
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Shimane University
• Principal Investigator: KAKAZU Naoki 島根大学, 医学部, 准教授 (20264757)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 横紋筋肉腫 / 染色体転座 / 病型特異的転座 / キメラ遺伝子

Research Abstract

Rhabdomyosarcoma(RMS) is characterized by the specific chromosomal translocations involving 2q35 and 1p36 bands, which generate the PAX3-and PAX7-related chimera genes, respectively. We analyzed RMS cell lines by spectral karyotyping(SKY) and detected many chromosomal translocations, in which the PAX3 and PAX7 genes were not involved. We analyzed their breakpoint regions by FISH but could not identify novel chimera genes, which suggests that the translocations were secondarily caused by chromosomal instability due to the RMS development.

Research Products

• [Journal Article] Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. (2010)
  • Author(s): Uemura M, Niwa Y, Kakazu N, Adachi N, Kinoshita K.
  • Journal Title: PLoS One 5
  • Peer Reviewed
• [Journal Article] Restoration of p53 pathway by Nutlin-3 induces cell cycle arrest and apoptosis in human rhabdomyosarcoma cells (2009)
  • Author(s): Miyachi M, Kakazu N, Yagyu S, Katsumi Y, Tsubai-Shimizu S, Kikuchi K, Tsuchiya K, Iehara T, Hosoi H.
  • Journal Title: Clin Cancer Res Volume: 15 Pages: 4077-4084
  • Peer Reviewed
• [Journal Article] Restoration of p53 pathway by nutlin-3 induces cell cycle arrest and apoptosis in human rhabdomyosarcoma cells. (2009)
  • Author(s): Miyachi M, Kakazu N, et al.
  • Journal Title: Clin Cancer Res 15 Pages: 4077-4084
  • Peer Reviewed
• [Journal Article] The chromosomal constitution of postmitotic neurons, assessed by neuronal nuclear transfer into oocytes and in ES cell lines derived from them. (2009)
  • Author(s): Osada T, Kakazu N, et al.
  • Journal Title: Cytogenet Genome Res 125 Pages: 201-212
  • Peer Reviewed
• [Journal Article] A novel PAX3 rearrangement in embryonal rhabdomyosarcoma (2009)
  • Author(s): Hosoi H, Kakazu N, et al.
  • Journal Title: Cancer Genet Cytogenet 189 Pages: 98-104
  • Peer Reviewed