Comparative analysis of micro RNA expressions on between reactive lymphoid follicles and follicular lymphomas focusing to post-translational process.

• Project/Area Number: 20590366
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Okayama University
• Principal Investigator: KONDO Eisaku Okayama University, 腫瘍病理学部, 部長 (30252951)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分子病理 / 濾胞性リンパ腫 / microRNA / 胚中心 / トランスジェニックマウス / B細胞性リンパ腫 / リンパ腫 / マイクロダイセクション

Research Abstract

We planned the expression analysis of micro RNAs on between human follicular lymphomas (FLs) and their non-neoplastic counterpart, germinal centers (GCs) to elucidate the lymphomagenesis of FLs. From the results of miRNA profiling of human FLs in comparison with that of GCs, also of quantitative analysis by real-time PCR for sorted GC cells and FL cells, miR-146a and miR-193b revealed to be up-regulated in FLs than GCs, whereas miR-26a seemed to be down-regulated. Based on these results, we further generated transgenic mice overexpressing mouse miR-146a under regulation by immunoglobulin enhancer/promotor, and explore the phenotypic features of their B cells. Consequently, splenic GCs in miR-146a Tg mice seemed to be enlarged in response to stimulation by an exogenous antigen than those in wild type mice. The details are still under investigation now.

Research Products

• [Journal Article] Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins. (2011)
  • Author(s): Inoue T
  • Journal Title: Int.J.Oncol. Volume: 38(5) Pages: 1299-1306
  • Peer Reviewed
• [Journal Article] miR-155, a Modulator of FOXO3a Protein Expression, Is Underexpressed and Cannot Be Upregulated by Stimulation of HOZOT, a Line of Multifunctional Treg. (2011)
  • Author(s): Yamamoto M
  • Journal Title: PLoS One Volume: 3;6(2) Pages: 16841-16841
  • Peer Reviewed
• [Journal Article] NFATc 1 affects mouse splenic B cell function by controlling the calcineur in-NFAT signaling network. (2011)
  • Author(s): Bhattacharyya S
  • Journal Title: J.Exp.Med. Volume: 208(4) Pages: 823-839
  • Peer Reviewed
• [Journal Article] Micro RNA 130 family regulates the hypoxia response signal through the P-body protein DDX6 (2011)
  • Author(s): Saito, K.
  • Journal Title: Nuc.Acid.Res. Volume: 3;6(2)(Epub ahead of print) Pages: 12-12
  • Peer Reviewed
• [Journal Article] Intramuscular transplantation of engineered hepatictissueconstructs corrects acute and chronic liver failure in mice. (2010)
  • Author(s): Navarro-Alvarez N, Soto-Gutierrez A, Chen Y, Caballero-Corbalan J, Hassan W, Kobayashi S, Takei J, Kondo Y, Iwamuro M, Yamamoto K, Kondo E, Tanaka N,FoxIJ, Kobayashi N
  • Journal Title: Journal of Hepatology 52 Pages: 211-219
• [Journal Article] Germinal center-associated nuclear protein (GANP) is involved in mRNA export of Shugoshin-1 required for centromere cohesion and in sister-chromatid exchange. (2010)
  • Author(s): Okamoto N, Kuwahara K, Ohta K, Kitabatake M, Takagi K, Mizuta H, Kondo E Sakaguchi N.
  • Journal Title: Genes to Cells 15 Pages: 471-484
• [Journal Article] Isolation andpropagation of a human CD133-negative colon tumor-derived cell line withtumorigenic and angiogenic properties. (2010)
  • Author(s): *Navarro-Alvarez N, *Kondo E, *Soto-gutierrez A, Kawamoto H, Hassan W, YuasaT, Kubota Y, Seita M, Nakahara H, Hayashi T, Nishikawa Y, Hassan RARA,JavedSM, Noguchi H, Matsumoto S, Nakaji S, Tanaka N, Kobayashi N
  • Journal Title: Cell Transplantation 19 Pages: 865-877
• [Journal Article] Characteristics of CD133(+) human colon cancer SW620 cells. (2010)
  • Author(s): Kawamoto H, Yuasa T, Kubota Y, Seita M, Sasamoto H, Javed SM, Hayashi T,Nakahara H, Hassan R, Iwamuro M, Kondo E, Nakaji S, Tanaka N, Kobayashi N
  • Journal Title: CellTransplantation 19 Pages: 857-864
• [Journal Article] Targeted delivery of oligomannose-coated liposome to the omental micrometastasis by peritoneal macrophages from patients with gastric cancer. (2010)
  • Author(s): Matsui M, Shimizu, Y Ikehara Y, Kondo E, Kodera Y, Nakanishi H.
  • Journal Title: Cancer Sci 101 Pages: 1670-1677
  • NAID: 10026594802
• [Journal Article] Comparative analysis of endoderm formation efficiency between mouse EScellsand iPS cells. (2010)
  • Author(s): Iwamuro M, Komaki T, Kubota Y, Seita M, Kawamoto H, Yuasa T, Javed SM,Hassan RARA, Nakaji S, Nishikawa Y, Kondo E, Yamamoto K, Kobayashi N
  • Journal Title: Cell Transplantation 19 Pages: 831-839
• [Journal Article] Hepatic differentiation of mouse iPS cells in vitro. (2010)
  • Author(s): Iwamuro M, Komaki T, Kubota Y, Seita M, Kawamoto H, Yuasa T, Javed SM,Hassan RARA, Nakaji S, Nishikawa Y, Kondo E, Yamamoto K, Fox IJ, KobayashiN
  • Journal Title: CellTransplantation 19 Pages: 841-847
• [Journal Article] Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression. (2010)
  • Author(s): Nakaya T, Kuwahara K, Ohta K, Kitabatake M, Toda T, Takeda N, Tani T, Kondo E, Sakaguchi N.
  • Journal Title: J.Immunol 185 Pages: 5180-5187
• [Journal Article] Treatment of acute liver failure in mice by hepatocyte xenotransplantation. (2010)
  • Author(s): Yamamoto T, Navarro-Alvarez N, Soto-Gutierrez A, Yuasa T, Iwamuro M, Kubota Y, Seita M, Kawamoto H, Javed SM, Kondo E, Noguchi H, Kobayashi S, Nakaji S, Kobayashi N.
  • Journal Title: Cell Transplant 19 Pages: 799-806
• [Journal Article] Treatment of acute liver failure in mice by hepatocyte xenotrans plantation. (2010)
  • Author(s): Yamamoto T
  • Journal Title: Cell Transplant. Volume: 19(6) Pages: 799-806
  • Peer Reviewed
• [Journal Article] Comparative analysis of endoderm formation efficiency between mouse ES cells and iPS cells. (2010)
  • Author(s): Iwamuro M
  • Journal Title: Cell Transplant. Volume: 19(6) Pages: 831-839
  • Peer Reviewed
• [Journal Article] Hepatic differentiation of mouse iPS cells in vitro. (2010)
  • Author(s): Iwamuro M
  • Journal Title: Cell Transplant. Volume: 19(6) Pages: 841-847
  • Peer Reviewed
• [Journal Article] Characteristics of CD133(+) human colon cancer SW620 cells. (2010)
  • Author(s): Kawamoto Hobayashi N.
  • Journal Title: Cell Transplant. Volume: 19(6) Pages: 857-864
  • Peer Reviewed
• [Journal Article] Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression. (2010)
  • Author(s): Nakaya T
  • Journal Title: J.Immunol. Volume: 185(9) Pages: 5180-5187
  • Peer Reviewed
• [Journal Article] Targeted delivery of oligomannose-coated liposome to the omental micrometastasis by peritoneal macrophages from patients with gastric cancer. (2010)
  • Author(s): Matsui M
  • Journal Title: Cancer Sci. Volume: 101(7) Pages: 1670-1677
  • Peer Reviewed
• [Journal Article] Intramuscular transplantation of engineered hepatictissueconstructs corrects acute and chronic liver failure in mice. (2010)
  • Author(s): Navarro-Alvarez, N., Soto-Gutierrez, A., Chen, Y., Kondo, E., et al.
  • Journal Title: J. Hepatol. 52 Pages: 211-219
  • Peer Reviewed
• [Journal Article] Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice. (2009)
  • Author(s): Nakahara H, Misawa H, Hayashi T, Kondo E, Yuasa T, Kubota Y, Seita M, Kawamoto H, Hassan WA, Hassan RA, Javed SM, Tanaka M, Endo H, Noguchi H, Matsumoto S, Takata K, Tashiro Y, Nakaji S, Ozaki T, Kobayashi N.
  • Journal Title: Transplantation. 88(3) Pages: 346-353
• [Journal Article] Duodenal and nodal follicular lymphomas are distinct: the former lacks activation-induced cytidine deaminase and follicular dendritic cells despite ongoing somatic hypermutations. (2009)
  • Author(s): Takata K, Sato Y, Nakamura N, Kikuti YY, Ichimura K, Tanaka T, Morito T, Tamura M, Oka T, Kondo E, Okada H, Tari A, Yoshino T.
  • Journal Title: Mod Pathol 22 Pages: 940-949
• [Journal Article] Frequent downregulation or loss of CD79a expression in plasma cell myelomas : potential clue for diagnosis. (2009)
  • Author(s): Tanaka T, Ichimura K, Sato Y, Takata K, Morito T, Tamura M, Kondo E, Ohara N, Yanai H, Sakai M, Takahashi S, Yoshino T.
  • Journal Title: Pathol Int 59 Pages: 804-808
  • NAID: 10027382208
• [Journal Article] Duodenal and nodal follicular lymphomas are distinct : the former lacks activation-induced cytidine deaminase and follicular dendritic cells despite ongoing somatic hypermutations. (2009)
  • Author(s): Takata, K., Sato, Y., Nakamura, N., Kondo, E., etal.
  • Journal Title: Mod Pathol 22 Pages: 940-949
  • Peer Reviewed
• [Journal Article] Frequent downregulation or loss of CD79a expression in plasma cell myelomas : potential clue for diagnosis. (2009)
  • Author(s): Tanaka, T., Ichimura, K., Sato, Y., Kondo, E., et, al.
  • Journal Title: Pathol Int. 59 Pages: 804-808
  • NAID: 10027382208
  • Peer Reviewed
• [Journal Article] Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice. (2009)
  • Author(s): Nakahara, H., Misawa, H., Hayashi, T., Kondo, E., et, al.
  • Journal Title: Transplantation 88 Pages: 346-353
  • Peer Reviewed
• [Journal Article] Duodenal follicular lymphomas share common characteristics with mucosa-associated lymphoid tissue lymphomas. (2008)
  • Author(s): Sato, Y., Ichimura, K., Tanaka, T., Takata, K., Morito, T., Sato, H., Sato, Y., Kondo, E., Yanai, H., Ohara, N., Oka,. T, Yoshino, T.
  • Journal Title: J.Clin.Pathol. 61 Pages: 377-381
• [Journal Article] Epigenetic Changes and Suppression of the Nuclear Factor of Activated T Cell 1 (NFATC1) Promoter in Human Lymphomas with Defects in Immunoreceptor Signaling. (2008)
  • Author(s): Akimzhanov, A., Krenacs, L., Schlegel, T., Klein-Hessling, S., Bagdi, E., Stelkovics, E., Kondo, E., Chuvpilo, S., Wilke, P., Avots ,A., Gattenlohner, S., Muller-Hermelink, H-K., Palmetshofer, A., Serfling, E.
  • Journal Title: Am.J.Pathol. 172 Pages: 215-224
• [Journal Article] Potent synergy of dual antitumor peptides for growth suppression of human glioblastoma cell lines. (2008)
  • Author(s): Kondo, E., Tanaka, T., Miyake, T., Ichikawa, T., Hirai, M., Adachi, M., Yoshikawa, K., Ichimura, K., Ohara, N., Moriwaki, A., Date, I., Ueda, R., Yoshino, T.
  • Journal Title: Mol.Cancer Ther. 7 Pages: 1461-1471
• [Journal Article] Induction of lung adenocarcinoma in transgenic mice expressing activated EGFR driven by the SP-C promoter. (2008)
  • Author(s): Ohashi, K., Rai, K., Fujiwara, Y., Osawa, M., Hirano, S., Takata, K., Kondo, E., Yoshino, T, Takata, M., Tanimoto, M.
  • Journal Title: Cancer Sci. 99(9) Pages: 1747-53
  • NAID: 10024010760
• [Journal Article] Ocular adnexal IgG4-related disease has uniform clinicopathology. (2008)
  • Author(s): Sato, Y., Ohshima, K., Ichimura, K., Sato, M., Yamadori, I., Tanaka, T., Takata, K., Morito,Y, Kondo, E., Yoshino, T.
  • Journal Title: Pathol.Int. 58 Pages: 465-470
  • NAID: 10027377286
• [Presentation] Molecular targeting and imaging of gastric cancer metastasis (2011)
  • Author(s): Hayao Nakanishi
  • Organizer: BITs 3rd International Congress of Antibodies-2011(ICA-2011)
  • Place of Presentation: China National Convention Center(China)
  • Year and Date: 2011-03-24
• [Presentation] HER2過剰発現胃癌細胞に対する分子標的治療の検討 (2011)
  • Author(s): 中西速夫
  • Organizer: 第83回日本胃癌学会総会
  • Place of Presentation: 青森屋(青森)
  • Year and Date: 2011-03-05
• [Presentation] 胃癌腹膜転移に対するPTX新規感受性予測因子の網羅的探索 (2011)
  • Author(s): 村上弘城
  • Organizer: 第83回日本胃癌学会総会
  • Place of Presentation: 青森屋(青森)
  • Year and Date: 2011-03-04
• [Presentation] Development of novel cancer cell-selective cell-penetrating peptides for the advanced peptide-based drug delivery system. (2010)
  • Author(s): Eisaku Kondo
  • Organizer: 22nd EORTC-NCI-AACR Symposium
  • Place of Presentation: Berlin Estrel convention center(Germany)
  • Year and Date: 2010-11-18
• [Presentation] がん細胞選択的高透過性ペプチドを応用した抗腫瘍ペプチドデリバリー系の開発 (2010)
  • Author(s): 近藤英作
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-09-24
• [Presentation] 低分化型大腸癌におけるHER3の発現低下の機構とその意義 (2010)
  • Author(s): 田中晴就
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-09-24
• [Presentation] HER2遺伝子増幅胃がんに対するTrastuzumabとLapatinibの抗腫瘍効果の差異とその分子機構の解析 (2010)
  • Author(s): 中西速夫
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-09-23
• [Presentation] アデノウイルス受容体(CAR;CXADR)の固形癌細胞の増殖制御における役割の解析 (2010)
  • Author(s): 小屋恵理子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-09-23
• [Presentation] 頭頸部扁平上皮癌細胞株におけるEMT(上皮間葉移行)とEGFR標的薬の感受性の検討 (2010)
  • Author(s): 欄真一郎
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-09-22
• [Presentation] 分子標的ツールとしてのがん選択的細胞膜浸潤性を発揮する新規高透過能ペプチドの開発研究 (2010)
  • Author(s): 近藤英作
  • Organizer: 第14回日本がん分子標的治療学会学術集会(ワークショップ)
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2010-07-07
• [Presentation] がん幹細胞様形質を示す口腔扁平上皮癌細胞株のGefitinib感受性とその機構 (2010)
  • Author(s): 中西速夫
  • Organizer: 第19回日本がん転移学会
  • Place of Presentation: 金沢市文化ホール(石川県)
  • Year and Date: 2010-06-16
• [Presentation] がん幹細胞様形質を示す大腸がん低分化型腺癌細胞株のgefitinib高感受性 (2010)
  • Author(s): 中西速夫
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-04-28
• [Presentation] ヒト各種悪性腫瘍細胞におけるCXADR分子の発現の特徴とその生物学的役割についての解析 (2010)
  • Author(s): 松井誠
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-04-28
• [Presentation] (2010)
  • Organizer: 第99回日本病理学会総会
• [Presentation] (2010)
  • Organizer: 第99回日本病理学会総会
• [Presentation] (2010)
  • Organizer: 第19回日本がん転移学会総会
• [Presentation] (2010)
  • Organizer: 第14回日本がん分子標的治療学会
• [Presentation] (2010)
  • Organizer: 第69回日本癌学会学術総会
• [Presentation] (2010)
  • Organizer: 第69回日本癌学会学術総会
• [Presentation] (2010)
  • Organizer: 第69回日本癌学会学術総会
• [Presentation] (2010)
  • Organizer: 第69回日本癌学会学術総会
• [Presentation] (2010)
  • Organizer: 第69回日本癌学会学術総会
• [Presentation] (2010)
  • Organizer: 第83回日本胃癌学会総会
• [Presentation] (2010)
  • Organizer: 第83回日本胃癌学会総会
• [Presentation] (2010)
  • Organizer: 22^<nd> EORTC-NCI-AACR Sympojium
• [Presentation] Isolation and utility of Cell Penetrative Peptides selectively incorporated into human cancer cells. がん細胞選択的透過性ペプチドの開発とその応用 (2009)
  • Author(s): 近藤英作
  • Organizer: 日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] 胚中心におけるRelファミリー転写因子の発現と役割 (2009)
  • Author(s): 近藤英作
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-02
• [Presentation] (2009)
  • Organizer: 第98回日本病理学会総会
• [Presentation] (2009)
  • Organizer: 第98回日本病理学会総会
• [Presentation] (2009)
  • Organizer: 第13回日本がん分子標的治療学会
• [Presentation] (2009)
  • Organizer: 第68回日本癌学会学術総会
• [Presentation] (2009)
  • Organizer: The 2^<nd> Annual Protein and Peptide Conference (PepCon-2009)
• [Presentation] (2009)
  • Organizer: CTS-JSOPMB Joint Conference Symposium
• [Presentation] (2008)
  • Organizer: 第97回日本病理学会総会
• [Presentation] (2008)
  • Organizer: 第97回日本病理学会総会
• [Presentation] (2008)
  • Organizer: 第12回日本がん分子標的治療学会
• [Presentation] (2008)
  • Organizer: 第67回日本癌学会学術総会
• [Presentation] (2008)
  • Organizer: 第3回産学官連携新産業創出研究会
• [Presentation] (2008)
  • Organizer: Ehrlich II-2nd world Conference on Magic Bullets
• [Presentation] (2008)
  • Organizer: 20^<th> EORTC-NCI-AACR Sympojium
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] 癌細胞選択的膜透過性ペプチドおよびその利用 (2011)
  • Inventor(s): 近藤英作, 松下正之
  • Industrial Property Rights Holder: 愛知県, 三菱化学
  • Industrial Property Number: 2010-088186
  • Filing Date: 2011-04-05
  • Overseas
• [Patent(Industrial Property Rights)] 癌細胞選択的膜透過性ペプチドおよびその利用 (2010)
  • Inventor(s): 近藤英作, 松下正之
  • Industrial Property Rights Holder: 愛知県, 三菱化学
  • Industrial Property Number: 2010-088186
  • Filing Date: 2010-04-06