Molecular mechanisms of invasion and metastasis in bone and soft-tissue sarcomas.

• Project/Area Number: 20590375
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Juntendo University
• Principal Investigator: SAITO Tsuyoshi Juntendo University, 医学部, 助教 (80439736)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 悪性骨軟部腫瘍 / 転移・浸潤 / 分子治療標的 / 肉腫 / 治療抵抗性 / 分子標的治療 / 骨軟部腫瘍 / 浸潤・転移 / キメラ融合遺伝子 / RNAi / リセプター型チロシンキナーゼ

Research Abstract

We identified EWS-WT1(KTS+) fusion gene as a novel therapeutic target in desmoplastic small round cell tumor. Furthermore, exogenous expression of EWS-WT1(KTS+) in HeLa cells resulted in the acquisition of epithelial characteristics as evidenced by E-cadherin and β-catenin expression. Identification of p53 mutation in giant-cell tumor of bone might be a molecular pathological marker for malignant transformation of this tumor.

Research Products

• [Journal Article] Overexpression of HER2 signaling to WAVE2-Arp2/3 complex activates MMP-independent migration in breast cancer. (2011)
  • Author(s): Yokotsuka M, Iwaya K, Saito T, Pandiella A, Tsuboi R, Kohno N, Matsubara O, Mujkai K
  • Journal Title: Breast cancer Res Treat. 126 Pages: 311-318
• [Journal Article] A case of secondary malignant giant-cell tumor of bone with p53 mutation after long-time follow-up. (2011)
  • Author(s): Saito T, Mitomi H, Izumi H, et al
  • Journal Title: Human Pathol. 42 Pages: 727-733
• [Journal Article] A case of secondary malignant giant-cell tumor of bone with p53 mutation after long-time follow-up. (2011)
  • Author(s): Saito T, et al.
  • Journal Title: Human Pathology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Oncocytic mucoepidermoid carcinoma of the paroyid gland with CRTC1-MAML2 fusion transcript : Report of a case with review of literatures. (2011)
  • Author(s): Fujimaki M, Fukumura Y, Saito T, et al.
  • Journal Title: Human Pathology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Role of p16INK4a in progression of gastrointestinal stromal tumor of the stomach : alterations of members of p16INK4a network. (2011)
  • Author(s): Mitomi H, Fukui N, Kishimoto I, Tanabe S, Kikuchi S, Saito T, at al.
  • Journal Title: Human Pathology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Cluster analysis of claudin-1, and -4, E-cadherin and β-catenin expression in colorectal cancers. (2011)
  • Author(s): Matsuoka T, Mitomi H, Fukui N, Kanazawa H, Saito T, at al.
  • Journal Title: J Surg Oncol Volume: (In press)
  • Peer Reviewed
• [Journal Article] Abnormalities of Wnt/b-catenin signaling pathway induce tumor progression in desmoid tumors : correlation between β-catenin widespread nuclear expression and VEGF overexpression. (2011)
  • Author(s): Matono H, Tamiya S, Yokoyama R, Saito T, at al.
  • Journal Title: Histopathology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Subtype-specific genomic alterations define new targets for soft-tissue sarcoma therapy. (2010)
  • Author(s): Barretina J, Taylor BS, Saito T, et al
  • Journal Title: Nat Genet 42 Pages: 715-721
• [Journal Article] Subtype-specific genomic alterations define new targets for soft-tissue sarcoma therapy. (2010)
  • Author(s): Barretina J, Taylor BS, Saito T, at al.
  • Journal Title: Nat Genet Volume: 42(8) Pages: 715-721
  • Peer Reviewed
• [Journal Article] Aberrant p16((INK4a))methylation is a frequent event in colorectal cancers : prognostic value and relation to mRNA expression and immunoreactivity (2010)
  • Author(s): Mitomi H, et al.
  • Journal Title: Journal of Cancer Research and Clinical Oncology 138(2) Pages: 323-331
  • Peer Reviewed
• [Journal Article] Correlation between β-catenin widespread nuclear expression and matrix metalloproteinase-7 overexpression in sporadic desmoids tumors. (2008)
  • Author(s): Matono H, Saito T, et al.
  • Journal Title: Human Pathology 39(12) Pages: 1802-1808
  • Peer Reviewed
• [Journal Article] Oncocytic mucoepidermoid carcinoma of the paroyid gland with CRTC1-MAML2 fusion transcript : Report of a case with review of literatures.
  • Author(s): Fujimaki M, Fukumura Y, Saito T, et al
  • Journal Title: Human Pathol (in press)
• [Journal Article] Role of p16INK4a in progression of gastrointestinal stromal tumor of the stomach : alterations of members of p16INK4a network.
  • Author(s): Mitomi H, Fukui N, Kishimoto I, Tanabe S, Kikuchi S, Saito T, et al
  • Journal Title: Human Pathol (in press)
• [Journal Article] Cluster analysis of claudin-1, and -4, E-cadherin and β-catenin expression incolorectal cancers.
  • Author(s): Matsuoka T, Mitomi H, Fukui N, Kanazawa H, Saito T, et al
  • Journal Title: J Surg Oncol (in press)
• [Journal Article] Abnormalities of Wnt/β-catenin signaling pathway induce tumor progression in desmoid tumors : correlation between β-catenin widespread nuclear expression and VEGF overexpression.
  • Author(s): Matono H, Tamiya S, Yokoyama R, Saito T, et al
  • Journal Title: Histopathology (in press)
• [Presentation] デスモイド腫瘍におけるβ-catenin遺伝子異常のタイプと標的遺伝子の活性化能および細胞増殖能の比較検討 (2010)
  • Author(s): 齋藤剛, ら
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 初発から23年、再発治療後16年経過した後に悪性転化した骨巨細胞腫の1例 (2010)
  • Author(s): 齋藤剛, ら
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 胃間質細胞(GIST)におけるp16INK4a(p16)/Retinoblastoma(Rb)経路の破綻とその臨床病理学的意義 (2010)
  • Author(s): 三富弘之、松岡隆、齋藤剛, ら
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] Claudin-1,Claudin-4,E-cadherin,β-cateninの発現低下は大腸癌における予後不良因子である (2010)
  • Author(s): 松岡隆、三富弘之、齋藤剛, ら
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] Claudin-1, Claudin-4, E-cadherin, β-cateninの発現低下は大腸癌における予後不良因子である (2010)
  • Author(s): 松岡隆、三富弘之、齋藤剛, ら
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] Desmoplastic small round cell tumor におけるFGFR4高発現の意義とFGFR4シグナルの治療標的としての可能性 (2009)
  • Author(s): 齋藤剛, ら
  • Organizer: 日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-03
• [Presentation] Desmoplastic small round cell tumorにおけるFGFR4高発現の意義とFGFR4シグナルの治療標的としての可能性 (2009)
  • Author(s): 齋藤剛, ら
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 大阪
• [Presentation] 滑膜肉腫における上皮間葉移行および間葉上皮移行-SYT-SSX融合遺伝子と細胞間接着蛋白の関与- (2008)
  • Author(s): 齋藤 剛
  • Organizer: 第54回日本病理学会秋季特別総会
  • Place of Presentation: 松山
  • Year and Date: 2008-11-20
• [Presentation] Infarcted thymomaの1例 (2008)
  • Author(s): 野村将春、松林純、長尾俊孝、齋藤剛、永井毅、井上理恵、高橋礼典、泉美貴、草間博、向井清
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] SYT-SSX1 variantの上皮分化誘導能について (2008)
  • Author(s): 齋藤剛, ら
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] SYT-SSX1 variantの上皮分化誘導能について (2008)
  • Author(s): 齋藤剛, ら
  • Organizer: 第41回日本整形外科学会 骨軟部腫瘍学術集会
  • Place of Presentation: 浜松
• [Presentation] Desmoplastic small round cell tumorにおけるFGFR4シグナルの治療標的としての可能性 (2008)
  • Author(s): 齋藤剛, ら
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 乳房に発生した顆粒細胞腫の1例 (2008)
  • Author(s): 稲垣敦史、長尾俊孝、桑原淳、片桐仁子、武田由美子、室井昭、齋藤剛, ら
  • Organizer: 第47回日本臨床細胞学会秋期大会
  • Place of Presentation: 東京
• [Presentation] A演説「滑膜肉腫における上皮間葉および間葉上皮移行」-SYT-SSX融合遺伝子と細胞間接着蛋白の関与- (2008)
  • Author(s): 齋藤剛
  • Organizer: 第54回日本病理学会秋期特別総会
  • Place of Presentation: 松山
• [Remarks] ホームページ等
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/jintai_byori/index.html